Comparing Autosomal SSR and PCR-RFLP Markers to Determine Phylogenetic Relationship Based on Genetic Distances in Livestock
Öz
Objective: Many molecular tools are available to analyse phylogenetic relationships in livestock. Nowadays, Simple Sequence Repeats and Single Nucleotide Polymorphisms are commonly used molecular techniques to determine phylogenetic relationships in livestock breeds or types. However, alternative molecular techniques may be preferred to conduct phylogenetic analysis in case of limiting conditions such as budget and time. In this context, in the present study, Simple Sequence Repeats and Polymerase Chain Reaction- Restriction Fragment Length Polymorphism techniques were compared to reveal phylogenetic relationship based on genetic distances.
Material and Methods: In this study, 11 different layer pure chicken lines represented by 30 individuals for each line were genotyped based on 11 Polymerase Chain Reaction- Restriction Fragment Length Polymorphism and 17 Simple Sequence Repeats loci to analyse phylogenetic relationship.
Results: Both techniques showed almost similar results in terms of Unweighted Pair Group Method with Arithmetic Mean dendrogram created based on genetic distances. White and brown chicken lines were separated by both Polymerase Chain Reaction- Restriction Fragment Length Polymorphism and Simple Sequence Repeats techniques in harmony with their genetic origins and breeding history.
Conclusion: It is suggested that Polymerase Chain Reaction- Restriction Fragment Length Polymorphism technique may be preferred to analyse phylogenetic relationship based on genetic distance, when the budget, time and laboratory infrastructure are limited.
Anahtar Kelimeler
Farm animals, UPGMA dendrogram, molecular markers
Destekleyen Kurum
The Scientific Research Projects Coordination Unit of Akdeniz University
Proje Numarası
2013.03.0121.001; FBA-2015-756 and FYL-2017-2912
Teşekkür
The data of the present study were obtained from different projects supported by the Scientific Research Projects Coordination Unit of Akdeniz University (Project Number: 2013.03.0121.001; FBA-2015-756 and FYL-2017-2912). The authors grateful to the Scientific Research Projects Coordination Unit of Akdeniz University for financial support and to Ankara Poultry Research Institute for blood samples.
Kaynakça
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