ANALYSIS OF IL-1Ra AND IL-4 GENE VNTRS POLYMORPHISMS AMONG DENTAL LABORATORY TECHNICIANS: A GENOTYPE-PHENOTYPE STUDY

Yıl 2021, Cilt: 9 Sayı: 3, 831 - 845, 29.11.2021

Burcu Yüksel Serap Yıldırım

https://doi.org/10.33715/inonusaglik.849794

Cited By: 1

Öz

For people working in dental prosthesis laboratories (DPL), metal alloys and methylmethacrylate-based monomers and polymers used in the laboratory can pose occupational risks. These risks can cause occupational health problems and toxic effects on human health. This study purposes of researching the effects of occupational exposure because of dental prosthesis manufacturing operations on variable number tandem repeat (VNTR) polymorphisms in IL-1Ra and IL-4 genes. 29 DPL workers, the experimental group, were given a questionnaire, including the symptoms that might arise due to occupational exposure, and their responses were compared with 30 participants as a control group. Genotype distributions and allele frequencies for IL-1Ra intron 2 (rs2234663) and IL-4 intron 3 (rs79071878) VNTR polymorphisms were analyzed using the PCR technique. Both IL-1Ra intron 2 and IL-4 intron 3 polymorphisms identified from the experimental and control groups were not statistically significantly different (p >.05). However, runny nose, burning throat, dyspnea and dryness and rash on the skin were significantly different when DPL workers were compared with the control group according to logistic regression analysis (p <.05). No statistical difference was found in other phenotypic characters. However, due to the limited sample size, our results need to be confirmed in large-scale studies.

Anahtar Kelimeler

Dental Laboratory Technicians, interleukin-1 receptor antagonist, interleukin-4, Occupational health and safety, polymorphism

Diş Laboratuvarı Teknisyenlerinde IL-1Ra ve IL-4 Geni VNTR Polimorfizmlerinin Analizi: Genotip-Fenotip Çalışması

Öz

Diş protez laboratuvarlarında (DPL) çalışan kişiler için laboratuvarda kullanılan metal alaşımları ve metilmetakrilat bazlı monomerler ve polimerler mesleki risk oluşturabilmektedir. Bu riskler mesleğe bağlı sağlık problemlerine ve insan sağlığında toksik etkilere sebep olabilir. Bu çalışmanın amacı dental protez üretim operasyonlarına bağlı mesleki maruziyetin IL-1Ra ve IL-4 genlerindeki değişken numaralı tandem tekrar (VNTR) polimorfizmleri üzerindeki etkilerini araştırmaktır. 29 DPL çalışanı deney grubu; 30 kontrol grubu ile karşılaştırılarak mesleki maruziyetlerine bağlı ortaya çıkacak semptomları da içine alan anket formları uygulanmıştır. IL-1Ra intron 2 (rs2234663) ve IL-4 intron 3 (rs79071878) VNTR polimorfizmleri için genotip dağılımları ve allel frekansları PCR tekniği kullanılarak analiz edilmiştir. Deney grubu ve kontrol grubundan belirlenen hem IL-1Ra intron 2 hem de IL-4 intron 3 polimorfizmleri istatistiksel olarak anlamlı bulunmamıştır (p >.05). Ancak DPL çalışanları lojistik regresyon analizine göre kontrol grubu ile karşılaştırıldığında burun akıntısı, boğaz yanması, nefes darlığı ve ciltte kuruluk ve kızarıklık gibi alanlarda anlamlı farklılıklar bulundu (p <.05). Bununla birlikte, sınırlı örneklem büyüklüğü nedeniyle, sonuçlarımızın büyük ölçekli çalışmalarda doğrulanması gerekmektedir.

Anahtar Kelimeler

Diş Laboratuvarı Teknisyenleri, interlökin-1 reseptör antagonisti, interlökin-4, iş sağlığı, polimorfizm

