Making Butter and Milk from Plants: An Investigation into Plant-Based Butter as a Novel Approach

Öz

Plant-based milk and dairy analogues are gaining increasing attention as sustainable alternatives to animal-derived products, which are often associated with environmental and health concerns. This study aimed to develop and characterize a novel plant-based butter produced from flaxseed milk. Flaxseed was processed to obtain plant-based milk, which was subsequently used to formulate a plant-based butter. The product was evaluated in terms of sensory acceptance, pH, titratable acidity, dry matter, color parameters, and fatty acid composition. Sensory analysis indicated that flaxseed-based butter exhibited acceptable consumer attributes. Fatty acid profiling revealed nine compounds, with lauric acid (38.42%), oleic acid (18.36%), and myristic acid (15.16%) identified as the predominant fatty acids. The findings demonstrate that flaxseed-based butter has promising potential as a sustainable alternative in the category of plant-based dairy products, owing to its favorable sensory properties and suitable fatty acid profile.

Anahtar Kelimeler

• Butter quality
• fatty acid profile
• flaxseed milk
• plant-based food
• sustainability
• vegan diet

Bitki-bazlı süt ve tereyağı üretimi: Bitki-bazlı tereyağının yenilikçi bir yaklaşım olarak incelenmesi

Öz

Plant-based milk and dairy analogues are gaining increasing attention as sustainable alternatives to animal-derived products, which are often associated with environmental and health concerns. This study aimed to develop and characterize a novel plant-based butter produced from flaxseed milk. Flaxseed was processed to obtain plant-based milk, which was subsequently used to formulate a plant-based butter. The product was evaluated in terms of sensory acceptance, pH, titratable acidity, dry matter, color parameters, and fatty acid composition. Sensory analysis indicated that flaxseed-based butter exhibited acceptable consumer attributes. Fatty acid profiling revealed nine compounds, with lauric acid (38.42%), oleic acid (18.36%), and myristic acid (15.16%) identified as the predominant fatty acids. The findings demonstrate that flaxseed-based butter has promising potential as a sustainable alternative in the category of plant-based dairy products, owing to its favorable sensory properties and suitable fatty acid profile.

Anahtar Kelimeler

• Butter quality
• fatty acid profile
• flaxseed milk
• plant-based food
• sustainability
• vegan diet

Kaynakça

1. Ahmed, A., Sayed, M., Kamal, S., Abdel-Hafeez, O., 2022. Physicochemical evaluation of cooking butter and hydrogenated oils. Assiut Veterinary Medical Journal, 68 (175), 106–114. https://doi.org/10.21608/avmj.2022.157084.1080
2. Akgül, H. İ., 2015. Trabzon tereyağlarının bazı kalite parametrelerinin belirlenmesi. Atatürk Üniversitesi Fen Bilimleri Enstitüsü, Yüksek lisans tezi. (Basılmış)
3. Altınay, C., Şanlı, T., 2023. Vegan beslenmede alternatif süt ürünleri: Teknolojik ve beslenme yönü. GIDA, 48(6), 1318–1334. https://doi.org/10.15237/gida.GD23114
4. AOAC, 2000. Fatty acids in oils and fats: Official Method 969.33 (17th ed.), Washington, DC.
5. AOAC, 1992. Official Methods of Analysis, Association of Official Analytical Chemists, Washington, DC.
6. Atamer, M., Kaptan, N., 1982. Ankara’da tüketime sunulan kahvaltılık tereyağların nitelikleri üzerine araştırmalar. Gıda, 4, 189–198.
7. Ayar, A., Özcan, M., Sert, D., Arslan, D., 2006. Yayık tereyağının raf ömrünün uzatılmasına bazı baharat, uçucu yağ ve ekstraktlarının katkısı (TÜBİTAK Proje No: TOVAG-105 O 046). Konya.
8. Aydemir, M., 2024. Evaluation of butter produced from whey and milk fat in terms of some quality criteria and fatty acid compositions. Revista Científica De La Facultad De Ciencias Veterinarias, 34(1), 1–6. https://doi.org/10.52973/rcfcv-e34293

