Optimization the Ultrasonic Assisted Extraction of Chlorogenic Acid from the Artichoke (Cynara Scolymus) Leaf with Response Surface Methodology and Total Phenolic Contents

Yıl 2020, Cilt: 48 Sayı: 2, 147 - 157, 19.04.2020

Yakup Atıcı , İbrahim Bulduk

https://doi.org/10.15671/hjbc.511586

Cited By: 1

Öz

Artichoke leaf
(Cynara Scolymus) extracts widely
used in medicine. Chlorogenic Acids have antioxidant, antibacterial and
antiviral properties is an compound.

In this study,
after the artichoke (Cynara scolymus)
leaves had  dried, powdered portions were
extracted to with water. The results had expressed as ppm after  extracts  were analyzed by HPLC. It were determined that
optimum conditions of extracting chlorogenic acid from Cynara Scolymus leaves with the ultrasonic assisted extraction with
Response Surface Method (RSM) based on Box-Behnken design. The experimental
conditions for this extraction efficiency were determined as extraction time
(15-45 minutes), temperature (30-70⁰C) and ultrasound power
(25-75%).  The optimum conditions were
determined as 47.65% ultrasound power, 35.18 minutes, 53.40⁰ C temperature.
The maximum yield of chlorogenic acid was determined to 10.05 ppm.

Also the total
phenolic content was measured by UV -Vis spectrometry in order to see antioxidant
activity.

Absorbance values
of samples which are took from each sample  were measured at 765 nm in UV spectrometer. Optimum
conditions were determined as 47.29% ultrasound power, 29.67 minutes, 55.33% ethanol
concentration. Total phenolic content were determined as 70.24 ppm. According
the to results ultrasonic-assisted extraction (UAE) showed can be an effective
method for the extraction of chlorogenic acid active ingredient.

Anahtar Kelimeler

Chlorogenic Acid, Artichoke leaf (Cynara scolymus), Extraction, Optimization, RSM

Kaynakça

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