Şanlıurfa’da Tüketime Sunulan Koyun Peynirlerinde İnek Sütü Varlığının Belirlenmesi
Yıl 2017,
Cilt: 6 Sayı: 2, 163 - 166, 27.12.2017
Serap Kılıç Altun
,
Hisamettin Durmaz
Öz
Koyun
peyniri ülkemizde tercih edilen peynir türlerindendir. Koyun sütünün gerek
pahalı gerekse temin edilmesinin daha zor olması nedeniyle koyun peyniri
üretiminde inek sütü ilavesi yapılmaktadır. Bu çalışmada, Şanlıurfa ilinde
perakende satışa sunulan 45 adet ambalajlı, 45 adet ambalajsız olmak üzere toplam
90 adet koyun peynir örneğinde ELISA metodu ile inek sütü varlığının tespiti
amaçlandı. ELISA metoduna göre koyun peynir örneklerinin
içerdiği inek sütü oranları %0.1’in altında, % 1’in altında ve %1’in üzerinde
olmak üzere sınıflandırıldı. İncelenen toplam 90 adet koyun
peyniri örneğinin 7’sinde (% 7.7) % 0.1’in altında, 53’ünde (% 58.8) % 1’in
altında ve 30’unda (% 33.3) ise % 1’in üzerinde inek sütü bulunduğu tespit
edildi. Süt ve süt ürünlerinde hileyi önlemek için yanıltıcı
etiketlemeden koruyabilecek analitik prosedürlerin yaygınlaştırılması gerektiği
kanaatine varıldı.
Kaynakça
- Addeo F, Moio L, Chianese L, Nota G, 1989: Evaluation of bovine and water buffalo milk in mixtures of liquid milk and Mozzarella cheese by gel isoelectric focusing. Italian J Food Sci, 1, 71-80.
- Anonim, 2001: European Commission, 2001: EC 213/2001 Methods forthe analysis and quality evaluation of milk and milkproducts. Official Journal of the European Communities, 44, L37/1-L37/99.
- Borkova M, Snaselova J, 2005: Possibilities of different animal milk detection in milk and dairy products–a review. Czech J Food Sci, 23(2), 41-50.
- Çokuğraş H, Akçakaya N, 1991: Besin Allerjileri, “Temel Allerji”. V. Ulusal Allerji Kongresi, 203-213, Ankara.
- Çolak H, Aydin A, Nazli B, Ergun O, 2006: Detection of presence of cow’s milk in sheep’s cheeses by immunochromatography. Food Control, 17(11), 905-908.
- De la Fuente MA, Juárez M, 2005: Authenticity assessment of dairy products. Crit Rev Food Sci, 45, 563-585.
- Durand MM, Meusnier M, Delahaye J, Prunet P, 1974: Detection de l'addition frauduleuse de lait de vache dans les laits de chevre et de brebis par la metode de l'immunodiffusion en gelose. Bull Acad Vet, 47, 247-258.
- Elbertzhagen H, 1987: Detection of bovine casein in sheep and goat milk cheese by means of immunoelectrophoresis. Z Lebensm Unters Forsch, 185, 357-361.
- Ferreira I, Caçote H, 2003: Detection and quantification of bovine, ovine and caprine milk percentages in protected denomination of origin cheeses byreversed-phase high-performance liquid chromatography of beta-lactoglobulins. J Chromatogr A, 1015, 111-118.
- Gombocz E, Hellwing E, Petuely F, 1981: Immunological estimation of bovine casein in sheep cheese products. Z Lebensm Unters Forsch 172, 178-181.
- Iverson JL, Sheppard J, 1989: Detection of adulteration in cow, goat, and sheep cheeses utilizing gas-liquid chromatographic fatty acid data. J Dairy Sci, 72, 1707-1712.
- Karaman AD, 2007: Yağı azaltılmış beyaz peynir üretimi ve özelliklerine homojenizasyonun etkisi. Doktora tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü, İzmir.
- Klotz A, Einspanier R, 2001: Development of a DNA based screening method to detect cow milk in ewe, goat and buffalo milk and dairy products using PCR-LCREIA-technique. Milchwissenschaft, 56, 67-70.