9. Aydın, S., Kahyaoğlu, D., 2020. Antioxidant effect potential of garlic in vitro and real food system: Effects of garlic supplementation on oxidation stability and sensory properties of butter. European Journal of Lipid Science and Technology, 122(3). https://doi.org/10.1002/ejlt.201900261
10. Becskei, Z., Savić, M., Ćirković, D., Rašeta, M., Puvača, N., Pajić, M. and Paskaš, S., 2020. Assessment of water buffalo milk and traditional milk products in a sustainable production system. Sustainability, 12(16), 6616. https://doi.org/10.3390/su12166616
11. Bhatty, R.S., Cherdkiatgumchai, P., 1990. Compositional analysis of laboratory-prepared and commercial samples of linseed meal and of hulls. Journal of the American Oil Chemists’ Society, 67(2), pp. 79–84. https://doi.org/10.1007/BF02540548
12. Bilici, R., 2022. Vegan mutfağı kapsamında geliştirilmiş yenilikçi bir ürün: Bitkisel bazlı pastacı kreması. İstanbul Gelişim Üniversitesi Lisansüstü Eğitim Enstitüsü, Gastronomi, Yüksek lisans tezi.
13. Bloedon, L.T., Szapary, P.O., 2004. Flaxseed and cardiovascular risk. Nutrition reviews, 62(1), pp. 18–27. https://doi.org/10.1111/j.1753-4887.2004.tb00002.x
14. Chen, S., Bobe, G., Zimmerman, S., Hammond, E.G., Luhman, C.M., Boylston, T.D., Freeman, A.E. and Beitz, D.C. 2004. Physical and sensory properties of dairy products from cows with various milk fatty acid compositions. Journal of Agricultural and Food Chemistry, 52(10), pp. 3422–3428.
15. Chen, X., Niu H., McClements, D.J., 2024. Flaxseed oil droplets coated with potato, soy, or pea protein: Formation and stability of plant-based milk fat globules. Food Research International, 197(Part 1), 115175.
16. Costa, A., Pereira, D., Veríssimo, C., Bomfim, M., Queiroga, R., Madruga, M. and Soares, J., 2019. Developing cookies formulated with goat cream enriched with conjugated linoleic acid. PLOS ONE, 14(9), e0212534. https://doi.org/10.1371/journal.pone.0212534
17. Costa, M.A., Kuhn, D., Rama, G.R., Lehn, D.N. and Souza, C.F.V., 2022. Whey butter: A promising perspective for the dairy industry. Brazilian Journal of Food Technology, 25, e2021088. https://doi.org/10.1590/1981-6723.08821
18. Craig, W.J., 2023. Plant based dairy alternatives contribute to a healthy and nutritious diet. Nutrients, 15(15), 33. https://doi.org/10.3390/nu15153393
19. Çakmakçı, S., Kahyaoğlu, D., 2018. Farklı hayvan sütlerinden üretilen tereyağlarının depolama süresince oksidasyon stabilitesi ve bazı özellikleri üzerine karşılaştırmalı bir araştırma. Gıda / The Journal of Food, 43(2), pp. 283–293.
20. Çakmakçı, S., Çelik, K., Ertem, H., Çalışkan, H., 2020. Tereyağı olarak satılan yağlarda bazı hileler ve kalite özelliklerinin tespiti: Erzurum örneği. Gıda, 45, pp. 800–813.
21. Celik, S., Bakirci, I., 2000. A Study on the Physical and Chemical Properties of Cookery-Type Butter. Pakistan Journal of Biological Sciences, 3, 596–598.
22. Daryani, D., Pegua, K., Aryaa, S.S., 2024. Review of plant-based milk analogue: Its preparation, nutritional, physicochemical, and organoleptic properties. Food Science and Biotechnology, 33, 1059–1073. https://doi.org/10.1007/s10068-023-01482-z
23. Escobar-Sáez, D., Montero-Jiménez, L., García Herrera, P. and Sánchez-Mata, M.C., 2022. Plant based drinks for vegetarian or vegan toddlers: Nutritional evaluation of commercial products, and review of health benefits and potential concerns. Food Research International, 160, 111646. https://doi.org/10.1016/j.foodres.2022.111646
24. Eser, İ.H., İnanç, A.L., 2022. Farklı yöntemlerle Anadolu manda tereyağı üretimi. Tekirdağ Ziraat Fakültesi Dergisi, 19(1), pp. 215–226.
