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Yanık Sonrası Gelişen Bakteriyel Translokasyon Üzerine Amifostin’in (WR-2721) Etkisi

Yıl 2021, Cilt 3, Sayı 3, 71 - 75, 14.10.2021
https://doi.org/10.52827/hititmedj.967106

Öz

Amaç: Bağırsak epitel bariyerinin bozulmasının yanık yaralanmasını takiben meydana geldiği gösterilmiştir. Bu süreç, patojenlerin bağırsak lümeninden sistemik dolaşıma ve uzak organlara yer değiştirmesine yol açarak sepsis riskini artırır. Bu çalışmanın amacı, sıçan yanığı yaralanma modelinde amifostin'in (WR-2721) bakteriyel translokasyon üzerindeki etkisini incelemektir.

Gereç ve Yöntemler: Toplam 27 erkek Wistar albino sıçanı dokuzlu üç gruba ayrıldı. Grup I bir kontrol grubuydu. Grup II ve grup III, toplam vücut yüzey alanının %30'u üzerinde üçüncü derece yanıklara maruz bırakıldı ve grup III'e intraperitoneal olarak 200 ml/kg amifostin uygulandı, ardından üçüncü derece yanıklardan sonra 10 ml/kg/gün idame dozu uygulandı. 48 saat sonra karaciğer, dalak, mezenterik lenf düğümleri ve çekumdan doku ve kan örnekleri alındı ve kültür ekimi yapıldı.

Bulgular: Kan kültürleri tüm gruplarda negatifti. Kontrol grubunda kolonizasyon sadece çekumda görülürken, grup II ve III'te karaciğer, dalak, mezenterik lenf nodları ve çekumda kolonizasyon tespit edildi. Bakteriyel kolonizasyon en sık çekum ve mezenterik lenf düğümlerinde bulunurken, grup II ve III arasında çekum (P = 0.298) ve mezenterik lenf düğümlerinde (P = 0.418) bakteri sayıları önemli ölçüde farklılık göstermedi.

Sonuç: Amifostin tek başına yanık yaralanmaları ile ilişkili bakteriyel translokasyonu kontrol etmede etkili değildir. Bakteriyel translokasyonu etkileyen bir dizi faktör olduğu için bu sonuçlar dikkatle yorumlanmalıdır.

Kaynakça

  • 1. Church D, Elsayed S, Reid O, Winston B, Lindsay R. Burn wound infections. Clin Microbiol Rev. 2006;19:403-434.
  • 2. Stoecklein VM, Osuka A, Lederer JA. Trauma equals danger--damage control by the immune system. J Leukoc Biol. 2012;92:539-551.
  • 3. Shankar R, Melstrom KA, Jr., Gamelli RL. Inflammation and sepsis: past, present, and the future. J Burn Care Res. 2007;28:566-571.
  • 4. Magnotti LJ, Deitch EA. Burns, bacterial translocation, gut barrier function, and failure. J Burn Care Rehabil. 2005;26:383-391.
  • 5. MacFie J, O'Boyle C, Mitchell CJ, Buckley PM, Johnstone D, Sudworth P. Gut origin of sepsis: a prospective study investigating associations between bacterial translocation, gastric microflora, and septic morbidity. Gut. 1999;45:223-228.
  • 6. Choudhry MA, Rana SN, Kavanaugh MJ, Kovacs EJ, Gamelli RL, Sayeed MM. Impaired intestinal immunity and barrier function: a cause for enhanced bacterial translocation in alcohol intoxication and burn injury. Alcohol. 2004;33:199-208.
  • 7. Nicolatou-Galitis O, Sarri T, Bowen J, et al. Systematic review of amifostine for the management of oral mucositis in cancer patients. Support Care Cancer. 2013;21:357-364.
  • 8. Grdina DJ, Kataoka Y, Murley JS. Amifostine: mechanisms of action underlying cytoprotection and chemoprevention. Drug Metabol Drug Interact. 2000;16:237-279.
  • 9. Tas S, Ozkul F, Arik MK, Kiraz A, Vural A. The effect of amifostine on bacterial translocation after radiation induced acute enteritis. Acta Cir Bras. 2016;31:156-160.
  • 10. Gilpin DA. Calculation of a new Meeh constant and experimental determination of burn size. Burns. 1996;22:607-611.
  • 11. Earley ZM, Akhtar S, Green SJ, et al. Burn Injury Alters the Intestinal Microbiome and Increases Gut Permeability and Bacterial Translocation. PLoS One. 2015;10:e0129996.
  • 12. Kompan L, Kremzar B, Gadzijev E, Prosek M. Effects of early enteral nutrition on intestinal permeability and the development of multiple organ failure after multiple injury. Intensive Care Med. 1999;25:157-161.
  • 13. Wilmore DW, Smith RJ, O'Dwyer ST, Jacobs DO, Ziegler TR, Wang XD. The gut: a central organ after surgical stress. Surgery. 1988;104:917-923.
  • 14. Carrico CJ, Meakins JL, Marshall JC, Fry D, Maier RV. Multiple-organ-failure syndrome. Arch Surg. 1986;121:196-208.
  • 15. Vaishnavi C. Translocation of gut flora and its role in sepsis. Indian J Med Microbiol. 2013;31:334-342.
  • 16. Santini V. Amifostine: chemotherapeutic and radiotherapeutic protective effects. Expert Opin Pharmacother. 2001;2:479-489.

Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study

Yıl 2021, Cilt 3, Sayı 3, 71 - 75, 14.10.2021
https://doi.org/10.52827/hititmedj.967106

Öz

Objective: Disruption of the intestinal epithelial barrier has been shown to occur following burn injury. This process can lead to translocation of pathogens from the gut lumen to the systemic circulation and distant organs thereby increasing the risk for sepsis. The aim of this study was to examine the effect of amifostine (WR-2721) on bacterial translocation in a rat burn injury model.

Material and Method: A total of 27 male Wistar albino rats were divided into three groups of nine. Group I was a control group. Group II and Group III was subjected to third-degree burns over 30% of the total body surface area, and group III was administered amifostine 200 ml/kg intraperitoneally, followed by a 10 ml/kg/day maintenance dose after undergoing third-degree burns. After 48 hours, tissue and blood samples were obtained and cultured from the liver, spleen, mesenteric lymph nodes, and cecum.

Results: Blood cultures were negative in all groups. In the control group, colonization appeared only in the cecum, but in groups II and III, colonization was found in the liver, spleen, mesenteric lymph nodes, and cecum. While bacterial colonization was most frequently found in the cecum and mesenteric lymph nodes, bacterial counts did not significantly differ in the cecum (P = 0.298) and mesenteric lymph nodes (P = 0.418) between groups II and III.

Conclusion: Amifostine alone is not effective in controlling bacterial translocation associated with burn injuries. These results should be interpreted with caution as there are a number of factors that affect bacterial translocation.

Kaynakça

  • 1. Church D, Elsayed S, Reid O, Winston B, Lindsay R. Burn wound infections. Clin Microbiol Rev. 2006;19:403-434.
  • 2. Stoecklein VM, Osuka A, Lederer JA. Trauma equals danger--damage control by the immune system. J Leukoc Biol. 2012;92:539-551.
  • 3. Shankar R, Melstrom KA, Jr., Gamelli RL. Inflammation and sepsis: past, present, and the future. J Burn Care Res. 2007;28:566-571.
  • 4. Magnotti LJ, Deitch EA. Burns, bacterial translocation, gut barrier function, and failure. J Burn Care Rehabil. 2005;26:383-391.
  • 5. MacFie J, O'Boyle C, Mitchell CJ, Buckley PM, Johnstone D, Sudworth P. Gut origin of sepsis: a prospective study investigating associations between bacterial translocation, gastric microflora, and septic morbidity. Gut. 1999;45:223-228.
  • 6. Choudhry MA, Rana SN, Kavanaugh MJ, Kovacs EJ, Gamelli RL, Sayeed MM. Impaired intestinal immunity and barrier function: a cause for enhanced bacterial translocation in alcohol intoxication and burn injury. Alcohol. 2004;33:199-208.
  • 7. Nicolatou-Galitis O, Sarri T, Bowen J, et al. Systematic review of amifostine for the management of oral mucositis in cancer patients. Support Care Cancer. 2013;21:357-364.
  • 8. Grdina DJ, Kataoka Y, Murley JS. Amifostine: mechanisms of action underlying cytoprotection and chemoprevention. Drug Metabol Drug Interact. 2000;16:237-279.
  • 9. Tas S, Ozkul F, Arik MK, Kiraz A, Vural A. The effect of amifostine on bacterial translocation after radiation induced acute enteritis. Acta Cir Bras. 2016;31:156-160.
  • 10. Gilpin DA. Calculation of a new Meeh constant and experimental determination of burn size. Burns. 1996;22:607-611.
  • 11. Earley ZM, Akhtar S, Green SJ, et al. Burn Injury Alters the Intestinal Microbiome and Increases Gut Permeability and Bacterial Translocation. PLoS One. 2015;10:e0129996.
  • 12. Kompan L, Kremzar B, Gadzijev E, Prosek M. Effects of early enteral nutrition on intestinal permeability and the development of multiple organ failure after multiple injury. Intensive Care Med. 1999;25:157-161.
  • 13. Wilmore DW, Smith RJ, O'Dwyer ST, Jacobs DO, Ziegler TR, Wang XD. The gut: a central organ after surgical stress. Surgery. 1988;104:917-923.
  • 14. Carrico CJ, Meakins JL, Marshall JC, Fry D, Maier RV. Multiple-organ-failure syndrome. Arch Surg. 1986;121:196-208.
  • 15. Vaishnavi C. Translocation of gut flora and its role in sepsis. Indian J Med Microbiol. 2013;31:334-342.
  • 16. Santini V. Amifostine: chemotherapeutic and radiotherapeutic protective effects. Expert Opin Pharmacother. 2001;2:479-489.

