Chemical Vapor Deposition of Poly(hydroxyethyl methacrylate-glycidyl methacrylate) Thin Film Coatings for Immobilization of Human Serum Albumin

Öz

In this study, poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) (P(HEMA-GMA) thin films were deposited on crosslinked polyvinyl alcohol (PVA) supports by initiated chemical vapor deposition (iCVD). Use of the tert-butyl peroxide as an initiator allowed very high deposition rates up to 125 nm/min at a filament temperature of 280 ^oC. FTIR and XPS analyses of the deposited films confirmed that the epoxide functionality of the copolymers increased with increasing glycidyl methacrylate fraction in the reactor inlet. The potential of the glycidyl methacrylate containing copolymer films to act as substrates for protein immobilization was revealed. The immobilization of Human Serum Albumin (HSA), which is the main protein to carry fatty acids and metals to target tissues, was carried out via solid phase extraction. The effects of film composition and thickness on binding capacities of the protein to the polymers were studied. The maximum protein binding for the iCVD synthesized copolymer films was found to be 223 µg.cm^-2. Protein binding was also clarified by FTIR, AFM, and SEM analyses. The mild immobilization conditions, easy and rapid membrane preparation, one-step protein binding at substantially higher levels and membrane reusability make iCVD deposited   P(HEMA-GMA) films useful for biomolecules immobilization and for several biochemical processes.

Anahtar Kelimeler

• iCVD,Thin film hydrogel membrane,Protein immobilization,P(HEMA-GMA),Crosslinked PVA

Kaynakça

1. 1. Cheng, X.; Kondyurin, A.; Bao, S.; Bilek, M. M.; Ye, L. App. Surf. Sci. 2017, 416, 686.
2. 2. Kalkan, N. A.; Aksoy, S.; Aksoy, E. A.; Hasırcı, N.; Jour. App. Poly. Sci. 2011, 123, 707. 3. Liu, J.; Liang, Y.; Shen, J.; Bai, Q. Analytical and bioanalytical chemistry. 2018, 410, 573.
3. 4. Gunasekera, B.; Abou Diwan, C.; Altawallbeh, G.; Kalil, H., Maher, S.; Xu, S.; et al. ACS applied materials & interfaces. 2018, 10, 7745.
4. 5. Zhao M, Li H, Liu W, Guo Y, Chu W. Biosensors and Bioelectronics. 2016;79:581-8.
5. 6. Calvo JN-M, Elices M, Guinea GV, Pérez-Rigueiro J, Arroyo-Hernández M. Applied Surface Science. 2017;416:965-70.
6. 7. Bas SZ, Maltas E, Sennik B, Yilmaz F, Vural HC. Journal of Applied Polymer Science. 2014;131(16).
7. 8. Lei Z, Gao J, Liu X, Liu D, Wang Z. ACS applied materials & interfaces. 2016;8(16):10174-82.
8. 9. Tong Z, Schiel JE, Papastavros E, Ohnmacht CM, Smith QR, Hage DS. Journal of Chromatography A. 2011;1218(15):2065-71.

