Yıl 2019, Cilt 5 , Sayı 1, Sayfalar 90 - 95 2019-06-30

Heterodera latipons’un SCAR (Sequence Characterised Amplified Region) Primer Kullanılarak Tanımlanması
Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers

Mustafa İmren [1] , Abdurrahman Sami Koca [2] , Abdelfattah Amer Dababat [3]


Akdeniz Tahıl kist nematodu Heterodera latipons, mücadelesinin yapılmadığı buğday üretim alanlarında önemli derece ürün kayıplarına neden olabilebilmektedir. Nematodlar ile mücadelede sürüdürülebilir ve etkili bir mücadele için nematodun türünün bilinmesi oldukça önemlidir. Bu çalışmada, Doğu Akdeniz ve Güney Doğu Anadolu bölgeleri tahıl alanlarından elde edilen 25 adet Heterodera latipons popülasyonu, türe spesifik primer çifti (SCAR) H-latacF ve H-latactR kullanılarak tanımlanmıştır. Çalışma sonucunda, Heterodera latipons popülasyonlarının tanımlanmasında H-latacF ve H-latactR primerleri etmeni başarılı bir şekilde tanımlamış olup, nematodun Doğu Akdeniz ve Güney Doğu Anadolu bölgelerindeki en önemli Tahıl kisti nematod türü olduğu ortaya konulmuştur. Ayrıca, çalışmada kullanılan türe özgü primerler Heterodera latipons popülasyonlarının kolay ve hızlı tanımlanmasında kullanılabileceği sonucuna varılmıştır. 
The Mediterranaen cereal cyst nematode, Heterodera latipons, causing serious yield losses in protected cultivation cereal cropping areas in Turkey. An accurate and reliable identification is primarily needed to establish effective, sustainable and environmentally safe control measures to any cyst nematode species. In this study, twenty-five populations of Heterodera latipons collected from cereal areas in East-Mediterranean and South-East Anatolian regions were identified using species-specific primers (SCAR). The SCAR primer pair, H-latac F and H-latact R, defined species-specific primers were used to identify Heterodera populations. In this study stated that H-latac F and H-latact R primers for identifying of H. latipons can be efficient tools to identify the Turkish Mediterranean cereal cyst nematode populations. Moreover, the results indicated that H. latipons was the prominent cereal cyst nematode species in the East Mediterranean and South East Anatolian regions of Turkey. This clarified approach may supply a quick basis for identification of H. latipons populations for their future management and facilitate checking its distribution in the agricultural areas.
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Konular Fen
Bölüm Bitki Koruma
Yazarlar

Orcid: 0000-0002-7217-9092
Yazar: Mustafa İmren (Sorumlu Yazar)
Ülke: Turkey


Orcid: 0000-0002-7657-5615
Yazar: Abdurrahman Sami Koca
Ülke: Turkey


Orcid: 0000-0002-3172-0452
Yazar: Abdelfattah Amer Dababat
Kurum: International Maize and Wheat Improvement Center

Tarihler

Yayımlanma Tarihi : 30 Haziran 2019

Bibtex @araştırma makalesi { ijaws512967, journal = {International Journal of Agricultural and Wildlife Sciences}, issn = {}, eissn = {2149-8245}, address = {}, publisher = {Bolu Abant İzzet Baysal Üniversitesi}, year = {2019}, volume = {5}, pages = {90 - 95}, doi = {10.24180/ijaws.512967}, title = {Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers}, key = {cite}, author = {İmren, Mustafa and Koca, Abdurrahman Sami and Dababat, Abdelfattah Amer} }
APA İmren, M , Koca, A , Dababat, A . (2019). Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers. International Journal of Agricultural and Wildlife Sciences , 5 (1) , 90-95 . DOI: 10.24180/ijaws.512967
MLA İmren, M , Koca, A , Dababat, A . "Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers". International Journal of Agricultural and Wildlife Sciences 5 (2019 ): 90-95 <https://dergipark.org.tr/tr/pub/ijaws/issue/43026/512967>
Chicago İmren, M , Koca, A , Dababat, A . "Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers". International Journal of Agricultural and Wildlife Sciences 5 (2019 ): 90-95
RIS TY - JOUR T1 - Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers AU - Mustafa İmren , Abdurrahman Sami Koca , Abdelfattah Amer Dababat Y1 - 2019 PY - 2019 N1 - doi: 10.24180/ijaws.512967 DO - 10.24180/ijaws.512967 T2 - International Journal of Agricultural and Wildlife Sciences JF - Journal JO - JOR SP - 90 EP - 95 VL - 5 IS - 1 SN - -2149-8245 M3 - doi: 10.24180/ijaws.512967 UR - https://doi.org/10.24180/ijaws.512967 Y2 - 2019 ER -
EndNote %0 Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers %A Mustafa İmren , Abdurrahman Sami Koca , Abdelfattah Amer Dababat %T Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers %D 2019 %J International Journal of Agricultural and Wildlife Sciences %P -2149-8245 %V 5 %N 1 %R doi: 10.24180/ijaws.512967 %U 10.24180/ijaws.512967
ISNAD İmren, Mustafa , Koca, Abdurrahman Sami , Dababat, Abdelfattah Amer . "Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers". International Journal of Agricultural and Wildlife Sciences 5 / 1 (Haziran 2019): 90-95 . https://doi.org/10.24180/ijaws.512967
AMA İmren M , Koca A , Dababat A . Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers. International Journal of Agricultural and Wildlife Sciences. 2019; 5(1): 90-95.
Vancouver İmren M , Koca A , Dababat A . Identification of Heterodera latipons Using PCR with Sequence Characterised Amplified Region (SCAR) Primers. International Journal of Agricultural and Wildlife Sciences. 2019; 5(1): 95-90.