- Koşum A, 2013: Hayvansal ürünlerin üretiminde besleme ve helallik. Journal of Islamic Law Studies, 22, 11-18.
- Krause I, Belitz HD, Kaiser KP, 1982: Detection of cow's milk in sheep's and goat's milk and cheese by isoelectric focusing on thin layers of polyacrylamide gels containing urea. Z Lenbensm Unters Forsch, 174, 195-199.
- Pierre A, Portmann A, 1970: Emploi de l'electrophorese en gel de polyacrylamide pour mettre en evidence et doser le lait de vache ajoute au lait de chevre. Application au cas de fromages. Ann Technol Agr, 19, 107-130.
- Prager MJ, 1989: Differential characteristics of fatty acids in cheese from milk of various animal species by capillary gas chromatography. J Assoc Off Anal Chem, 72, 418-421.
- Ramos M, Martinez-Castro I, Juarez M, 1977: Detection of cow's milk in manchego cheese. J Dairy Sci, 60, 87-877.
- Ramos M, Sanchez RM, Olano A, 1985: Studies on quantitative determination of caseins using polyacrylamide gel electrophoresis. Chem Mikrobiol Technol Lebensm, 9, 24-27.
- Rispoli S, Saugues R, 1989: Isoelectrofocalisation des lactoserum de fromages de melange brebis-vache sur gel de polyacrylamide. Application a la recherche et au dosage du lait de vache dans les fromages de brebis. Lait, 69, 211-222.
- Rodríguez E, Martín R, García T, Hernández PE, Sanz B, 1990: Detection of cows' milk in ewes' milk and cheese by an indirect enzyme-linked immunosorbent assay (ELISA). J Dairy Res, 57(2), 197-205.
- Song H, Xue H, Han Y, 2011: Detection of cow’s milk in Shaanxi goat’s milk with an ELISA assay. Food Control, 22(6), 883-887.
- Şimşek B, Uraz T, 2008: Koyun ve inek sütü karışımından yapılan beyaz peynirlerde süt türü oranının belirlenmesi. Gıda Dergisi, 33(2), 75-82.
- Zeleňáková L, Žıdek R, Čanıgová M, 2010: Reliability of cow casein quantitation in sheep milk and cheese by ELISA method. Journal of Food Physics, 23, 22-26.
Detection of Cow’s Milk in Ovine Cheese Consumed in Sanliurfa Province
Yıl 2017,
Cilt: 6 Sayı: 2, 163 - 166, 27.12.2017
Serap Kılıç Altun
,
Hisamettin Durmaz
Öz
Ovine cheese is a preferred type of cheese in our
country. Because ovine milk is more expensive and difficult to obtain, cow milk
is added in the production of ovine cheese. The aim of this study was to
determine the presence of cow’s milk by ELISA method in 90 ovine cheese samples
including 45 packaged and 45 unpackaged samples which are offered for retail
sale in Sanliurfa province. The proportion of cow's milk contained in ovine
cheese samples was classified as below 0.1%, below 1% and over 1% according to
the ELISA method. In 7 (7.7%) of 90 ovine cheese samples, it was below 0.1%, 53
(58.8%) in sample below 1% and in 30 (33.3%) over 1% cow milk was detected. To
prevent adulteration in milk and dairy products, analytical procedures that can
protect consumers from misleading labeling should be widespread.
Kaynakça
- Addeo F, Moio L, Chianese L, Nota G, 1989: Evaluation of bovine and water buffalo milk in mixtures of liquid milk and Mozzarella cheese by gel isoelectric focusing. Italian J Food Sci, 1, 71-80.
- Anonim, 2001: European Commission, 2001: EC 213/2001 Methods forthe analysis and quality evaluation of milk and milkproducts. Official Journal of the European Communities, 44, L37/1-L37/99.
- Borkova M, Snaselova J, 2005: Possibilities of different animal milk detection in milk and dairy products–a review. Czech J Food Sci, 23(2), 41-50.
- Çokuğraş H, Akçakaya N, 1991: Besin Allerjileri, “Temel Allerji”. V. Ulusal Allerji Kongresi, 203-213, Ankara.
