Yaban Eriği (Prunus spinosa L.) Özütlerinin Antioksidan, Enzim İnhibisyonu ve Antiproliferatif Etkinliklerinin İncelenmesi

Bu makalenin ilk hali 15 Aralık 2021 tarihinde yayımlandı. https://dergipark.org.tr/tr/pub/ijlsb/article/851220

Düzeltme Notu

2021 yılı 4. Cilt 3. Sayıda yayınlanan; “Yaban Eriği (Prunus spinosa L.) Özütlerinin Antioksidan, Enzim İnhibisyonu ve Antiproliferatif Etkinliklerinin İncelenmesi”, “Investigation of Antioxidant, Enzyme Inhibition and Antiproliferative Activities of Blackthorn (Prunus spinosa L.) Extracts” başlıklı makalede yer alan; “This work was supported by Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit, Project number: FYL 2014/222 as MSc Thesis of MS.” ifadesinde proje numarası sehven yanlış yazılmıştır ve makalede sorumlu yazar tarafından hatalı olarak yazıldığı beyan edilmiştir. Yapılan bu hatadan dolayı yazarlar, okuyuculardan özür dilemektedir. Makalede yer alan hatanın giderilmesi amacıyla bu düzeltme metni sunulmuştur. Bildirilen Proje numarası düzeltilmiş hali: “This work was supported by Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit, Project number: FYL 2014/212 as MSc Thesis of MS.”

Öz

Doğal ürünler, farmakoloji ve tıpta ilaç keşfinde anahtar bir role sahiptir. Türkiye'nin batısındaki Çanakkale ilinde yetişen Prunus spinosa L. (karaçalı), tıbbi bir bitki olarak bilinir ve fenolikler, flavonoidler ve antosiyanidinler gibi biyolojik olarak aktif bileşiklerin zengin bir kaynağıdır. Bitkinin çiçek ve meyve özleri birçok çalışmanın konusudur, ancak genellikle biyoinhibisyon çalışmaları için potansiyelinin ayrıntılarından yoksundurlar. Bu nedenle, bu çalışmada bitkinin metanol, etil asetat, diklorometan ve n-hekzan özütlerinin antioksidan, enzim inhibisyonu ve antiproliferatif etkinlik çalışmalarının incelenmesi amaçlanmıştır. Etil asetat ve metanol özütleri, DPPH, FRAP, CUPRAC ve TEAC analizlerinde daha iyi antioksidan etkinlik sergiledi. Özütlerin enzim inhibisyon çalışmaları, β-laktamaz ve çeşitli proteazlar kullanılarak yapıldı. 10 mg/mL derişimdeki metanol (FL) ve etil asetat (FL ve L) özütleri, sırasıyla % 22.6, % 34.7 ve % 92.1 değerleriyle a-kimotripsin, tripsin ve papine karşı iyi inhibisyon gösterdi. Ayrıca metanol ve etil asetat özütleri, MTT testi kullanılarak sağlıklı hücrelere (HUVEC) kıyasla Hep3B ve HT29 gibi kanser hücrelerine karşı daha yüksek sitotoksik etki göstermiştir. Bulgular, P. spinosa L. özütleri ve bileşenlerinin yeni ilaç adaylarının ileri incelemeleri için potansiyel olabileceğini gösterdi.

Anahtar Kelimeler

• Prunus spinosa L
• enzim inhibisyon
• proteazlar
• tirozinaz
• sitotoksisite
• antioksidan

Destekleyen Kurum

Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit

Proje Numarası

FYL 2014/212

Teşekkür

We thank Çanakkale Onsekiz Mart University for financial support. The authors also thank Ersin Karabacak for the identification of the plant.

Investigation of antioxidant, enzyme inhibition and antiproliferative activities of blackthorn (Prunus spinosa L.) extracts

Bu makalenin ilk hali 15 Aralık 2021 tarihinde yayımlandı. https://dergipark.org.tr/tr/pub/ijlsb/article/851220

Düzeltme Notu

The text you provided seems to be a correction statement related to an article titled "Investigation of Antioxidant, Enzyme Inhibition and Antiproliferative Activities of Blackthorn (Prunus spinosa L.) Extracts," published in Volume 4, Issue 3 of the year 2021. The correction addresses an error in the project number mentioned in the article. The corrected project number is "FYL 2014/212" instead of the previously reported "FYL 2014/222." This correction is being made by the responsible author(s) to rectify the mistake and apologize to the readers for any confusion caused by the error. Reported number corrected version is: "This work was supported by Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit, Project number: FYL 2014/212 as MSc Thesis of MS."

Öz

Natural products have a key role in drug discovery in pharmacology and medicine. Prunus spinosa L. (blackthorn) grown in Çanakkale province in western Turkey, is known as a medicinal plant, a rich source of biologically active compounds such as phenolics, flavonoids and anthocyanidins. The flower and fruit extracts of the plant are subjects of many studies, but they usually lack in details of its potential for bio-inhibition studies. Thus, this study aimed to investigate the antioxidant, enzyme inhibition and antiproliferative activity studies of the methanol, ethyl acetate, dichloromethane, and n-hexane extracts of the plant. The ethyl acetate and methanol extracts demonstrated more better antioxidant activity with DPPH, FRAP, CUPRAC, and TEAC assays. Enzyme inhibition studies of the extracts were performed using β-lactamase and various proteases. The methanol (FL) and ethyl acetate (FL and L) extracts at the concentration of 10 mg/mL, showed good inhibition against α-chymotrypsin, trypsin, and papain with values of 22.6%, 34.7% and 92.1%, respectively. Furthermore, the methanol and ethyl acetate extracts have displayed higher cytotoxic effect against cancer cells such as Hep3B and HT29 when compared to healthy cells (HUVEC) using MTT assay. The findings suggest that P. spinosa L. extracts and their components may be potential for further investigations of novel drug candidates.

Anahtar Kelimeler

• Prunus spinosa L.
• enzyme inhibition
• proteases
• tyrosinase
• cytotoxicity
• antioxidant

Destekleyen Kurum

Çanakkale Onsekiz Mart University, The Scientific Research Coordination Unit

Proje Numarası

FYL 2014/212

Teşekkür

We thank Çanakkale Onsekiz Mart University for financial support. The authors also thank Ersin Karabacak for the identification of the plant.

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