Effects of L-Tryptophan and Melatonin on Seed Germination Performance of Radish and Spinach in Low and High Temperature Conditions

Abstract

The aim of this study was to evaluate the effects of L-Tryptophan and Melatonin treatments on germination of radish and spinach seeds under different temperature conditions. Treated with different concentrations of L-Tryptophan (125, 250, 375 ppm) and Melatonin (5, 10, 25 µM) seeds were subjected to germination tests at optimum (21 ˚C) chilling stress (7 ˚C) and high temperature (35 ˚C) conditions. Treated with distilled water seeds were considered as control. The tested hormones had significant effects on the average germination time; the time to reach 50% germination rate; and the fresh weight results of the radish seeds at low temperature (7 ˚C) condition. At this temperature, the treatment of 10 µM melatonin prolonged the mean germination time and the time to reach 50% germination rate of the seeds compared to the hormone untreated seeds. 125 ppm and 250 ppm L-Tryptophan treatments increased the fresh weight values. The greatest effect of the hormone treatment in spinach seeds was observed in the time to reach 50% germination rate at low temperature condition (7 ˚C). The 375 ppm dose of L-Tryptophan shortened this it as compared to the control, while 25 µM melatonin treatments prolonged this period.  

Keywords

• L-Tryptophan,Melatonin,Germination,Radish,Spinach

L-Triptofan ve Melatonin’in Düşük ve Yüksek Sıcaklık Koşullarında Turp ve Ispanağın Tohum Çimlenme Performansına Etkileri

Öz

Bu çalışmanın amacı L-Triptofan ve Melatonin uygulamalarının farklı sıcaklık koşullarında turp ve ıspanak tohumlarının çimlenme özellikleri üzerindeki etkilerini araştırmaktır. Farklı konsantrasyonlarda L-Triptofan (125, 250, 375 ppm) ve Melatonin (5, 10, 25 µM) ile muamele edilmiş tohumlar, optimum (21 ˚C), üşüme stresi (7˚C) ve yüksek sıcaklık (35 ˚C) koşullarında çimlenme testlerine tabi tutulmuştur. Distile su ile muamele edilmiş tohumlar kontrol olarak kabul edilmiştir. Denenen hormonlar, düşük sıcaklıkta (7 ˚C) turp tohumlarının ortalama çimlenme süresi, %50 çimlenme oranına ulaşma süresi ve taze ağırlık sonuçları üzerine önemli etkiler oluşturmuştur. Bu sıcaklık derecesinde, 10 µM melatonin uygulaması, ortalama çimlenme süresini ve tohumların %50 çimlenme oranına ulaşma süresini, hormon uygulanmamış tohumlara göre uzatmıştır. 125 ppm ve 250 ppm L-Triptofan uygulaması yaş ağırlık değerlerini artırmıştır. Ispanak tohumlarında düşük sıcaklık koşulunda (7 ˚C) hormon uygulamalarının en büyük etkisi %50 çimlenmeye ulaşmak için gerekli süre özelliğinde gözlenmiştir. L-Triptofan' ın 375 ppm dozu bu süreyi kontrole göre kısaltırken, 25 µM melatonin uygulaması ise uzatmıştır.

Anahtar Kelimeler

• L-Triptofan,Melatonin,Çimlenme,Turp,Ispanak

Kaynakça

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