Kaynakça

• Abakay, A., Atilgan, S., Abakay, O., Atalay, Y., Guven, S., Yaman, F., Tanrikulu, A. C. (2013). Frequency of respiratory function disorders among dental laboratory technicians working under conditions of high dust concentration. Eur Rev Med Pharmacol Sci, 17(6), 809-814.
• Akkurt, I. (2014). Occupational respiratory diseases. Ankara: Günes Medical Publisher (in Turkish).
• Anlar, H.G., Bacanli, M., İritaş, S., Bal, C., Kurt, T., Tutkun, E., …Basaran, N. (2017) Effects of occupational silica exposure on oxidative stress and immune system parameters in ceramic workers in TURKEY. Journal of Toxicology and Environmental Health, Part A, 80, 13-15.
• Ansteinsson, V. E., Samuelsen, J. T., Dahl, J. E. (2009). Filler particles used in dental biomaterials induce the production and release of inflammatory mediators in vitro. Journal of Biomedical Materials Research Part B: Applied Biomaterials: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials, 89(1), 86-92.
• Arsal Yıldırım S.,Pekey, B. (2018). Assessment of particulate matter-related pollution in the dental prosthesis laboratory air in terms of workers' health. Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 22(2), 838-852.
• Arsal Yıldırım , S. A., Pekey, B., Pekey, H. (2020). Assessment of occupational exposure to fine particulate matter in dental prosthesis laboratories in Kocaeli, Turkey. Environmental monitoring and assessment, 192(10), 1-16.
• Bakopoulou, A., Papadopoulos, T., Garefis, P. (2009). Molecular toxicology of substances released from resin-based dental restorative materials. International journal of molecular sciences, 10(9), 3861-3899.
• Bernstein, J. A., Alexis, N., Bacchus, H., Bernstein, I. L., Fritz, P., Horner, E., Reijula, K. (2008). The health effects of nonindustrial indoor air pollution. Journal of Allergy and Clinical Immunology, 121(3), 585-591.
• Bingöl, G., Polat, F., Diler, S. B. (2020). Association of IL-1RA and IL-4 gene vntrs with susceptibility to prostate cancer in the Turkish population. Cytology and Genetics, 54(5), 487-492.
• Birbian, N., Singh, J., Jindal, S. K., Sobti, R. C. (2014). High risk association of IL-4 VNTR polymorphism with asthma in a North Indian population. Cytokine, 66(1), 87-94.
• Burgaz, S., Demircigil, G. Ç., Yılmazer, M., Ertaş, N., Kemaloǧlu, Y., Burgaz, Y. (2002). Assessment of cytogenetic damage in lymphocytes and in exfoliated nasal cells of dental laboratory technicians exposed to chromium, cobalt, and nickel. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 521(1-2), 47-56.
• Büyüköztürk, S., Gelincik, A., Ünal, D., Demirtürk, M., Çelik, D.D., Erden, S., …Erdem Kuruca, S.(2015). Oral nickel exposure may induce Type I hypersensitivity reaction in nickel-sensitized subjects. Int Immunopharmacol., 26(1), 92-96.
• Choël, L., Grosgogeat, B., Bourgeois, D., Descotes, J. (1999). Occupational toxic risks in dental laboratory technicians. Journal of Environmental Medicine, 1(4), 307-314.
• Chuang, H. Y., Yu, K. T., Ho, C. K., Wu, M. T., Lin, G. T., Wu, T. N. (2004). Investigations of vitamin D receptor polymorphism affecting workers’ susceptibility to lead. Journal of occupational health, 46(4), 316-322.
• Cokic, S. M., Hoet, P., Godderis, L., Wiemann, M., Asbach, C., Reichl, F. X., Van Landuyt, K. L. (2016). Cytotoxic effects of composite dust on human bronchial epithelial cells. Dental Materials, 32(12), 1482-1491.
• Dejan, T., Mirkovska, S. J., Aleksandar, P., Ana, S., Efinska, M. O., Emilija, S., Jean, G. (2009). Association of Cytokine Gene Polymorphisms with Chronic Obstructive. Iranian Journal of Allergy, Asthma and Immunology, 31-42.
• Durner, J., Wellner, P., Hickel, R., Reichl, F. X. (2012). Synergistic interaction caused to human gingival fibroblasts from dental monomers. Dental Materials, 28(8), 818-823.
• Echeverria, D., Woods, J. S., Heyer, N. J., Rohlman, D., Farin, F. M., Li, T., Garabedian, C. E. (2006). The association between a genetic polymorphism of coproporphyrinogen oxidase, dental mercury exposure and neurobehavioral response in humans. Neurotoxicology and teratology, 28(1), 39-48.
