Araştırma Makalesi
BibTex RIS Kaynak Göster
Yıl 2020, , 258 - 274, 15.12.2020
https://doi.org/10.38001/ijlsb.756818

Öz

Destekleyen Kurum

HÜBAK(Harran Üniversitesi Bilisel Araştırmalar Projeleri)

Proje Numarası

17079

Kaynakça

  • 1. Yan, H., et al., Purification and characterization of an endo-1, 4-β-glucanase from Bacillus cereus. African Journal of Biotechnology, 2011. 10(72): p. 16277-16285.
  • 2. Sıbtain Ahmed, A.B., Huma Saleem, Mubshara Saadia And Amer Jamil, Production And Purification Of Cellulose Degrading Enzymes From A Filamentous Fungus Trichoderma Harzianum. Pakistan Journal of Botanic, 2009. 41(3): p. 1411-1419.
  • 3. Sukumaran, R.K., R.R. Singhania, and A. Pandey, Microbial cellulases-production, applications and challenges. 2005.
  • 4. Maurya, D.P., et al., Optimization of solid state fermentation conditions for the production of cellulase by Trichoderma reesei. Journal of Environmental Biology, 2012. 33(1): p. 5.
  • 5. Samuels, G.J., Trichoderma: a review of biology and systematics of the genus. Mycological research, 1996. 100(8): p. 923-935.
  • 6. Rifai, M.A., A revision of the genus Trichoderma. Mycological papers, 1969. 116: p. 1-56.
  • 7. AYDIN, M.H., Bitki fungal hastalıklarıyla biyolojik savaşta Trichoderma’lar. Türkiye Tarımsal Araştırmalar Dergisi, 2015. 2(2): p. 135-148.
  • 8. Pandey, S., et al., Trichoderma species cellulases produced by solid state fermentation. J Data Mining Genomics Proteomics, 2015. 6(2): p. 1-4.
  • 9. Shafique, S., R. Bajwa, and S. Shafique, Cellulase biosynthesis by selected Trichoderma species. Pak J Bot, 2009. 41(2): p. 907-916.
  • 10. Zeng, R., et al., A Novel Cellulase Produced by a Newly Isolated Trichoderma virens. Bioengineering (Basel), 2016. 3(2).
  • 11. Kannangara, S., R.M.G.C.S. Dharmarathna, and D.L. Jayarathna, Isolation, Identification and Characterization of Trichoderma Species as a Potential Biocontrol Agent against Ceratocystis paradoxa. Journal of Agricultural Sciences, 2017. 12(1).
  • 12. Ghose, T., Measurement of cellulase activities. Pure and applied Chemistry, 1987. 59(2): p. 257-268.
  • 13. Florencio, C., S. Couri, and C.S. Farinas, Correlation between Agar Plate Screening and Solid-State Fermentation for the Prediction of Cellulase Production by Trichoderma Strains. Enzyme Res, 2012. 2012: p. 793708.
  • 14. Zhang, X.-Y., et al., Development of Trichoderma reesei mutants by combined mutagenesis and induction of cellulase by low-cost corn starch hydrolysate. Process Biochemistry, 2017. 54: p. 96-101.
  • 15. Hassan, M.M., A. Gaber, and E. El-Hallous, Molecular and morphological characterization of Trichoderma harzianum from different Egyptian soils. Wulfenia Journal, 2014. 21: p. 80-96.
  • 16. Shahid, M., et al., Sequencing of 28SrRNA gene for identification of Trichoderma longibrachiatum 28CP/7444 species in soil sample. International Journal of Biotechnology for Wellness Industries, 2013. 2(2): p. 84-90.
  • 17. Kim, K.-C., et al., Isolation and Characteristics of Trichoderma harzianum FJI producing cellulases and xylanase. Journal of microbiology and biotechnology, 2003. 13(1): p. 1-8.
  • 18. Mangalanayaki, R. and S. Madhavan, Cellulase production by Trichoderma harzianum and Fusarium oxysporum under solid state fermentation. WJPPS, 2015. 4: p. 1822-1828.
  • 19. Hafiz Muhammad Nasir, I., et al., Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility. Advances in Bioscience and Biotechnology, 2011. 2011.
  • 20. Pirzadah, T., et al., Characterization of Actinomycetes and Trichoderma spp. for cellulase production utilizing crude substrates by response surface methodology. SpringerPlus, 2014. 3(1): p. 622.
  • 21. Shanmugapriya, K., et al., Isolation, screening and partial purification of cellulase from cellulase producing bacteria. Int J Adv Biotechnol Res, 2012. 3: p. 509-514.
  • 22. Noronha, E.F. and C. Ulhoa, Characterization of a 29-kDa β-1, 3-glucanase from Trichoderma harzianum. FEMS microbiology letters, 2000. 183(1): p. 119-123.
  • 23. Saravanakumar, K. and K. Kathiresan, Bioconversion of lignocellulosic waste to bioethanol by Trichoderma and yeast fermentation. 3 Biotech, 2014. 4(5): p. 493-499.
  • 24. Behera, B.C., et al., Microbial cellulases - Diversity & biotechnology with reference to mangrove environment: A review. J Genet Eng Biotechnol, 2017. 15(1): p. 197-210.
  • 25. Gautam, S., et al., Optimization of the medium for the production of cellulase by the Trichoderma viride using submerged fermentation. International journal of environmental sciences, 2010. 1(4): p. 656-665.
  • 26. Olsson, L., et al., Influence of the carbon source on production of cellulases, hemicellulases and pectinases by Trichoderma reesei Rut C-30. Enzyme and Microbial Technology, 2003. 33(5): p. 612-619.
  • 27. Araujo, R., M. Casal, and A. Cavaco-Paulo, Application of enzymes for textile fibres processing. Biocatalysis and Biotransformation, 2008. 26(5): p. 332-349.
  • 28. Topuz, U., Ö.E. Kıran, and U. Çömlekçioğlu, Selülaz Üreticisi Bacillus Suşlarının Enzimatik Özelliklerinin Araştırılması. 2007.
  • 29. Bischoff, K.M., et al., Purification and characterization of a family 5 endoglucanase from a moderately thermophilic strain of Bacillus licheniformis. Biotechnology Letters, 2006. 28(21): p. 1761-1765.
  • 30. Hurst, P.L., et al., Purification and properties of a cellulase from Aspergillus niger. Biochemical Journal, 1977. 165(1): p. 33-41.
  • 31. Krishna, S.H., et al., Studies on the production and application of cellulase from Trichoderma reesei QM-9414. Bioprocess Engineering, 2000. 22(5): p. 467-470.
  • 32. de Castro, A.M., et al., High-yield endoglucanase production by Trichoderma harzianum IOC-3844 cultivated in pretreated sugarcane mill byproduct. Enzyme research, 2010. 2010.