- Çolak H, Aydin A, Nazli B, Ergun O, 2006: Detection of presence of cow’s milk in sheep’s cheeses by immunochromatography. Food Control, 17(11), 905-908.
- De la Fuente MA, Juárez M, 2005: Authenticity assessment of dairy products. Crit Rev Food Sci, 45, 563-585.
- Durand MM, Meusnier M, Delahaye J, Prunet P, 1974: Detection de l'addition frauduleuse de lait de vache dans les laits de chevre et de brebis par la metode de l'immunodiffusion en gelose. Bull Acad Vet, 47, 247-258.
- Elbertzhagen H, 1987: Detection of bovine casein in sheep and goat milk cheese by means of immunoelectrophoresis. Z Lebensm Unters Forsch, 185, 357-361.
- Ferreira I, Caçote H, 2003: Detection and quantification of bovine, ovine and caprine milk percentages in protected denomination of origin cheeses byreversed-phase high-performance liquid chromatography of beta-lactoglobulins. J Chromatogr A, 1015, 111-118.
- Gombocz E, Hellwing E, Petuely F, 1981: Immunological estimation of bovine casein in sheep cheese products. Z Lebensm Unters Forsch 172, 178-181.
- Iverson JL, Sheppard J, 1989: Detection of adulteration in cow, goat, and sheep cheeses utilizing gas-liquid chromatographic fatty acid data. J Dairy Sci, 72, 1707-1712.
- Karaman AD, 2007: Yağı azaltılmış beyaz peynir üretimi ve özelliklerine homojenizasyonun etkisi. Doktora tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü, İzmir.
- Klotz A, Einspanier R, 2001: Development of a DNA based screening method to detect cow milk in ewe, goat and buffalo milk and dairy products using PCR-LCREIA-technique. Milchwissenschaft, 56, 67-70.
- Koşum A, 2013: Hayvansal ürünlerin üretiminde besleme ve helallik. Journal of Islamic Law Studies, 22, 11-18.
- Krause I, Belitz HD, Kaiser KP, 1982: Detection of cow's milk in sheep's and goat's milk and cheese by isoelectric focusing on thin layers of polyacrylamide gels containing urea. Z Lenbensm Unters Forsch, 174, 195-199.
- Pierre A, Portmann A, 1970: Emploi de l'electrophorese en gel de polyacrylamide pour mettre en evidence et doser le lait de vache ajoute au lait de chevre. Application au cas de fromages. Ann Technol Agr, 19, 107-130.
- Prager MJ, 1989: Differential characteristics of fatty acids in cheese from milk of various animal species by capillary gas chromatography. J Assoc Off Anal Chem, 72, 418-421.
- Ramos M, Martinez-Castro I, Juarez M, 1977: Detection of cow's milk in manchego cheese. J Dairy Sci, 60, 87-877.
- Ramos M, Sanchez RM, Olano A, 1985: Studies on quantitative determination of caseins using polyacrylamide gel electrophoresis. Chem Mikrobiol Technol Lebensm, 9, 24-27.
- Rispoli S, Saugues R, 1989: Isoelectrofocalisation des lactoserum de fromages de melange brebis-vache sur gel de polyacrylamide. Application a la recherche et au dosage du lait de vache dans les fromages de brebis. Lait, 69, 211-222.
- Rodríguez E, Martín R, García T, Hernández PE, Sanz B, 1990: Detection of cows' milk in ewes' milk and cheese by an indirect enzyme-linked immunosorbent assay (ELISA). J Dairy Res, 57(2), 197-205.
- Song H, Xue H, Han Y, 2011: Detection of cow’s milk in Shaanxi goat’s milk with an ELISA assay. Food Control, 22(6), 883-887.
- Şimşek B, Uraz T, 2008: Koyun ve inek sütü karışımından yapılan beyaz peynirlerde süt türü oranının belirlenmesi. Gıda Dergisi, 33(2), 75-82.
- Zeleňáková L, Žıdek R, Čanıgová M, 2010: Reliability of cow casein quantitation in sheep milk and cheese by ELISA method. Journal of Food Physics, 23, 22-26.