The Effects of Jujube (Zizyphus jujuba Mill.) Leaf Extract on Small Intestinal Microflora and Some Blood Parameters in Laying Hens

Öz

This study was designed to determine the effects of jujube leaf extract (JLE) added to the diets of laying hens on some blood parameters and jejunum bacterial content in laying hens (Nick Brown; 32 weeks-old). In this study, a total of 96 Nick Brown layers, 32 weeks of age, were divided into 4 groups, one of which was control (JLE-0) and 3 were experimental groups. During the ten-week experimental period, the first week being the adaptation period, the control group (JLE-0) was fed with basal feed. For each 1 kg feed, 1 g leaf; 2 g leaves; and 3 g leaves were extracted, and the treatment groups were fed with feed added to the basal feed at a level of 45 mg kg^-1(JLE-1), 90 mg kg^-1(JLE-2) and 135 mg kg^-1(JLE-3). It was found that there were no statistically significant differences between the groups in terms of total coliform bacteria counts in the jejunum. Lactobacilli counts were determined statistically significant differences between the groups (p<0.05). It was determined that two jujube leaf extract groups (JLE-2 and JLE-3) increased the lactobacilli counts in the jejunum compared to JLE-0 and JLE-1 groups (p<0.05).  It was found that jujube leaf extract had no significant effect on serum glucose, albumin, phosphorus levels and AST and GGT enzyme activities. There was a significant difference between the groups in terms of serum triglyceride levels (p<0.05). As a result, it was concluded that the use of jujube leaf extract as a feed additive in the diets at 90 mg kg^-1and 135 mg kg^-1in laying hens may partially improve the intestinal flora.

Anahtar Kelimeler

• Laying hen,jujube leaf extract,intestinal microflora,blood parameters

Yumurtacı Tavuklarda Hünnap (Zizyphus jujuba Mill.) Yaprak Ekstraktının İnce Bağırsak Mikroflorası ve Bazı Kan Parametreleri Üzerine Etkileri

Öz

Bu çalışmada, yumurtacı tavuk (Nick Brown; 32 haftalık) rasyonlarına ilave edilen hünnap yaprak ekstraktının (HYE) bazı kan parametreleri ve jejunum bakteri içeriği üzerine etkileri araştırılmıştır. Çalışmada 32 haftalık 96 adet Nick Brown yumurta tavuğu biri kontrol (HYE-0) ve 3’ü deneme grubu olacak şekilde 4 gruba ayrılmıştır. Bir haftası alıştırma periyodu olmak üzere toplam 10 hafta süren çalışmada HYE-0 grubu bazal yem ile beslenmiştir. Her 1 kg karma yem için 1 g yaprak; 2 g yaprak; 3 g yaprak kullanılarak ekstraksiyon işlemi yapılmış ve elde edilen ekstraktlar 45 mg kg^-1 (HYE-1), 90 mg kg^-1 (HYE-2) ve 135 mg kg^-1 (HYE-3) düzeylerinde bazal yeme ilave edilerek deneme grupları beslenmiştir. Çalışmada hünnap yaprak ekstraktının jejunum toplam koliform bakteri sayısı üzerine önemli bir etkisinin olmadığı; laktobasil sayısı üzerine ise önemli bir etkisinin olduğu (p<0.05) tespit edilmiştir. Hünnap yaprak ekstraktının rasyondaki 90 mg kg^-1 ve 135 mg kg^-1 düzeylerinin laktobasil sayısını arttırdığı belirlenmiştir (p<0.05). Hünnap yaprak ekstraktının serum glukoz, albümin, fosfor düzeylerine ve AST ile GGT enzim aktiviteleri üzerine önemli bir etkisinin olmadığı tespit edilmiştir. Serum trigliserid düzeyi bakımından ise gruplar arasında önemli bir fark olmuştur (p<0.05). Sonuç olarak, hünnap yaprak ekstraktının yumurtacı tavuk rasyonlarında 90 mg kg^-1 ve 135 mg kg^-1 düzeylerinde kullanılmasının bağırsak florasını kısmen iyileştirebileceği kanaatine varılmıştır.