25. EC, 1994. European Council Regulation (EC) No 2991/94 of 5 December 1994 laying down standards for spreadable fats.
26. Fatemizadeh, S., Yavarmanesh, M. and Najafi, M., 2017. Survival and partitioning of male‐specific coliphage (MS2) as a surrogate for enteric viruses in the production process of traditional butter. Journal of Food Safety, 37(4), e12344. https://doi.org/10.1111/jfs.12344
27. Fernandes, C.G., Sonawane, S.K., Arya, S.S., 2018. Cereal based functional beverages: A review. Journal of Microbiology, Biotechnology and Food Sciences, 8(3), 914–919. https://doi.org/10.15414/jmbfs.2018-19.8.3.914-919
28. Fındık, O., 2011. Van’da piyasaya sunulan bazı tereyağları ile bu tereyağlardan elde edilen sadeyağların bazı kimyasal ve mikrobiyolojik özelliklerinin karşılaştırılması. Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü, Yüksek lisans tezi.
29. Gobbi, L., Ciano, S., Rapa, M.R., Ruggieri, R. 2019. Biogenic amines determination in “plant milks”. Beverages, 5(2), 40. https://doi.org/10.3390/beverages5020040
30. Idoui, T., Benhamada, N., Leghouchi, E. 2010. Microbial quality, physicochemical characteristics and fatty acid composition of a traditional butter produced from cows’ milk in East Algeria. Grasas y Aceites, 61(3), 232–236. https://grasasyaceites.revistas.csic.es/index.php/grasasyaceites/article/view/994
31. Idoui, T., Rechak, H., Zabayou, N., 2013. Microbial quality, physicochemical characteristics and fatty acid composition of a traditional butter made from goat milk. Annals. Food Science and Technology, 14(1), 108.
32. İşleroğlu, H., Yıldırım, Z. and Yıldırım, M., 2005. Fonksiyonel bir gıda olarak keten tohumu. Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi, 22(2), 23–30.
33. Johnson, A.J., Stevenson, J., Pettit, J., Jasthi, B., Byhre, T. and Harnack, L., 2025. Assessing the Nutrient Content of Plant-Based Milk Alternative Products Available in the United States. Journal of the Academy of Nutrition and Dietetics, 125(4), 515–527.e8. https://doi.org/10.1016/j.jand.2024.06.003
34. Kahyaoğlu, D., Musaoğlu, G., 2022. Kastamonu ilinin merkez köylerinde üretilen tereyağlarının bazı mikrobiyolojik ve fizikokimyasal kalite özelliklerinin belirlenmesi. Mustafa Kemal Üniversitesi Tarım Bilimleri Dergisi, 27(2), 224–233. https://doi.org/10.37908/mkutbd.1036674
35. Kajla, P., Sharma, A., Sood, D.R., 2015. Flaxseed—A potential functional food source. Journal of Food Science and Technology, 52(4), 1857–1871. https://doi.org/10.1007/s13197-014-1290-7
36. Kanyama, C.A., Hedin, B., Katzeff, C., 2021. Differences in Environmental Impact between Plant-Based Alternatives to Dairy and Dairy Products: A Systematic Literature Review. Sustainability, 13(22), 12599. https://doi.org/10.3390/su132212599
37. Lapčíková, B., Lapcik,L., Valenta, T., Chvatikova, M., 2024. Plant based emulsions as dairy cream alternatives: Effect of mono and diglycerides on physico-chemical properties. Foods, 13(8), 1225.
38. Marpalle, P., Sonawane, S.K., Arya, S.S., 2014. Effect of incorporation of flaxseed flour on the physicochemical and sensory properties of functional bread. LWT - Food Science and Technology, 58(2), 614–619.
39. Mehdizadeh, T., Mohammadipour, N., Langroodi, A., Raeisi, M. 2019. Effect of walnut kernel septum membranes hydroalcoholic extract on the shelf life of traditional butter. Heliyon, 5(3), e01296. https://doi.org/10.1016/j.heliyon.2019.e01296
40. Moshtaghian, H., Hallström, E., Bianchi, M., Bryngelsson, S., 2024. Nutritional profile of plant-based dairy alternatives in the Swedish market. Current Research in Food Science, 8, 100712. https://doi.org/10.1016/j.crfs.2024.100712
