Kolon Kanseri Hücrelerinde (DLD-1) Pirolidin Türevi Bileşiğin Antiproliferatif Aktivitesi

Öz

Amaç: Anti-kanser ilaç araştırmaları, çeşitli kanser türlerinde kemoterapötik tedavilerde önemli bir rol oynamaktadır. Pirolidin türevi bileşiklerin birçok araştırmacı tarafından güçlü bir anti-kanser bileşiği olduğu bildirilmiştir. Antiproliferatif aktiviteye sahip yeni ilaç adayları olduğu düşünülen pirolidin türevi bileşiklerin DLD-1 ve CCD-18CO hücre hatları üzerindeki etkilerinin araştırılması amaçlanmıştır.

Gereç ve Yöntemler: Pirolidin türevli bileşiklerin antiproliferatif aktivitesi DLD-1 (insan kolon kanseri) ve CCD-18CO (normal kolon fibroblast) hücre hatlarında karşılaştırılarak farklı konsantrasyonlarda (25-100 uM) MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5 Diphenyltetrazolium bromide) ve RTCA (gerçek zamanlı hücre analizi) deneyleri ile 24 saat boyunca incelendi. MTT deneyinde veri setleri arasındaki farkların önemi, DLD-1 ve CCD-18CO hücre hatlarında SPSS 20.0 programıyla ANOVA ile istatistiksel olarak analiz edildi.

Bulgular: Pirolidin türevi bileşiklerin MTT yöntemi ile negatif kontrole göre DLD-1 kanser hücrelerinin sayısını azalttığı ve RTCA test sonuçlarına göre ise DLD-1 hücresinin baskılandığı belirlendi. Bu nedenle, bileşiklerin hücre proliferasyonunu inhibe ettiği ve antiproliferatif aktiviteye sahip olduğu gösterilmiştir.

Sonuç: Pirolidin türevi bileşiklerin DLD-1 kanser hücrelerinde antiproliferatif aktivite çalışmaları için ilk adım olabileceği ve gelecekteki çalışmalara rehberlik edeceği düşünülmektedir.

Anahtar Kelimeler

• Antiproliferatif Aktivite
• Kolon Kanseri
• MTT
• Pirolidin
• RTCA

Antiproliferative Activity of Pyrrolidine Derivatives compound in Colon Cancer Cells

Öz

Objective: Anti-cancer drug research plays an important role for chemotherapeutic treatments in various types of cancer. Pyrolidine derived compounds have been reported by many researchers to be a potent anti-cancer compound. It is aimed to investigate the effects of pyrolidine-derived compounds that are thought to be new drug candidates with antiproliferative activity on DLD-1 and CCD-18CO cell lines.

Material and Methods: The antiproliferative activity of the pyrrolidine-derived compound was determined for 24 hours at different concentrations (25-100 µM) on DLD-1 (human colon cancer) and CCD-18CO (normal colon fibroblast) cell lines by comparing MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5 Diphenyltetrazolium bromide) and RTCA (real-time cell analysis) assays. The significance of the differences between data sets in the MTT assay was analyzed statistically by ANOVA with SPSS 20.0 program for DLD-1 and CCD-18CO cell lines.

Results: It has been determined that pyrrolidine-derived compounds reduce the number of DLD-1 cancer cells according to negative control with the MTT method and suppress the DLD-1 cell according to the RTCA assay results. Thus, the compounds have been shown to inhibit cell proliferation and have antiproliferative activity.

Conclusion: Pyrrolidine-derived compounds will be the first step for antiproliferative activity studies in DLD-1 cancer cells and will guide the next studies.

Anahtar Kelimeler

• Antiproliferative Activity
• Colon cancer
• MTT
• Pyrrolidine
• RTCA

Kaynakça

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