Analysis of Vitamin D Receptor Fok1 (rs2228570) and Bsm1 (rs1544410) Polymorphism in Turkish Athletes

Öz

Aim: Vitamin D is an important molecule responsible for the development, contraction and regeneration of muscles. In this study, we aimed to research the distribution of vitamin D receptor (VDR) Fok1 (rs 2228570) and Bsm1 (rs1544410) polymorphisms in professional Turkish athletes.

Method: A total 27 athletes including 13 female and 14 male athletes participated in the study. Following DNA isolation, polymorphisms of VDR were determined by real-time PCR (RT-PCR).

Findings: 12 of the athletes have CC, 5 of them have CT and 10 of them have TT genotypes in terms of Fok1 polymorphism. 6 female athletes were determined as CC, 2 of them as CT and 5 of them as TT genotype. 6 male athletes were found as CC, 3 of them as CT and 5 of them as TT genotype. For Bsm1 polymorphism, 9 athletes have AA, 14 of athletes have AG and 4 of them have GG genotypes. 3 of the female athletes have AA, 8 of them have AG and 2 of them have GG genotypes. 6 of male athletes have AA, 6 of them have AG and 2 of them have GG genotypes.

Conclusion: In our study, it was observed that CC genotype and C allele for Fok1 polymorphism were dominant of AG genotype and A allele in Bsm 1. We believe that this study will be a reference for new studies to research the effect of VDR gene on athletic performance.

Anahtar Kelimeler

• Vitamin D receptor,athletes,sports genetic,polymorphism

Türk Atletlerde D Vitamini Reseptör Geni Fok1 (rs2228570) ve Bsm1 (rs1544410) Polimorfizmlerinin Analizi

Öz

Amaç: D vitamini kasların gelişmesinde, kasılmasında ve rejenerasyonunda görev alan önemli bir moleküldür. Çalışmamızda profesyonel Türk atletlerde D vitamin reseptör (VDR) Fok1 (rs 2228570) ve Bsm1 (rs1544410) polimorfizmlerinin dağılımını araştırmayı amaçladık.

Yöntem: Çalışmamıza 13 kadın ve 14 erkek atlet olmak üzere 27 sporcu katılmıştır. DNA izolasyonları sonrasında D vitamini reseptörü polimorfizmleri gerçek zamanlı PZR (RT-PCR) metodu ile saptanmıştır.

Bulgular: Fok1 polimorfizmi açısından atletlerin 12’si CC, 5’i CT ve 10’u ise TT genotipindedir. Kadın atletlerin 6’sı CC, 2’si CT ve 5’i ise TT genotipinde bulunmuştur. Erkek atletlerde 6 sporcu CC, 3 sporcu CT ve 5 sporcu ise TT genotipindedir. Bsm1 polimorfizmi için 9 atlet AA, 14'ü AG, 4’ü ise GG genotipindedir. Kadın atletlerin 3’ü AA, 8’i AG ve 2’si GG genotipindedir. Erkek atletlerin 6’sı AA, 6’sı AG ve 2’si GG genotipinde saptanmıştır.

Sonuç: Çalışma grubumuzda Fok1 polimorfizmi için CC genotipi ve C allelinin, Bsm1 içinde AG genotipinin ve A allelinin baskın olduğu gözlenmiştir. Bu çalışmanın VDR geninin atletik performansa etkisinin araştırılması için yapılacak yeni çalışmalar için referans olacağını düşünmekteyiz.

Anahtar Kelimeler

• D vitamini reseptörü,sporcular,spor genetiği,polimorfizm

Kaynakça

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