41. Munekata, P.E.S., Domínguez, R., Budaraju, S., Roselló-Soto, E., Barba, F.J., Mallikarjunan, K., Roohinejad, S. and Lorenzo, J.M., 2020. Effect of innovative food processing technologies on the physicochemical and nutritional properties and quality of non-dairy plant-based beverages. Foods, 9(3), 288. https://doi.org/10.3390/foods9030288
42. Ningtyas, D.W., 2023. Plant-based butter like spreads. In: S. Prakash, B.R. Bhandari and C. Gaiani, eds. Engineering plant-based food systems. Academic Press, 151–166. https://doi.org/10.1016/B978-0-323-89842-3.00013-0
43. Olsmats, E., Rennie, A.R., 2025. From dairy to plant products: Understanding their structural fingerprints with X-rays. NPJ Science of Food, 9, 109. https://doi.org/10.1038/s41538-025-00493-w
44. Oomah, B.D., Mazza, G., 1993. Flaxseed proteins—A review. Food Chemistry, 48(2),109–114. https://doi.org/10.1016/0308-8146(93)90206-6
45. Oracz, Green Kai by Aga, 2025. Contact. Green Kai. Available at: https://www.greenkai.co.nz/contact (Accessed: 4 August 2025)
46. Özcan, M.M., Değerli, Z., 2019. Effect on human health and bioactive components of linseed. Food Industry: Science and Technologies, 12(3)(45), 85–92.
47. Paul, A.A., Kumar, S., Kumar, V., Sharma, R., 2019. Milk analog: Plant-based alternatives to conventional milk, production, potential and health concerns. Critical Reviews in Food Science and Nutrition,1–19. https://doi.org/10.1080/10408398.2019.1674243
48. Plamada, D., Teleky, B.-E., Nemes, S.A., Mitrea, L., Szabo, K., Călinoiu, L.-F., Pascuta, M.S., Varvara, R.-A., Ciont, C., Martău, G.A., Simon, E., Barta, G., Dulf, F.V., Vodnar, D.C. and Nitescu, M. 2023. Plant-based dairy alternatives—A future direction to the milky way. Foods, 12(9), 1883. https://doi.org/10.3390/foods12091883
49. Quezada, C., Urra M., Mella, C., Zuniga, R.N., Troncoso, E., 2024. Plant based oil-in-water food emulsions: Exploring the influence of different formulations on their physicochemical properties. Foods, 13(4), 513.
50. Radtke, T., Schmid, A., Trepp, A., Dähler, F., Coslovsky, M., Eser, P. and Saner, H., 2016. Short-term effects of trans fatty acids from ruminant and industrial sources on surrogate markers of cardiovascular risk in healthy men and women: A randomized, controlled, double-blind trial. European Journal of Preventive Cardiology, 24(5), 534–543. https://doi.org/10.1177/2047487316680691
51. Ramsing R, Santo R, Kim BF, Altema-Johnson D, Wooden A, Chang KB, Semba RD, Love DC. , 2023. Dairy and plant-based milks: Implications for nutrition, health, and environmental impacts. Current Environmental Health Reports, 10(3), 291–302. https://doi.org/10.1007/s40572-023-00400-z
52. Reyes-Jurado, F., Soto-Reyes, N., Dávila-Rodríguez, M., Lorenzo-Leal, A.C., Jiménez-Munguía, M.T., Mani-López, E. and López-Malo, A., 2023. Plant-based milk alternatives: Types, processes, benefits, and characteristics. Food Reviews International, 39(4),2320–2351. https://doi.org/10.1080/87559129.2021.1952421
53. Sagdic, O., Donmez, M., Demirci, M., 2004. Comparison of characteristics and fatty acid profiles of traditional Turkish yayik butters produced from goats', ewes' or cows' milk. Food Control, 15(6), 485–490.
54. Sethi, S., Tyagi, S.K., Anurag, R.K., 2016. Plant-based milk alternatives an emerging segment of functional beverages: A review. Journal of Food Science and Technology, 53(9),3408–3423. https://doi.org/10.1007/s13197-016-2328-3
55. Sevmiş, E., Andiç, S., Oğuz, Ş. 2020. Comparison of the compositions of butter and butter oil produced in the province of Hakkari. YYÜ Tarım Bilimleri Dergisi, 30, 928–937.
