Migren Hastalığının Patogenezinde Oksidatif Stres, Damar Fizyopatolojisini Etkileyen Faktörler ve Enflamasyonun Rolü

Yıl 2021, , 75 - 81, 28.04.2021

Eray Metin Güler , Ülker Anadol Hayriye Polat Ahmet Kılınç Destina Yalçın , A. Suha Yalçın

https://doi.org/10.35440/hutfd.883824

Öz

Amaç: Çalışmamızda migren tanısı konmuş hastalar ile sağlıklı kontrol grubu arasında oksidatif stres, damar fizyopatolojisi ve enflamasyon biyobelirteçleri açısından bir fark olup olmadığının araştırılması amaçlandı.
Materyal ve Metod: SBÜ Ümraniye Eğitim Araştırma Hastanesi Nöroloji Polikliniğine başvuran, 18 - 49 yaş arasında olup migren tanı kriterlerine uyan ve sistemik herhangi bir hastalığı olmayan 27 hasta ile 27 sağlıklı kişiden kan ve idrar örnekleri alındı. İdrarda malondialdehit, eritrositlerde glutatyon, glutatyonla ilgili enzimler, süperoksit dismutaz, katalaz, malondialdehit ve protein karbonilleri, plazmada malondialdehit, bilirubin, ürik asit ve albümin gibi oksidatif stres biyobelirteçlerine, damar fizyopatolojisi biyobelirteçlerinden trombosit ve fibrinojene, enflamasyon biyobelirteçlerinden ise interkökin (IL) 1β, IL6, IL10, tümör nekrozis faktör (TNF) α, c reaktif protein (CRP) ve ferritin düzeyleri ölçüldü.
Bulgular: Hasta grubunda glutatyon ve glutatyonla ilgili enzimlerin yanında süperoksit dismutaz ve katalaz değerleri kontrol grubuna kıyasla istatistiksel olarak anlamlı düşük (p<0,001) bulundu. Plazma albümin düzeylerinde gruplar arasında istatistiksel fark görülmedi. Ürik asit ve total bilirubin düzeylerinde ise hasta grubundaki düzeyler istatistiksel olarak anlamlı yüksek (p<0,001) bulundu. Benzer şekilde oksidatif hasar belirteçleri olan protein karbonilleri ile plazma, eritrosit ve idrar malondialdehit düzeyleri hasta grubunda istatistiksel olarak anlamlı yüksek (p<0,001) bulundu. Damar fizyopatolojisi belirteçlerinden trombosit sayısı ve fibrinojen düzeylerinin hasta grubunda anlamlı olarak arttığı (p<0,001) gözlendi. Enflamasyon belirteçlerinden IL1β, IL6, IL10 ve TNFα düzeyleri hasta grubunda istatistiksel olarak anlamlı yüksek (p<0,001) bulunurken, CRP ve ferritin düzeyleri düşüktü.
Sonuç: Migren hastalarında oksidatif stres, damar fizyopatolojisi ve enflamasyon belirteçleri birlikte değerlendirildiğinde, hastalardaki baskılanan ve azalan antioksidan düzeylerinin oksidatif stresi arttırdığı dolayısıyla enflamasyon ve damar fizyopatolojisi değişikliklerine neden olduğu sonucuna varıldı.

Anahtar Kelimeler

damar fizyopatolojisi, enflamasyon, migren, oksidatif stres

Destekleyen Kurum

Marmara Üniversitesi Bilimsel Araştırma Projeleri Birimi

Proje Numarası

SAG-C-YLP-110412-0070

Teşekkür

Makalemiz Eray Metin Güler’in yüksek lisans tezinden üretilmiştir. Ayrıca, 6-11 Temmuz 2013 tarihinde gerçekleşen Federation of European Biochemical Societies Congress toplantısında “The Role of Oxidative / Antioxidative Balance, Vascular Pathophysiology and Inflammation in Migraine” başlıklı poster olarak sunulmuştur.

Role of oxidative stress, factors affecting vascular physiopathology and inflammation in the pathogenesis of migraine disease

Öz

Background: We compared oxidative stress, vascular pathophysiology, and inflammation markers of migraine patients with healthy volunteers.
Material and Method: Blood and urine samples were obtained from 27 healthy individuals and 27 patients with a diagnosis of migraine who applied to Neurology Outpatient Clinics of Umraniye Research and Training Hospital. Participants were aged between 18 – 49 years. Patients had their diagnosis established prior to the study, and the volunteers in the control group had no systemic disease or relevant disorders. Urine samples were tested for malondialdehyde while erythrocytes were investigated for glutathione, glutathione related enzymes, superoxide dismutase, catalase, malondialdehyde, and protein carbonyls. Plasma samples were analyzed for malondialdehyde, bilirubin, uric acid, and albumin as oxidative stress parameters. Thrombocyte count and fibrinogen levels were measured for vascular physiopathology, and IL1β, IL6, IL10, TNFα, CRP, and ferritin were used as inflammation markers.
Results: In addition to glutathione and glutathione-related enzymes, superoxide dismutase and catalase values were found to be statistically significantly lower (p<0.001) in the patient group. Albumin levels were similar in both groups, whereas uric acid and bilirubin levels were significantly higher (p<0.001) in the patient group. Similarly, protein carbonyls, which are oxidative damage markers, as well as urine, plasma, and erythrocyte malondialdehyde levels were significantly higher (p<0.001) in the patient group. Thrombocyte count and fibrinogen levels, both of which are vascular physiopathology markers, were found to increase in the patient group (p<0.001). The participants in the patient group had significantly higher (p<0.001) levels of IL1β, IL6, IL10, and TNFα as inflammation markers, on the other hand, CRP and ferritin levels were lower.
Conclusion: Considering oxidative stress, vascular physiopathology, and inflammation markers as a whole, we suggest that patients with migraine had increased oxidative stress due to suppressed and decreased levels of antioxidants and consequently had inflammatory and vascular changes.

Anahtar Kelimeler

Vascular Physiopathology, Inflammation, Migraine, Oxidative stress

Proje Numarası

SAG-C-YLP-110412-0070

Kaynakça

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