Ayrıntılar

Birincil Dil İngilizce
Konular Tıp
Bölüm Araştırma Makaleleri
Yazarlar

Necip ALTUNDAŞ Bu kişi benim
ATATÜRK ÜNİVERSİTESİ
0000-0002-5165-638X
Türkiye


Erdal KARAGÜLLE
BAŞKENT ÜNİVERSİTESİ
0000-0002-8522-4956
Türkiye


Emre KARAKAYA (Sorumlu Yazar)
BAŞKENT ÜNİVERSİTESİ
0000-0002-4879-7974
Türkiye

Yayımlanma Tarihi 14 Ekim 2021
Başvuru Tarihi 8 Temmuz 2021
Kabul Tarihi 9 Eylül 2021
Yayınlandığı Sayı Yıl 2021, Cilt 3, Sayı 3

Kaynak Göster

Bibtex @araştırma makalesi { hititmedj967106, journal = {Hitit Medical Journal}, issn = {}, eissn = {2687-4717}, address = {İkbalkent Kampüsü Ulukavak Mah. Çiftlik Çayırı Cad. No: 45 19040 Çorum}, publisher = {Hitit Üniversitesi}, year = {2021}, volume = {3}, pages = {71 - 75}, doi = {10.52827/hititmedj.967106}, title = {Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study}, key = {cite}, author = {Altundaş, Necip and Karagülle, Erdal and Karakaya, Emre} }
APA Altundaş, N. , Karagülle, E. & Karakaya, E. (2021). Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study . Hitit Medical Journal , 3 (3) , 71-75 . DOI: 10.52827/hititmedj.967106
MLA Altundaş, N. , Karagülle, E. , Karakaya, E. "Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study" . Hitit Medical Journal 3 (2021 ): 71-75 <https://dergipark.org.tr/tr/pub/hititmedj/issue/65429/967106>
Chicago Altundaş, N. , Karagülle, E. , Karakaya, E. "Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study". Hitit Medical Journal 3 (2021 ): 71-75
RIS TY - JOUR T1 - Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study AU - Necip Altundaş , Erdal Karagülle , Emre Karakaya Y1 - 2021 PY - 2021 N1 - doi: 10.52827/hititmedj.967106 DO - 10.52827/hititmedj.967106 T2 - Hitit Medical Journal JF - Journal JO - JOR SP - 71 EP - 75 VL - 3 IS - 3 SN - -2687-4717 M3 - doi: 10.52827/hititmedj.967106 UR - https://doi.org/10.52827/hititmedj.967106 Y2 - 2021 ER -
EndNote %0 Hitit Medical Journal Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study %A Necip Altundaş , Erdal Karagülle , Emre Karakaya %T Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study %D 2021 %J Hitit Medical Journal %P -2687-4717 %V 3 %N 3 %R doi: 10.52827/hititmedj.967106 %U 10.52827/hititmedj.967106
ISNAD Altundaş, Necip , Karagülle, Erdal , Karakaya, Emre . "Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study". Hitit Medical Journal 3 / 3 (Ekim 2021): 71-75 . https://doi.org/10.52827/hititmedj.967106
AMA Altundaş N. , Karagülle E. , Karakaya E. Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study. Hitit Med J. 2021; 3(3): 71-75.
Vancouver Altundaş N. , Karagülle E. , Karakaya E. Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study. Hitit Medical Journal. 2021; 3(3): 71-75.
IEEE N. Altundaş , E. Karagülle ve E. Karakaya , "Effect of Amifostine (WR-2721) on Bacterıal Translocation After Burn Injury: An Experımental Study", Hitit Medical Journal, c. 3, sayı. 3, ss. 71-75, Eki. 2021, doi:10.52827/hititmedj.967106
hmj@hitit.edu.tr