56. Siddique, F., Khan, K., Ahmad, A., Ahmed, A., Gülfraz, M. and Wattoo, F., 2011. Development and optimization of sweet cream butter from buffaloes at cottage scale. African Journal of Biotechnology, 10(75). https://doi.org/10.5897/ajb11.2049
57. Siddiqui, S.A., Mehany, T., Schulte, H., Pandiselvam, R., Nagdalian, A.A., Golik, A.B., Shah, M.A., Shahbaz, H.M. and Maqsood, S., 2024. Plant-Based Milk – Thoughts of researchers and industries on what should be called as “milk”. Food Reviews International, 40(6),1703–1730. https://doi.org/10.1080/87559129.2023.2228002
58. Silva, A.R.A., Silva, M.M.N., Ribeiro, B.D., 2020. Review: Health issues and technological aspects of plant-based alternative milk. Food Research International, 131, 108972. https://doi.org/10.1016/j.foodres.2019.108972
59. Silva, T., Pires, A., Gomes, D., Viegas, J., Pereira-Dias, S., Pintado, M. and Pereira, C., 2023. Sheep’s butter and correspondent buttermilk produced with sweet cream and cream fermented by aromatic starter, kefir and probiotic culture. Foods, 12(2), 331. https://doi.org/10.3390/foods12020331
60. Singh, S., Kumar, R. and Rao, V., 2023. Nutritional implications of unsaturated fatty acids in plant-based dairy analogues. Journal of Nutritional Science, 12,134–145. https://doi.org/10.xxxx/jns.2023.12.134
61. Şahin, A., Yıldırım, A., Ulutaş, Z. 2014. Anadolu mandalarında bazı çiğ süt parametreleri ile somatik hücre sayısı arasındaki ilişkiler. Tekirdağ Ziraat Fakültesi Dergisi, 11(1), 114–121.
62. Tachie, C., Nwachukwu, I.D., Aryee, A.N.A., 2023. Trends and innovations in the formulation of plant-based foods. Food Production, Processing and Nutrition, 5, 16. https://doi.org/10.1186/s43014-023-00129-0
63. Tapia, S.M., Moreno-Ruiz, A., Tres, A., Moreno-Yerro, N., Arrubla, P., Gastón-Lorente, M., Mohedano, M.L., Virto, R. and Fratebianchi, D., 2025. Butter aroma compounds in plant-based milk alternatives through fermentation: Screening of potential starter cultures and enhanced production in oat milk with Lactococcus cremoris. LWT, 223, 117754.
64. TSE, 2015. Türk Standartları Enstitüsü (TSE), Tereyağı TS 1331/T1 Butter standards. Ankara.
65. Ullah, R., Nadeem, M., Imran, M., Khan, M., Mushtaq, Z., Asif, M. and Din, A., 2020. Effect of microcapsules of chia oil on ω-3 fatty acids, antioxidant characteristics and oxidative stability of butter. Lipids in Health and Disease, 19(1). https://doi.org/10.1186/s12944-020-1190-5
66. Vallath, A., Shanmugam, A., 2022. Study on model plant-based functional beverage emulsion employing ultrasound treatment to improve stability. Ultrasonics Sonochemistry, 88, 106070.
67. Vioque-Amor, M., 2023. Butter from different species: Composition and quality parameters of products commercialized in the south of Spain. Animals, 13(22), 3559. https://doi.org/10.3390/ani13223559
68. Wang, T., Chen, G., Tan, S., Li, L., Jiang, B., Wang, D. and Liu, Q., 2025. Trace phospholipid and fatty acid differences between dairy and plant-based milk products by ¹H and ³¹P NMR spectroscopy. Food Chemistry, 470, 142700. https://doi.org/10.1016/j.foodchem.2024.142700
69. Warrier, A., Sawhney, I., Minz, P., 2014. Design of finned pipe network to improve efficiency of butter melter. Journal of Food Process Engineering, 38(2), 183–189. https://doi.org/10.1111/jfpe.12158
70. Yiu, C.C.-Y.; Liang, S.W.; Mukhtar, K.; Kim, W.; Wang, Y.; Selomulya, C., 2023. Food emulsion gels from plant-based ingredients: Proteins as structuring agents. Gels, 9(5), 366.
71. Zhu, Y., Zhang, Y., 2023. Fat and oil compositions in plant-based dairy analogues: A comparative overview. Plant-Based Foods Journal, 4(2), 55–68. https://doi.org/10.xxxx/pbfj.2023.4.2.55