Investigation of the Phytochemical Properties and In Vivo Diuretic Activity of Paliurus spina-christi Plant Seeds

Öz

Background: This study aimed to investigate the diuretic effect (DE) of the extract obtained from the Paliurus spina-christi (PSC) seeds in Wistar Albino rats. Materials and Methods: The study utilised 20 adult male Wistar Albino rats and employed P. spina-christi seeds as the plant material. The antioxidant potential of the extract was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, Ferric Reducing Antioxidant Power (FRAP), and Copper (II) Reduction Antioxidant Capacity (CUPRAC) methods. To determine the diuretic effect, five groups consisting of four rats each were established. The rats received 0.9% NaCl (C)(2.5 mL/100 g) as the negative control and furosemide (10mg/kg) as the positive control (F) orally. The treatment groupswere administered the plant extract orally at doses of 20 mg/kg(PSC-20), 40 mg/kg (PSC-40), and 60 mg/kg (PSC-60). The DEwas assessed by measuring the volume, pH, and specific gravity ofurine samples collected after short (6 hours) and long (24 hours)periods, as well as various clinical parameters. Results: The findings of the study indicated that the use ofP.spina-christi seed extract at doses of 20, 40, and 60 mg/kgsignificantly increased urine output, diuretic effect, and diureticactivity after both 6 and 24 hours. Conclusions: The P. spina-christi seed extract, rich inphenolic and flavonoid components with antioxidant properties,demonstrates a dose-dependent DE on renal functions,establishing a linear relationship between dosage and diureticresponse. Further studies are required to explore the metabolic,hormonal, and toxic effects of the plant seeds.

Anahtar Kelimeler

• Paliurus spina-christi
• Diuretic effect
• Flavonoid compounds
• Phenolic compounds

Paliurus Spina-Christi Bitki Tohumlarının Fitokimyasal Özelliklerinin ve In Vivo Diüretik Aktivitesinin Araştırılması

Öz

Amaç: Bu çalışmada Paliurus spina-christi (PSC) tohumlarından elde edilen ekstraktın diüretik etkisi Wistar Albino sıçanları kullanılarak araştırılması amaçlandı. Materyal ve metod: Çalışmada 20 adet yetişkin erkek Wistar Albino sıçan ve bitki materyali olarak P. spina-christi tohumLarı kullanıldı. Ekstraktın antioksidan potansiyeli, 1,1-difenil-2-pikrilhidrazil (DPPH) radikali giderme aktivitesi, Fe3+ indirgeme gücü (FRAP) ve Cu2+ indirgeme antioksidan kapasitesi (CUPRAC) yöntemLeri kullanılarak değerlendirildi. Diüretik etkinin belirlenmesinde her biri 4 sıçandan oluşan 5 grup oluşturuldu. Sıçanlara negatif kontrol olarak %0,9 NaCl 2,5 mL/100 g dozda (C) ve pozitif kontrol olarak Furosemid 10 mg/kg dozda (F) oral olarak verildi. Uygulama gruplarına ise 20 mg/kg (PSC-20), 40 mg/kg (PSC-40) ve 60 mg/kg (PSC-60) dozlarında bitki ekstraktı oral yoldan uygulandı. Diüretik etki, kısa (6 saat) ve uzun (24 saat) periyodlar sonrasında toplanan idrar örneklerinin hacim, pH ve özgül ağırlık değerlerinin yanı sıra çeşitli klinik parametrelerle değerlendirildi. Bulgular: Çalışma bulguları, P. spina-christi tohum ekstraktının 20, 40 ve 60 mg/kg dozlarında kullanımı sonrasında 6 ve 24 saatlik sürelerde idrar çıkışını, diüretik etkiyi ve diüretik aktiviteyi anlamLı bir şekilde artırdığı görüldü. Sonuç: Fenolik ve flavonoid bileşenler bakımından zengin antioksidan özelliklere sahip P. spina-christi tohum ekstraktının böbrek fonksiyonları üzerinde doza bağlı bir diüretik etki sağladığı ve doz ile diüretik yanıt arasında doğrusal bir ilişki olduğu belirlendi. Bitki tohumlarının metabolik, hormonal ve toksik etkilerinin araştırılması için ek çalışmalara ihtiyaç vardır.

Anahtar Kelimeler

• Paliurus spina-christi
• Diüretik etki
• Flavonoid madde
• Fenolik madde

Kaynakça

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