Production and Biochemical Characterization of Cellulase Enzyme by Trichoderma Strains from Harran Plain

Yıl 2020, , 258 - 274, 15.12.2020
https://doi.org/10.38001/ijlsb.756818

Öz

In this research, Trichoderma spp. fungus, a major producer of cellulase, that was isolated from Harran plain had been investigated. Morphological, microscopic and genetic identification of the six fungi isolates were carried out and their cellulase production ability were determined. The ITS region of four of the isolates were displayed over 90% similarity with the DNA sequences of Trichoderma spp. currently deposited into the databases. The molecular size of fungal cellulase was found as to be 32 kDa by SDS-PAGE analysis. Optimum working conditions of cellulase had also been studied. The optimal temperature for cellulase enzyme activity was 40 ° C, optimum pH 5.0, best reacting time 60 min and ideal substrate concentration was determined as 2% CMC. The fungal cellulase activity was compared with the activity of the commercial cellulase enzyme. The fermentation produced enzyme by using Trichoderma spp. isolated from Harran plain displayed remarkable cellulase activity.

Proje Numarası

17079

Kaynakça

  • 1. Yan, H., et al., Purification and characterization of an endo-1, 4-β-glucanase from Bacillus cereus. African Journal of Biotechnology, 2011. 10(72): p. 16277-16285.
  • 2. Sıbtain Ahmed, A.B., Huma Saleem, Mubshara Saadia And Amer Jamil, Production And Purification Of Cellulose Degrading Enzymes From A Filamentous Fungus Trichoderma Harzianum. Pakistan Journal of Botanic, 2009. 41(3): p. 1411-1419.
  • 3. Sukumaran, R.K., R.R. Singhania, and A. Pandey, Microbial cellulases-production, applications and challenges. 2005.
  • 4. Maurya, D.P., et al., Optimization of solid state fermentation conditions for the production of cellulase by Trichoderma reesei. Journal of Environmental Biology, 2012. 33(1): p. 5.
  • 5. Samuels, G.J., Trichoderma: a review of biology and systematics of the genus. Mycological research, 1996. 100(8): p. 923-935.
  • 6. Rifai, M.A., A revision of the genus Trichoderma. Mycological papers, 1969. 116: p. 1-56.
  • 7. AYDIN, M.H., Bitki fungal hastalıklarıyla biyolojik savaşta Trichoderma’lar. Türkiye Tarımsal Araştırmalar Dergisi, 2015. 2(2): p. 135-148.
  • 8. Pandey, S., et al., Trichoderma species cellulases produced by solid state fermentation. J Data Mining Genomics Proteomics, 2015. 6(2): p. 1-4.
  • 9. Shafique, S., R. Bajwa, and S. Shafique, Cellulase biosynthesis by selected Trichoderma species. Pak J Bot, 2009. 41(2): p. 907-916.
  • 10. Zeng, R., et al., A Novel Cellulase Produced by a Newly Isolated Trichoderma virens. Bioengineering (Basel), 2016. 3(2).
  • 11. Kannangara, S., R.M.G.C.S. Dharmarathna, and D.L. Jayarathna, Isolation, Identification and Characterization of Trichoderma Species as a Potential Biocontrol Agent against Ceratocystis paradoxa. Journal of Agricultural Sciences, 2017. 12(1).
  • 12. Ghose, T., Measurement of cellulase activities. Pure and applied Chemistry, 1987. 59(2): p. 257-268.
  • 13. Florencio, C., S. Couri, and C.S. Farinas, Correlation between Agar Plate Screening and Solid-State Fermentation for the Prediction of Cellulase Production by Trichoderma Strains. Enzyme Res, 2012. 2012: p. 793708.
  • 14. Zhang, X.-Y., et al., Development of Trichoderma reesei mutants by combined mutagenesis and induction of cellulase by low-cost corn starch hydrolysate. Process Biochemistry, 2017. 54: p. 96-101.
  • 15. Hassan, M.M., A. Gaber, and E. El-Hallous, Molecular and morphological characterization of Trichoderma harzianum from different Egyptian soils. Wulfenia Journal, 2014. 21: p. 80-96.
