L-Sistein ve SigB'nin Listeria monocytogenes 10403S'nin termal toleransı üzerindeki oksijene bağlı etkileri

Öz

Listeria monocytogenes, ısı, oksidatif ve ozmotik stresler dahil olmak üzere çok çeşitli çevresel koşullara dayanabilen önemli bir gıda kaynaklı patojendir. Alternatif sigma faktörü SigB, bakterinin stres adaptasyonunda merkezi bir rol oynamaktadır. Ancak oksijenin sınırlı olduğu koşullar ve besin açısından zengin ortamlardaki işlevi henüz tam olarak aydınlatılamamıştır. Et ve et ürünleri, süt ürünleri ve baklagillerinde yaygın olarak bulunan kükürt içeren bir amino asit olan L-sistein, hem metabolik bir sinyal molekülü hem de hücresel antioksidan sistemlerin öncüsü olarak stres toleransını etkileyebileceği önceki çalışmalarda gösterilmiştir. Bu çalışmada, hücre dışı L-sistein takviyesinin L. monocytogenes'in ısı direnci üzerindeki etkisi incelenmiş ve aerobik ile anaerobik büyüme koşulları altında SigB'nin rolü değerlendirilmiştir. Yabanıl tip L. monocytogenes 10403S suşu ile izojenik ΔsigB mutantı, 1,57 mM L-sistein ile takviye edilmiş tanımlı besiyerinde, kontrollü aerobik ve anaerobik koşullar altında 56°C'de ısı stresine maruz bırakılmıştır. Bakteriyel hayatta kalma oranı kantitatif olarak belirlenmiş ve elde edilen veriler farklı suşlar ve çevresel koşullar arasında karşılaştırmalı olarak analiz edilmiştir. Elde edilen bulgular, L-sistein takviyesinin özellikle anaerobik koşullarda uygulanan ısı stresi altında bakteriyel hayatta kalmayı anlamlı düzeyde artırdığını göstermiştir. Ayrıca, ΔsigB mutantının bu koşullarda yabanıl tip suşa kıyasla ısıl strese karşı daha yüksek direnç sergilediği belirlenmiştir. Bu sonuçlar, L-sistein metabolizması veya ilişkili biyokimyasal yolakların, SigB aracılı stres yanıtının yokluğunu kısmen telafi edebileceğini düşündürmektedir. Sonuç olarak, L-sistein mevcudiyeti, oksijen düzeyi ve SigB faktörü, L. monocytogenes'in ısı stresine karşı hayatta kalmasını bağlama bağlı olarak birlikte şekillendirmektedir. Bu etkileşimlerin daha iyi anlaşılması, gıda ürünleri ve işleme ortamlarında L. monocytogenes'in kontrolüne yönelik daha etkili ve hedefe yönelik stratejilerin geliştirilmesine katkı sağlayabilir.

Anahtar Kelimeler

• Listeria monocytogenes
• Isı stresi direnci
• SigB (σ^B)
• L-sistein
• Anaerobik koşullar

Oxygen-dependent effects of L-Cysteine and SigB on thermal tolerance of Listeria monocytogenes 10403S

Öz

Listeria monocytogenes is a resilient foodborne pathogen capable of surviving diverse environmental stresses, including heat, oxidative, and osmotic conditions. The alternative sigma factor SigB plays a central role in mediating stress adaptation. However, its function under oxygen-limited conditions and in nutrient-rich environments remains insufficiently understood. L-cysteine, commonly present in food matrices, may influence bacterial stress tolerance by acting as both a metabolic signal and a precursor for antioxidant systems. This study investigated the effect of extracellular L-cysteine supplementation on the heat resistance of L. monocytogenes and evaluated the contribution of SigB under both aerobic and anaerobic conditions. Wild-type L. monocytogenes 10403S and an isogenic ΔsigB mutant were subjected to heat stress in defined medium supplemented with 1.57 mM L-cysteine. Bacterial survival was quantified and compared across strains and environmental conditions. L-cysteine supplementation significantly enhanced bacterial survival under anaerobic heat stress. Notably, the ΔsigB mutant exhibited greater resistance than the wild-type strain under these conditions. This observation suggests that L-cysteine-associated metabolic pathways may compensate, at least partially, for the absence of SigB-mediated stress regulation. The enhanced survival in the mutant strain points to alternative protective mechanisms, potentially linked to redox balance or sulphur metabolism. Overall, the findings demonstrate that L-cysteine availability, oxygen conditions, and SigB interact in a complex and context-dependent manner to influence heat stress survival in L. monocytogenes. These results highlight the importance of metabolic state in shaping bacterial stress responses and suggest that sulphur metabolism may serve as a key compensatory pathway under oxygen-limited conditions. A deeper understanding of these interactions could support the development of more effective strategies for controlling L. monocytogenes in food systems and processing environments.

Anahtar Kelimeler

• Listeria monocytogenes
• Heat stress resistance
• SigB (σ^B)
• L-cysteine
• Anaerobic conditions

Kaynakça

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