• Fang, Z., Zhao, M., Zhen, H., Chen, L., Shi, P., Huang, Z. (2014). Genotoxicity of tri-and hexavalent chromium compounds in vivo and their modes of action on DNA damage in vitro. PloS one, 9(8), e103194
• Garcia-Fernandez, A. J., Bayoumi, A. E., Perez-Pertejo, Y., Motas, M., Reguera, R. M., Ordonez, C., Ordonez, D. (2002). Alterations of the glutathione–redox balance induced by metals in CHO-K1 cells. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 132(3), 365-373
• Gupta, S., Rani, S., Garg, S. (2017). Infection control knowledge and practice: A cross-sectional survey on dental laboratories in dental institutes of North India. The Journal of the Indian Prosthodontic Society, 17(4), 348.
• Hariyani, N., Berniyanti, T., Setyowati, D. (2015). Effects of occupational, environmental controls on the level of Co, Ni and Cr among dental technicians. International Journal of Environmental Science and Development, 6(9), 643-647.
• Hirano, T., Numakura, T., Moriyama, H., Saito, R., Shishikura, Y., Shiihara, J., Ichinose, M. (2018). The first case of multiple pulmonary granulomas with amyloid deposition in a dental technician; a rare manifestation as occupational lung disease. BMC pulmonary medicine, 18(1), 77.
• Hu, S. W., Lin, Y. Y., Wu, T. C., Hong, C. C., Chan, C. C., Lung, S. C. C. (2006). Workplace air quality and lung function among dental laboratory technicians. American journal of industrial medicine, 49(2), 85-92.
• Huang, K.L., Lee, Y.H., Chen, H.I., Liao, H.S., Chiang, B.L., Cheng, T.J. (2015). Zinc oxide nanoparticles induce eosinophilic airway inflammation in mice. J Hazard Mater., 297,304-12.
• Jongh, C.M., Verberk, M.M., Withagen, C.E., Jacobs, J.J., Rustemeyer, T., Kezic, S (2006) Stratum corneum cytokines and skin irritation response to sodium lauryl sulfate. Contact Dermatitis 54, 325–33.
• Joos, L., McIntyre, L., Ruan, J., Connett, J. E., Anthonisen, N. R., Weir, T. D., Sandford, A. J. (2001). Association of IL-1β and IL-1 receptor antagonist haplotypes with the rate of decline in lung function in smokers. Thorax, 56(11), 863-866.
• Kesarwani, P., Ahirwar, D. K., Mandhani, A., Mittal, R. D. (2008). Association between− 174 G/C promoter polymorphism of the interleukin-6 gene and progression of prostate cancer in North Indian population. DNA and cell biology, 27(9), 505-510.
• Kettelarij, J., Nilsson, S., Midander, K., Lidén, C., Julander, A. (2016). Snapshot of cobalt, chromium and nickel exposure in dental technicians. Contact Dermatitis, 75(6), 370-376.
• Kezic, S., Visser, M.J., Verberk, M.M. (2009). Individual susceptibility to occupational contact dermatitis. Industrial Health, 47, 469–478.
• Kim, T. S., Kim, H. A., Heo, Y., Park, Y., Park, C. Y., Roh, Y. M. (2002). Level of silica in the respirable dust inhaled by dental technicians with demonstration of respirable symptoms. Industrial Health, 40(3), 260-265.
• Kuran, G., Aslan, H., Haytoğlu, S., Yüreğir, Ö. Ö., Bozdoğan, S. T. (2019). IL-1RN VNTR, IL-2 (-330), and IL-4 VNTR gene polymorphisms in patients with chronic rhinosinusitis with sinonasal polyposis. Turkish Journal of medical sciences, 49(5), 1411-1417.
• Mathisen, G. H., Ansteinsson, V., Samuelsen, J. T., Becher, R., Dahl, J. E., Bølling, A. K. (2015). TEGDMA and filler particles from dental composites additively attenuate LPS-induced cytokine release from the macrophage cell line RAW 264.7. Clinical oral investigations, 19(1), 61-69.
• Movahedi, M., Mahdaviani, S. A., Rezaei, N., Moradi, B., Dorkhosh, S., Amirzargar, A. A. (2008). IL-10, TGF-ß, IL-2, IL-12, and IFN-γ Cytokine Gene Polymorphisms in Asthma. Journal of Asthma, 45(9), 790-794.
• Milonski, J., Zielinska-Blizniewska, H., Majsterek, I., Przybyłowska-Sygut, K., Sitarek, P., Korzycka-Zaborowska, B., Olszewski, J. (2015). Expression of POSTN, IL-4, and IL-13 in chronic rhinosinusitis with nasal polyps. DNA and Cell Biology, 34(5), 342-349.
• Mout, R., Willemze, R., Landegent, J. E. (1991). Repeat polymorphisms in the interleukin-4 gene (IL4). Nucleic Acids Research, 19(13), 3763.
