The Effects of Coenzyme Q10 on Bisphenol A-Induced Pancreatic Changes in RatsPP

Öz

Background: In the study, it was aimed to determine the changes in the pancreas of rats exposed to bisphenol A (BPA) and to reveal the effects of coenzyme Q10 (CoQ10) on these changes.
Materials and Methods: The rats were randomly divided into five groups as follows: control, sham, CoQ10, BPA and CoQ10+BPA. The applications of CoQ10 and BPA continued for fourteen days. Crossmon’s triple staining method was applied to the pancreas sections for histopathological and histomorphological examinations. Also, estrogen receptor α (ERα) immunoreactivity on the pancreas tissue was examined immunohistochemically.
Results: It was determined that histopathological changes in the endocrine part of the pancreas and ERα immunoreactivity on the pancreatic beta cells significantly increased in BPA group compared to control group. But a significant difference wasn't found between the groups in terms of histomorphological findings. On the other hand, a significant effect of CoQ10 wasn’t observed on the parameters analyzed in the study.
Conclusion: BPA might cause structural and functional disorders in the pancreas. Also, it might change ERα immunoreactivity, too. However, further studies are required to reveal the possible protective effects of CoQ10 in the pancreas of rats exposed to BPA.

Sıçanlarda Bisfenol A’nın Neden Olduğu Pankreatik Değişimler Üzerine Koenzim Q10’un Etkileri

Öz

Amaç: Çalışmada bisfenol A (BPA)'ya maruz bırakılan sıçanların pankreasındaki değişikliklerin belirlenmesi ve bu değişiklikler üzerine koenzim Q10 (KoQ10)'un etkilerinin ortaya çıkarılması amaçlandı.
Materyal ve metod: Sıçanlar kontrol, sham, KoQ10, BPA ve KoQ10+BPA olmak üzere rastgele beş gruba ayrıldı. KoQ10 ve BPA uygulamaları on dört gün boyunca devam etti. Histopatolojik ve histomorfolojik incelemeler için pankreas kesitlerine Crossmon’un üçlü boyama yöntemi uygulandı. Ayrıca pankreas dokusunda immünohistokimyasal olarak östrojen reseptör α (ERα) immünoreaktivitesi incelendi.
Bulgular: Pankreasın endokrin bölümündeki histopatolojik değişiklikler ile pankreatik beta hücrelerindeki ERα immünoreaktivitesinin kontrol grubuna göre BPA grubunda anlamlı bir şekilde arttığı tespit edildi. Ancak histomorfolojik bulgular açısından gruplar arasında anlamlı bir fark bulunmadı. Diğer taraftan, çalışmada incelenen parametreler üzerinde KoQ10'un anlamlı bir etkisi gözlenmedi.
Sonuç: BPA, pankreasta yapısal ve fonksiyonel bozukluklara neden olabilir. Ayrıca ERα immünoreaktivitesini de değiştirebilir. Bununla birlikte, KoQ10'un BPA'ya maruz kalan sıçanların pankreasındaki muhtemel koruyucu etkilerini ortaya çıkarmak için daha ileri çalışmalar gereklidir.

Anahtar Kelimeler

• Beta hücresi
• Bisfenol A
• Koenzim Q10
• Östrojen reseptör α
• Pankreas

Kaynakça

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