Anahtar Kelimeler

• Yumurtacı tavuk,hünnap yaprak ekstraktı,bağırsak mikroflorası,kan parametreleri

Kaynakça

1. Abdu, S. B., Ehoche, O. W., Adamu, A. M., Bawa, G. S., Hassan, M. R., Yashim, S. M., & Adamu, H. Y. (2012). Effect of varying levels of Zizyphus (Zizyphus mauritiana) leaf meal inclusion in concentrate diet on performance of growing Yankasa ram lambs fed maize stover basal diet. Iranian Journal of Applied Animal Science, 2(4), 323-330.
2. Abdulameer, Y. S., Husain, F., & Al-cekal, S. H. A. (2017). Efficacy of Ziziphus mauritiana leaves extract as antibiotic alternatives in broiler chicken. Journal of Entomology and Zoology Studies, 5(5), 742-746.
3. Abdulla, G., Abdel-Saime, M. A. S., & Zaki, D. (2016). Evaluation of the antioxidant and antimicrobial effects of ziziphus leaves extract in sausage cold storage. Pakistan Journal of Food Sciences, 26(1), 10-20.
4. Ahmad, B., Khan, I., Bashir, S., Azam, S., & Hussain, F. (2011). Screening of Zizyphus jujuba for antibacterial, phytotoxic and haemagglutination activities. African Journal of Biotechnology, 10(13), 2514-2519.
5. Altop, A., Erener, G., Duru, M. E., & Isik, K. (2018). Effects of essential oils from Liquidambar orientalis Mill. leaves on growth performance, carcass and some organ traits, some blood metabolites and intestinal microbiota in broilers. British Poultry Science, 59(1), 121-127.
6. AOAC, (2000). Official Methods of Analysis (17th Ed.). Association of Official Analytical Chemists, Washington, DC.
7. Asheg, A. A., El-Nyhom, S. M., Ben Naser, K. M., Kanoun, A. H., & Abouzeed, Y. M. (2014). Effect of Arbutus pavarii, Salvia officinalis and Zizyphus vulgaris on growth performance and intestinal bacterial count of broiler chickens. International Journal of Veterinary Science and Medicine, 2, 151-155.
8. Bashtani, M., Tehrani, M. H., Naserian, A. A., & Fathi, M. H. (2013). The effect of different level of Ziziphus jujube Mill foliage on feed intake, blood metabolites and milk production and composition in Fluffy Goats. Iranian Journal of Animal Science Research, 5(2), 19.