  • 16. Shahid, M., et al., Sequencing of 28SrRNA gene for identification of Trichoderma longibrachiatum 28CP/7444 species in soil sample. International Journal of Biotechnology for Wellness Industries, 2013. 2(2): p. 84-90.
  • 17. Kim, K.-C., et al., Isolation and Characteristics of Trichoderma harzianum FJI producing cellulases and xylanase. Journal of microbiology and biotechnology, 2003. 13(1): p. 1-8.
  • 18. Mangalanayaki, R. and S. Madhavan, Cellulase production by Trichoderma harzianum and Fusarium oxysporum under solid state fermentation. WJPPS, 2015. 4: p. 1822-1828.
  • 19. Hafiz Muhammad Nasir, I., et al., Purification and characterization of the kinetic parameters of cellulase produced from wheat straw by Trichoderma viride under SSF and its detergent compatibility. Advances in Bioscience and Biotechnology, 2011. 2011.
  • 20. Pirzadah, T., et al., Characterization of Actinomycetes and Trichoderma spp. for cellulase production utilizing crude substrates by response surface methodology. SpringerPlus, 2014. 3(1): p. 622.
  • 21. Shanmugapriya, K., et al., Isolation, screening and partial purification of cellulase from cellulase producing bacteria. Int J Adv Biotechnol Res, 2012. 3: p. 509-514.
  • 22. Noronha, E.F. and C. Ulhoa, Characterization of a 29-kDa β-1, 3-glucanase from Trichoderma harzianum. FEMS microbiology letters, 2000. 183(1): p. 119-123.
  • 23. Saravanakumar, K. and K. Kathiresan, Bioconversion of lignocellulosic waste to bioethanol by Trichoderma and yeast fermentation. 3 Biotech, 2014. 4(5): p. 493-499.
  • 24. Behera, B.C., et al., Microbial cellulases - Diversity & biotechnology with reference to mangrove environment: A review. J Genet Eng Biotechnol, 2017. 15(1): p. 197-210.
  • 25. Gautam, S., et al., Optimization of the medium for the production of cellulase by the Trichoderma viride using submerged fermentation. International journal of environmental sciences, 2010. 1(4): p. 656-665.
  • 26. Olsson, L., et al., Influence of the carbon source on production of cellulases, hemicellulases and pectinases by Trichoderma reesei Rut C-30. Enzyme and Microbial Technology, 2003. 33(5): p. 612-619.
  • 27. Araujo, R., M. Casal, and A. Cavaco-Paulo, Application of enzymes for textile fibres processing. Biocatalysis and Biotransformation, 2008. 26(5): p. 332-349.
  • 28. Topuz, U., Ö.E. Kıran, and U. Çömlekçioğlu, Selülaz Üreticisi Bacillus Suşlarının Enzimatik Özelliklerinin Araştırılması. 2007.
  • 29. Bischoff, K.M., et al., Purification and characterization of a family 5 endoglucanase from a moderately thermophilic strain of Bacillus licheniformis. Biotechnology Letters, 2006. 28(21): p. 1761-1765.
  • 30. Hurst, P.L., et al., Purification and properties of a cellulase from Aspergillus niger. Biochemical Journal, 1977. 165(1): p. 33-41.
  • 31. Krishna, S.H., et al., Studies on the production and application of cellulase from Trichoderma reesei QM-9414. Bioprocess Engineering, 2000. 22(5): p. 467-470.
  • 32. de Castro, A.M., et al., High-yield endoglucanase production by Trichoderma harzianum IOC-3844 cultivated in pretreated sugarcane mill byproduct. Enzyme research, 2010. 2010.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Endüstriyel Biyoteknoloji
Bölüm Araştırma Makaleleri
Yazarlar

Mümine Guruk 0000-0002-9004-1994

Mehmet Karaaslan 0000-0001-8097-9535

Proje Numarası 17079
Yayımlanma Tarihi 15 Aralık 2020
Yayımlandığı Sayı Yıl 2020

Kaynak Göster

EndNote Guruk M, Karaaslan M (01 Aralık 2020) Production and Biochemical Characterization of Cellulase Enzyme by Trichoderma Strains from Harran Plain. International Journal of Life Sciences and Biotechnology 3 3 258–274.


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