Ratlarda Parasetamol ile Oluşturulan Hepatotoksisite Üzerine Taraxacum officinale Etanol Ekstraktının Etkisi

Abstract

Ratlarda parasetamol ile oluşturulan hepatotoksisite üzerine Taraxacum officinale etanol ekstraktının kan ve karaciğer dokusundaki bazı biyokimyasal parametrelere ve karaciğer dokusunun histopatolojisi üzerine etkileri araştırıldı. Bu çalışmada, 5 aylık yaşta 36 adet Sprague Dawley rat kullanıldı. Sprague Dawley ratlar rastgele her grupta 6 rat olacak şekilde 6 gruba ayrıldı. Parasetamol (PARA) verilecek gruplardaki hayvanlar 24 saat aç bırakılıp ekstrakt verildikten 1 saat sonra 2 g/kg oral olarak (p.o.) PARA verildi. 1.Grup (Kontrol Grubu), %5’lik DMSO intraperitoneal (i.p.), 2.Grup (TOE1 Grubu), 200 mg/kg/gün/i.p. Taraxacum officinale ekstraktı (TOE) %5’lik DMSO’da çözülerek i.p, 3.Grup (TOE2 Grubu), 250 mg/kg/gün/i.p. Taraxacum officinale ekstraktı, 4.Grup (PARA Grubu), 2 g/kg/p.o. Parasetamol, 5.Grup (PTOE1 Grubu), Parasetamol 2 g/kg/gün/p.o. + TOE 200 mg/kg/gün/i.p, 6.Grup (PTOE2 Grubu), Parasetamol 2 g/kg/gün/p.o. + TOE 250 mg/kg/gün/i.p 8 gün boyunca uygulandı. Çalışma sonunda ratlardan alınan kan ve karaciğer dokusundan biyokimyasal ve histopatolojik analizler yapıldı. Parasetamol grubunda plazma AST, ALT, ALP, MDA, CAT ve nitrit düzeyleri; karaciğer MDA, nitrit ve nitrat düzeyleri kontrol grubuna göre artarken (P<0.001); plazma GSH (P<0.001), SOD (P<0.001) ve GPx (P<0.05) düzeyleri; karaciğer GSH, CAT ve SOD (P<0.001) düzeyleri anlamlı olarak azaldı. Parasetamolün ratlarda oluşturduğu hepatotoksisiteyi Taraxacum officinale bitkisinin toprak üstü aksamlarından yapılan etanol ekstraktının 200 mg/kg uygulanan miktarının hepatotoksisiteyi anlamlı olarak azalttığı ve lipid peroksidasyonu önlediği, TOE 250 mg/kg uygulanan hayvanlarda toksik etki yaptığı tespit edildi. Elde edilen ekstraktın hepatotoksisite üzerine ve plazma ve karaciğer biyokimyasal parametreleri üzerine olumlu etkilerinin olduğu saptansa bile karaciğerde oluşan hasarı düzeltmek için zamana gereksinim duyulduğu kanaatine varıldı.

Keywords

• Antioksidan,Ekstrakt,Lipid peroksidasyonu,Parasetamol,Taraxacum officinale

The Effect of Taraxacum officinale Ethanol Extract on Paracetamol Induced Hepatotoxicity in Rats

Abstract

In this study was investigated on the effects on the some biochemical and histopathological parameters in plasma and liver tissue of Taraxacum officinale ethanol extract of paracetamol induced hepatotoxicity in rats. In this study was utilized 36 Sprague Dawley male rats, aged 5 months. Rats were divided 6 groups of 6 rats each, randomly. Animals of paracetamol administered were fasted 24 hour and 2 g/kg peros (p.o) Paracetamol was given after the 1 hour extract was given. 1.Group Control  5% DMSO intraperitoneal (i.p), 2. Group TOE1 200 mg/kg/day/i.p. Taraxacum officinale extract (TOE) was dissolved in  5% DMSO in distilled water, i.p., 3. Group TOE2 250 mg/kg/day/i.p. Taraxacum officinale extract, 4. Group PARA 2 g/kg/p.o. Paracetamol, 5. Group PTOE1; Paracetamol 2 g/kg/day/p.o.+ TOE 200 mg/kg/day/i.p, 6. Group PTOE2 Paracetamol 2 g/kg/day/p.o.+ TOE 250 mg/kg/day/i.p were administered for 8 day. At the end of the study biochemical and histopatological analyses were made from sample of blood and liver tissue. Plasma AST, ALT, ALP, MDA, CAT and Nitrite levels; liver tissue Nitrite and Nitrate levels were increased (P<0.001); plasma GSH (P<0.001), SOD (P<0.001) and GPx levels (P<0.05); liver tissue GSH, CAT, and SOD levels (P<0.001) were decreased significantly in paracetamol group compared with control group. Paracetamol in rats formed hepatotoxicity Taraxacum officinale Wig. aerial part made ​​from ethanol as extract is used TOE 100 mg/kg of the amount of hepatotoxicity significantly decreased and prevented lipid peroxidation. Toxic effect was determined in animals administered TOE 250 mg/kg. Even if the obtained extract had positive effects on hepatotoxicity and biochemical parameters in liver and plasma, it was concluded that it was needed to for repair the damage of the liver.

Keywords

• Antioxidant,Extract,Lipid peroxidation,Paracetamol,Taraxacum officinale

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