9. Bayram, Y., Torlak, Y., & Sağdıç, O. (2019). Üvez meyvesinin antioksidan aktivitesi. Avrupa Bilim ve Teknoloji Dergisi, 16, 933-939.
10. Behnamifar, A., Rahimi, S., Karimi Torshizi, M. A., Hasanpour, S., & Mohammadzade, Z. (2015). Effect of thyme, garlic and caraway herbal extracts on blood parameters, productivity, egg quality, hatchability and intestinal bacterial population of laying Japanese quail. Iranian Journal of Veterinary Medicine, 9(3), 179-187.
11. Botsoglou, N., Florou-Paneri, P., Bostoglou, E., Dotas, V., Giannenas, I., Koidis, A., & Mitrakos, P. (2005). The effect of feeding rosemary, oregano, saffron and α-tocopheryl acetate on hen performance and oxidative stability of eggs. South African Journal of Animal Science, 35(3), 143-151.
12. Bölükbaşı, Ş. C., Erhan, M., & Kaynar., Ö. (2008). The effect of feeding thyme, sage and rosemary oil on laying hen performance, cholesterol and some proteins ratio of egg yolk and Escherichia coli count in feces. Archive fur Geflugelkunde, 72(5), 231-237.
13. Bulbul, T., Ozdemir, V., & Bulbul, A. (2015). Use of sage (Salvia triloba L.) and laurel (Laurus nobilis L.) oils in quail diets. Eurasian Journal of Veterinary Sciences, 31(2), 95-101. Carpenter, K.J., & Clegg, K.M. (1956). The metabolizable energy of poultry feeding stuffs in relation to their chemical composition. Journal of the Science of Food and Agriculture, 7(1), 45-51.
14. Cayan, H., & Erener, G. (2015). Effect of olive leaf (Olea europaea) powder on laying hens performance, egg quality and egg yolk cholesterol levels. Asian Australasian Journal of Animal Sciences, 28(4), 538-543.
15. Cheng, G., Hao, H., Xie, S., Wang, X., Dai, M., Huang, L., & Yuan, Z. (2014). Antibiotic alternatives: the substitution of antibiotics in animal husbandry? Frontiers in Microbiology, 5, 1-15.
16. Christaki, E. V., Bonos, E. M., & Florou-Paneri, P. C. (2011). Comparative evaluation of dietary oregano, anise and olive leaves in laying Japanese quails. Brezilian Journal of Poultry Science, 13(2), 97-101.
17. Christaki, E., Bonos, E., Giannenas, I., & Florou-Paneri, P. (2012). Aromatic plants as a source of bioactive compounds. Agriculture, 2, 228-243.
18. Çiftçi, M., Güler, T., Dalkiliç, B., & Ertas, O. N. (2005). The effect of anise oil (Pimpinella anisum L.) on broiler performance. International Journal of Poultry Science, 4(11), 851-855.
19. Çimrin, T., & Demirel, M. (2016). Yumurtacı tavuklarda biberiye (Rosmarinus officinalis L.) uçucu yağının bazı kan parametreleri ve ince bağırsak mikroflorası üzerine etkileri. Türk Tarım-Gıda Bilim ve Teknolojisi Dergisi, 4(9), 769-775.
20. Dalkılıç, B. (2007). Karanfil ekstraktının broylerlerde performans, ham besin maddelerinin sindirilme derecesi, sindirim organları ağırlığı ve bağırsaklardaki toplam koliform bakteri sayısı üzerine etkisi. Doktora Tezi, Fırat Üniversitesi Sağlık Bilimleri Enstitüsü, Elazığ.
21. Demir, E., Sarica, Ş., Özcan, M. A., & Suiçmez, M. (2005). The use of natural feed additives as alternatives to an antibiotic growth promoter in broiler diets. Archiv fur Geflugelkunde, 69(3), 110-116.
22. Demir, E., Kilinc, K., Yildirim, Y., Dincer, F., & Eseceli, H. (2008). Comparative effects of mint, sage, thyme and flavomycin in wheat-based broiler diets. Archiva Zootechnica, 11(3), 54-63.
23. El-Maaty, H. M. A., El-Khateeb, A. Y., El-Naggar, A. S., Sherif, S. K., & El-Said, E. A. (2018). Performance of hybrid chickens as influenced by phytogenic extracted meal-supplemented diet as antibiotic alternatives during summer. Journal of Agricultural Science, 10(7), 471-484.
24. Eratalar, S. A., Okur, N., Yaman, A., & Karadeniz, T. (2017). Bıldırcın yemlerinde iç ceviz, ceviz yaş kabuğu ve yaprağı kullanımının yumurta kabuk ve yumurta sarısı rengine etkileri. Bahçe, 46(Özel Sayı 2), 71-76.