9. 10. Erol K, Cebeci BK, Köse K, Köse DA. International journal of biological macromolecules. 2019;123:738-43.
10. 11. Demir EF, Kuru CI, Uygun M, Aktaş Uygun D, Akgöl S. Journal of Biomaterials science, Polymer edition. 2018;29(4):344-59.
11. 12. Vieira AP, Pimenta AF, Silva D, Gil MH, Alves P, Coimbra P, et al. European Journal of Pharmaceutics and Biopharmaceutics. 2017;120:52-62.
12. 13. Noein L, Haddadi-Asl V, Salami-Kalajahi M. International Journal of Polymeric Materials and Polymeric Biomaterials. 2017;66(3):123-31.
13. 14. Bayramoglu G, Ozalp C, Oztekin M, Guler U, Salih B, Arica MY. Talanta. 2019;191:59-66.
14. 15. Çiçek Ç, Yılmaz F, Özgür E, Yavuz H, Denizli A. Chemosensors. 2016;4(4):21.
15. 16. Bakhshpour M, Derazshamshir A, Bereli N, Elkak A, Denizli A. Materials Science and Engineering: C. 2016;61:824-31.
16. 17. Elkak A, Hamade A, Bereli N, Armutcu C, Denizli A. Analytical biochemistry. 2017;525:1-7.
17. 18. Bayramoglu, G; Arica, M.Y; Bektas, S; Journal of Applied Polymer Science,2007; 106, 169–177.
18. 19. Zeng J, Zhang Z, Dong Z, Ren P, Li Y, Liu X. Reactive and Functional Polymers. 2017;115:1-9.
19. 20. Salehi SM, Di Profio G, Fontananova E, Nicoletta FP, Curcio E, De Filpo G. Journal of Membrane Science. 2016;504:220-9.
20. 21. Schwellenbach J, Kosiol P, Soelter B, Taft F, Villain L, Strube J. Reactive and Functional Polymers. 2016;106:32-42.
21. 22. Bayramoglu G, Senkal BF, Yilmaz M, Arica MY. Bioresource technology. 2011;102(21):9833-7.
22. 23. Ramalingam N, Rajiv S. Journal of Advanced Research in NanoScience and NanoTechnology. 2018;1(1&2):1-9.
23. 24. Kaya AST, Cengiz U. Progress in Organic Coatings. 2019;126:75-82.
24. 25. M. Gürsoy, T. Uçar, Z. Tosun, M. Karaman, Plasma Process. Polym. 13 (2016) 438–446.
25. 26. Brian J. McMahon Courtney A. Pfluger Bing Sun Katherine S. Ziemer Daniel D. Burkey Rebecca L. Carrie, Materials Research A, 102 (2014) 2375-2382.
26. 27. McMahon BJ, Pfluger CA, Sun B, Ziemer KS, Burkey DD, Carrier RL. Journal of Biomedical Materials Research Part A. 2014;102(7):2375-82.
27. 28. Alf ME, Asatekin A, Barr MC, Baxamusa SH, Chelawat H, Ozaydin‐Ince G, et al. Advanced Materials. 2010;22(18):1993-2027.
28. 29. G. Ozaydin-Ince, A.M. Coclite, K.K. Gleason, Reports on Progress in Physics, (2011) 75 016501.30. Mao Y, Gleason KK. Langmuir. 2004;20(6):2484-8.
29. 31. Ozaydin-Ince G, Gleason KK. Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films. 2009;27(5):1135-43.
30. 32. Hoffman A, Schmer G, Harris C, Kraft W. ASAIO Journal. 1972;18(1):10-6.
31. 33. Roointan A, Farzanfar J, Mohammadi-Samani S, Behzad-Behbahani A, Farjadian F. Int J Pharm. 2018;552(1-2):301-11.
32. 34. Zhao W, Yang R-J, Qian T-T, Hua X, Zhang W-B, Katiyo W. International journal of molecular sciences. 2013;14(6):12073-89.
33. 35. Arıca M.Y, Bayramoğlu G, Polyethyleneimine-grafted poly(hydroxyethyl methacrylate-co-glycidyl methacrylate) membranes for reversible glucose oxidase immobilization, Biochemical Engineering Journal. 2004, 20 (1); 73-77.
34. 36. Karaman M, Çabuk N, Thin Solid Films, Initiated chemical vapor deposition of pH responsive poly(2-diisopropylamino)ethyl methacrylate thin films, 2012;520(21):6484-6488.
35. 37. Buzoglu L, Maltas E, Ozmen M, Yildiz S. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 2014;442:139-45.
36. 38. Tenhaeff WE, Gleason KK. Initiated Chemical Vapor Deposition of Alternating Copolymers of Styrene and Maleic Anhydride, Langmuir. 2007;23(12):6624-30.
37. 39. Lin-Vien D, Colthup NB, Fateley WG, Grasselli JG. The handbook of infrared and Raman characteristic frequencies of organic molecules: Elsevier; 1991.
38. 40. Maltas E, Ozmen M, Yildirimer B, Kucukkolbasi S, Yildiz S. Interaction between ketoconazole and human serum albumin on epoxy modified magnetic nanoparticles for drug delivery. Journal of nanoscience and nanotechnology. 2013;13(10):6522-8.
39. 41. Maltas E, Ozmen M, Yildiz S, Ersoz M. Binding affinity of serum proteins to epoxy modified magnetite nanoparticles. Advanced Science Letters. 2012;17(1):143-8.
40. 42. Bayramoğlu G, Kaçar Y, Denizli A, Arıca MY. Covalent immobilization of lipase onto hydrophobic group incorporated poly (2-hydroxyethyl methacrylate) based hydrophilic membrane matrix. Journal of food engineering. 2002;52(4):367-74.
41. 43. Kong J, Yu S. Fourier transform infrared spectroscopic analysis of protein secondary structures. Acta biochimica et biophysica Sinica. 2007;39(8):549-59.
42. 44. Barth A. Infrared spectroscopy of proteins. Biochimica et Biophysica Acta (BBA)-Bioenergetics. 2007;1767(9):1073-101.
43. 45. Bayramoğlu G, Akgöl S, Bulut A, Denizli A, Arıca MY. Covalent immobilisation of invertase onto a reactive film composed of 2-hydroxyethyl methacrylate and glycidyl methacrylate: properties and application in a continuous flow system. Biochemical Engineering Journal. 2003;14(2):117-26.