• Nayebzadeh, A., Dufresne, A. (1999). Evaluation of exposure to methyl methacrylate among dental laboratory technicians. American Industrial Hygiene Association Journal, 60(5), 625-628.
• Okamoto, M., Tominaga, M., Shimizu, S., Yano, C., Masuda, K., Nakamura, M., Kawayama, T. (2017). A Case of Dental Technicians' Pneumoconiosis. Internal Medicine, 8860-17.
• Otsuki, T., Maeda, M., Murakami, S., Hayashi, H., Miura, Y., Kusaka, M., …Nishimura, Y. (2007). Immunological effects of silica and asbestos. Cell Mol Immunol., (4)261-268.
• Palabıyık, S.S.,Girgin, G.,Tutkun, E., Hiçyılmaz, O.H.,Baydar, T. (2013). Immunomodulation and oxidative stress in denim sandblasting workers: changes caused by silica exposure. Arh Hig Rada Toksikol., 64, 431-437.
• Pukanha, K., Yimithrang, S., Kwanhian, W. (2020). The immunotoxicity of chronic exposure to high levels of lead: an ex vivo investigation. Toxics, 8, (56), 1-13.
• Rabbani-Chadegani, A. (2013). Studies on the genotoxic effect of chromium oxide (Cr VI): Interaction with deoxyribonucleic acid in solution. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 750(1-2), 105-110.
• Salimi, S., Mohammadoo-Khorasani, M., Yaghmaei, M., Mokhtari, M., Moossavi, M. (2014). Possible association of IL-4 VNTR polymorphism with susceptibility to preeclampsia. BioMed research international, 2014.
• Settin, A., Zedan, M., Farag, M., El Regal, M. E., Osman, E. (2008). Gene polymorphisms of IL-6− 174 G/C and IL-1Ra VNTR in asthmatic children. The Indian Journal of Pediatrics, 75(10), 1019-1023.
• Sinitchi, G. (2017). Zirconium allergies caused by oral dental materials. A general review. International Journal of Medical Dentistry, 21(2).
• Sosroseno, W., Herminajeng, E., Goeno, S. (1994). The interleukin network in the immunopathogenesis of oral diseases. Asian Pacific Journal of allergy and immunology, 12(2), 161.
• Tadin, A., Gavic, L., Jurkovic, I., Vidovic, N., Jerkovic, D., Zeljezic, D. (2019). Cytogenetic Biomonitoring of Dental Technicians: A Cross‐Sectional Study. Journal of Prosthodontics, 28(2), 106-112.
• Tillie-Leblond, I., Pugin, J., Marquette, C. H., Lamblin, C., Saulnier, F., Brichet, A., Gosset, P. (1999). Balance between pro-inflammatory cytokines and their inhibitors in bronchial lavage from patients with status asthmaticus. American journal of respiratory and critical care medicine, 159(2), 487-494.
• Tsai, F. J., Chang, C. H., Chen, C. C., Hsia, T. C., Chen, H. Y., Chen, W. C. (2005). Interleukin4 gene intron 3 polymorphism is associated with transitional cell carcinoma of the urinary bladder. BJU international, 95(3), 432-435.
• Wan, J. X., Zhang, Z. B., Guan, J. R., Cao, D. Z., Ye, R., Jin, X. P., Xıa, Z. L. (2006). Genetic Polymorphism of Toxicant Metabolizing Enzymes and Prognosis of Chinese Workers with Chronic Benzene Poisoning. Annals of the New York Academy of Sciences, 1076(1), 129-136.
• Yagi, R., Tanaka, S., Motomura, Y., Kubo, M. (2007). Regulation of the Il4 gene is independently controlled by proximal and distal 3′ enhancers in mast cells and basophils. Molecular and cellular biology, 27(23), 8087-8097.
• Yuksel, B. (2021). Investigation of morphological abnormalities in red blood cells among dental laboratory technicians. Environmental Science and Pollution Research, 28(16), 20650-20658.
• Yuksel, B., Arsal Yildirim, S. (2021). Determination of cytogenetic abnormalities in buccal mucosa of dental laboratory technicians. Archives of Environmental & Occupational Health, 1-8.
• Zamani Badi, T., Nikzad, H., Karimian, M. (2018). IL‐1RA VNTR and IL‐1α 4845G> T polymorphisms and risk of idiopathic male infertility in Iranian men: A case-control study and an in silico analysis. Andrologia, 50(9), e13081.
• Zhang, M. L., Ni, P. H., Cai, C. P., Chen, N. J., Wang, S. L. (2012). Association of susceptibility to chronic rhinosinusitis with genetic polymorphisms of IL-4 and IL-10. Zhonghua er bi yan hou tou jing wai ke za zhi= Chinese journal of otorhinolaryngology head and neck surgery, 47(3), 212-217.