Investigation of the anticancer activity of zinc(II) complex containing Schiff base on colorectal cancer cell

Yıl 2024, Cilt: 14 Sayı: 4, 1039 - 1048, 15.12.2024

Asuman Uçar , Sultan Çelik , Suray Pehlivanoğlu

https://doi.org/10.17714/gumusfenbil.1486484

Öz

Colorectal cancer is a common heterogeneous disease worldwide and is difficult to treat and ranks second in terms of cancer-related deaths. The majority of cases are at an advanced stage at the time of diagnosis and occurs resistance to the conventional chemotherapeutic agents. In this study, N,N'-bis[(2-hydroxy-1-naphthalenyl)methylene]-propane-1,3 diamine ligand (H2L) and its Zn(II) complex were synthesized as a potential agent, starting from 1,3-diaminopropane and 2-hydroxy-1-naphthaldehyde. The compounds were characterized by 1H-NMR, 13C-NMR, FT-IR, UV and their possible therapeutic effects on HT29 colorectal cancer cells were investigated. The effects of H2L and Zn(II) complex on HT29 cell viability were evaluated by MTT assay. IC50 values of H2L and Zn(II) complex on HT29 cells were calculated as 117.1 µg/ml and 17.03 µg/ml, respectively. Compared to its ligand, the Zn(II) complex significantly reduced viability and promoted apoptotic morphologies in the cells. According to the results of this study, Zn(II) complex may be a promising chemotherapeutic with therapeutic potential. More comprehensive studies are needed in the future to elucidate the mechanism of action of the Zn(II) complex on colorectal cancer cells.

Anahtar Kelimeler

Colorectal cancer, Schiff base, Cytotoxicity, Zn(II) complex

Schiff bazı içeren çinko(II) kompleksinin kolorektal kanser hücreleri üzerindeki antikanser aktivitesinin incelenmesi

Öz

Kolorektal kanser, Dünya’da sık görülen ve kansere bağlı ölümler açısından ikinci sırada yer alan, tedavisi zor heterojen bir hastalıktır. Hastaların çoğunluğu tanı anında ileri evrededir ve kullanılan kemoterapötik ajanlara direnç söz konudur. Bu çalışmada potansiyel bir ajan olarak, 1,3-diaminopropan ve 2-hidroksi-1-naftaldehitten yola çıkarak N,N'-bis[(2-hidroksi-1- naftalenil)metilen]-propan-1,3-diamin ligandı (H2L) ve onun Zn(II) kompleksi sentezlenmiştir. Bileşikler 1H-NMR, 13C-NMR, FT-IR, UV ile karakterize edilmiştir ve HT29 kolorektal kanser hücreleri üzerine olan olası terapötik etkileri araştırılmıştır. H2L ve Zn(II) kompleksinin HT29 hücre canlılığı üzerine etkileri MTT testi ile değerlendirilmiştir. H2L ve Zn(II) kompleksinin HT29 hücrelerinde IC50 değerleri sırasıyla 117.1 µg/ml ve 17.03 µg/ml olarak hesaplanmıştır. Zn(II) kompleksi ligandına kıyasla hücre canlılığını anlamlı derecede azaltmış ve hücresel apoptotik morfolojilerin oluşmasını tetiklemiştir. Çalışmanın sonuçlarına göre, Zn(II) kompleksi umut veren ve terapötik potansiyeli olan bir kemoterapötik olabileceğini destekler niteliktedir. Gelecekte Zn(II) kompleksinin kolorektal kanser hücreleri üzerindeki etki mekanizmasını aydınlatacak daha kapsamlı çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

Kolorektal kanser, Schiff bazı, Sitotoksisite, Zn(II) kompleksi

Teşekkür

A.K. Necmettin Erbakan Üniversitesi Eğitim Fakültesi, Kimya Eğitimi Bölümü Araştırma Laboratuvarında çalışma fırsatı sağladığı için, Prof. Dr. Emine Güler Akgemci’ye teşekkürler.

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