25. Gavris, T., Arabela, U., & Saracila, M. (2019). Use of phyto-additives in poultry nutrition. Scientific Papers: Animal Science and Biotechnologies, 52(1), 13-18.
26. Gheisar, M. M., & Kim, I. H. (2018). Phytobiotics in poultry and swine nutrition -a review. Italian Journal of Animal Science, 17(1), 92-99.
27. Goyal, R., Sharma, P. L., & Singh, M. (2011). Possible attenuation of nitric oxide expression in anti-inflammatory effect of Ziziphus jujuba in rat. Journal of Natural Medicines, 65, 514-518.
28. Grashorn, M. A. (2010). Use of phytobiotics in broiler nutrition -an alternative to infeed antibiotics? Journal of Animal and Feed Sciences, 19, 338-347.
29. Gündoğmuş, M. E., & Taşçı, M. (2017). Hünnap (Zizyphus jujube mill.) bahçelerinde gelir yöntemine göre değerleme: Denizli ili Çivril ilçesi örneği. Tekirdağ Ziraat Fakültesi Dergisi, 14(2), 42-53.
30. Hürkan, Y. K. (2019). Hünnap (Ziziphus jujuba Mill.) meyvesi: Geçmişten günümüze tıbbi önemi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 9(3), 1271-1281.
31. IBM Corp. (2011). IBM SPSS Statistics for Windows, Version 20.0. IBM Corporation, Armonk, NY.
32. Inusa, S. K. (2012). Effect of feeding graded levels of Ziziphus mauritiana leaf on blood parameters of growing rabbits. Proceedings of the 46th Annual Conference of the Agricultural Society of Nigeria, Nigeria.
33. Jang, I. S., Ko, Y. H., Kang, S. Y., & Lee, C. Y. (2007). Effect of a commercial essential oil on growth performance, digestive enzyme activity and intestinal microflora population in broiler chickens. Animal Feed Science and Technology, 134, 304-315.
34. Kahkönen M. P., Hopia, A. I., Vuorela, H. J., Rauha, J. P., Pihlaja, K., Kujala, T. S., & Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry, 47, 3954-3962.
35. Karasu, K., & Öztürk, E. (2014). Tıbbi ve aromatik bitkilerin kanatlılarda antioksidan ve antimikrobiyal etkileri. Türk Tarım ve Doğa Bilimleri Dergisi, Özel Sayı-2, 1766-1772).
36. Khan, S. H., Anjum M. A., Parveen, A., Khawaja, T., & Ashraf, N. M. (2013). Effects of black cumin seed (Nigella sativa L.) on performance and immune system in newly evolved crossbred laying hens. Veterinary Quarterly, 33(1), 13-19.
37. Khan, R. U., Chand, N., & Ali, A. (2016). Effect of organic acids on the performance of japanese Quails. Pakistan Journal of Zoology, 48(6), 1799-1803.
38. Kırkpınar, F., & Açıkgöz, Z. (2003). Kanatlı hayvanlarda nişasta tabiatında olmayan polisakkaritlerin sindirim sistemi mikroflorası üzerine etkileri. Hayvansal Üretim, 44(2), 20-28. Ma, K., Zhao, G., & Liu, Y. (2014). Effects of jujube powder on laying performance and egg quality in hens. China Feed, 19.
39. Ma, K., Zhao, Y., & Yanci, L. (2017). Effects of jujube powder on lipid metabolism, antioxidant ability and immune function in laying hens. China Feed, 19, 2.
40. Muthusamy, N., & Sankar, V. (2015). Phytogenic compounds used as a feed additives in poultry production. International Journal of Science, Environment and Technology, 4(1), 167-171.
41. Najafi, S. (2013). Phytochemical screening and antibacterial activity of leaf extract of Ziziphus mauritiana Lam. International Research Journal of Applied and Basic Sciences, 4(10), 3274-3276.
42. Ocak, N., Erener, G., Ak, F. B., Sungu, M., Altop, A., & Özmen, A. (2008). Performance of broilers fed diets supplemented with dry peppermint (Mentha piperite L.) or thyme (Thymus vulgaris L.) leaves as growth promoter source. Czech Journal of Animal Science, 53(4), 169-175.
43. Priyanka, C., Kumar, P., Bankar, S. P., & Karthik, L. (2015). In vitro antibacterial activity and gas chromatography-mass spectroscopy analysis of Acacia karoo and Ziziphus mauritiana extracts. Journal of Taibah University for Science, 9, 13-19.
44. Rahimi, S., Teymouri Z. Z., Karimi T. M., Omidbaigi, R., & Rokni, H. (2011). Effect of the three herbal extracts on growth performance, immune system, blood factors and intestinal selected bacterial population in broiler chickens. Journal of Agricultural Science and Technology, 13, 527-539.
