Urfa fıstığından (Pistacia vera L.) elde edilen çeşitli ekstraktların antikanser özelliklerinin incelenmesi

Öz

Amaç: Urfa Fıstığı (Pistacia vera L.) son yıllarda yapılan çalışmalarda; zengin fenolik bileşikler kaynağı olup, antioksidan potansiyeli en yüksek gıda ürünleri arasında yer almaktadır. Ayrıca, antosiyanin, isoflavonlar, gallik asit, kateşin ve epikateşin gibi antikanser potansiyeline sahip yüksek antioksidan etki gösteren bileşikler içerdiği tespit edilmiştir. Bu çalışmanın amacı, çeşitli bitki kısımları kullanılarak ekstrakte edilen Pistacia vera L‘nın antikanser potansiyelinin incelenmesidir.

Materyal ve Metod: P.vera L’nın meyve sapı, kırmızı dış kabuk örtüsü ve reçinesinden elde edilen metanol ekstrelerinin insan prostat (PC3, DU-145), meme (MDA-MB-231, MCF-7) kanser hücreleri ve normal hücreler (PNT1-A ve CRL-4010) üzerinde sitotoksik etkisi MTT metodu ile incelendi.

Bulgular: Çalışma sonucunda tüm bitki ekstrelerinin düşük dozlarda kanser hücreleri üzerinde sitotoksik etki gösterirken normal hücreler üzerinde ise yüksek dozlarda etkili olduğu tespit edildi. En güçlü sitotoksik etkiyi ise reçine kısmının prostat kanseri (PC3) üzerinde gösterdiği (IC50: 6,092 µg/ml) belirlenmiştir.

Sonuç: Bu çalışma kanser tedavisinde kullanılabilecek aktif bileşiklerin tespiti için yeni bir kaynak oluşturup, sonraki yapılacak çalışmalar için teşvik edici olacaktır.

Anahtar Kelimeler

• Urfa Fıstığı,Pistacia vera,Antikanser

Examination of anticancer properties of the various extracts of obtained from Urfa pistachio (Pistacia Vera L.)

Öz

Background: Studying for years, Urfa pistachio (Pistacia vera L.) has rich phenolic compounds and is classified among the highest antioxidant food products. Anthocyanins in its structure contain highly antioxidant compounds such as isoflavones, gallic acid, catechin and epicatechin, with anticancer potency. The purpose of this study is to examine the anticancer potential of the extracts of the various parts of Pistacia vera L.

Material and Methods: The cytotoxic effects of methanol extracts obtaining from fruit stem, red outer shell cover and resin of P.vera  on the  human prostate (PC3, DU-145), breast cancer (MDA-MB-231, MCF-7)  and  normal cells (PNT1-A and CRL-4010) were examined by MTT method.

Results: All plant extracts showed cytotoxic effects on cancer cells at allow dose; on the other hand, hardly have indicated the high dose of Pistacia vera L. a cytotoxic effect on the normal cells. The resin fraction was the part that was recorded as showing the strongest cytotoxicity on prostate cancer (PC3).

Conclusion: This work will create a new resource for the detection of active compounds that can be used in cancer treatment and will be an incentive for future work.

Anahtar Kelimeler

• Urfa pistachio,Pistacia vera,Anticancer

Kaynakça

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