45. San, B., & Yildirim, A. N. (2010). Phenolic, alpha-tocopherol, beta-carotene and fatty acid composition of four promising jujube (Zizyphus jujuba Miller) selections. Journal of Food Composition and Analysis, 23, 706-710.
46. Savoia, D. (2012). Plant-derived antimicrobial compounds: alternatives to antibiotics. Future Microbiology, 7(8), 979-990.
47. Soltan, M. A., Shewita, R. S., & El-Katcha, M. I. (2008). Effect of dietary anise seeds supplementation on growth performance, immune response, carcass traits and some blood parameters of broiler chickens. International Journal of Poultry Sience, 7(11), 1078-1088.
48. Son, J. H. (2014). Effects of dietary Zizyphus jujuba seed meal on broiler performance. Korean Journal of Poultry Science, 41(4), 279-285.
49. Şimşek, Ü. G., Çiftçi, M., Özçelik, M., Azman, M. A., Tonbak, F., & Özhan, N. (2015). Effects of cinnamon and rosemary oils on egg production, egg quality, hatchability traits and blood serum mineral contents in laying quails (Coturnix coturnix Japonica). Ankara Üniversitesi Veteriner Fakültesi Dergisi, 62, 229-236.
50. Thayalini, K., Shanmugavelu, S., Saminathan, P. M., Siti Masidayu, M. S., Nor Idayusni, Y., Zainuddin, H., Akmal, N. C. A., & Wong, H. K. (2011). Effects of Cymbopogon citratus leaf and Zingiber officinale rhizome supplementation on growth performance, ileal morphology and lactic acid concentration in broilers. Malaysian Journal of Animal Science, 14, 43-49.
51. Torki, M., Sedgh-Gooya, S., & Mohammadi, H. (2018). Effects of adding essential oils of rosemary, dill and chicory extract to diets on performance, egg quality and some blood parameters of laying hens subjected to heat stress. Journal of Applied Animal Research, 46(1), 1118-1126.
52. Wallace, P. R. J., Oleszek, W., Franz, C., Hahn, I., Baser, K. H. C., Mathe, A., & Teichman, K. (2010). Dietary plant bioactives for poultry health and productivity. British Poultry Science, 51(4), 461-487.
53. Windisch, W., Schedle, K., Plitzner, C., & Kroismayr, A. (2008). Use of phytogenic products as feed additives for swine and poultry. Journal Animal of Science, 86, 140-148.
54. Yalçın, S., Yalçın, S., Erol, H., Buğdaycı, K. E., Özsoy, B., & Çakır, S. (2009). Effects of dietary black cumin seed (Nigella sativa L.) on performance, egg traits, egg cholesterol content and egg yolk fatty acid composition in laying hens. Journal of the Science of Food and Agriculture, 89: 1737-1742.
55. Yalçın, S., Yalçın, S., Uzunoğlu, K., Duyum, H. M., & Eltan, Ö. (2012). Effects of dietary yeast autolysate (Saccharomyces cerevisiae) and black cumin seed (Nigella sativa L.) on performance, egg traits, some blood characteristics and antibody production of laying hens. Livestock Science, 145: 13-20.
56. Yang, Y., Iji, P. A., & Choct, M. (2009). Dietary modulation of gut microflora in broiler chickens: a review of the role of six kinds of alternatives to in-feed antibiotics. World’s Poultry Science Association, 65, 97-114.
57. Yaşa, H. (2019). Türkiye Çanakkale’den hünnap meyvesinin (Zizyphus jujuba.) sulu ekstresinin toplam fenolik miktarı ve antioksidan aktivitesi. Iğdır Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 9(1), 158-168.
58. Yeşilbağ, D. (2007). Fitobiyotikler. Uludağ Üniversitesi Veteriner Fakültesi Dergisi, 26(1-2), 33-39.
59. Zhonghua, W., Huang, X., & Quanhai, G. (2011). Effects of jujube oligosaccarides on production performance and immune function of laying chicken. China Feed, 19.
60. Zhu, W., Li, D., Wang, J., Wu, H., Xia, X., Bi, W., Guan, H., & Zhang, L. (2015). Effects of polymannuronate on performance, antioxidant capacity, immune status, cecal microflora, and volatile fatty acids in broiler chickens. Poultry Science, 94, 345-352.