Molecular identification and phylogenetic profiling of keratinolytic bacteria isolated from goose and chicken farms in a cold region of Türkiye
Abstract
The aim of this study was to investigate bacterial diversity in certain goose and poultry farms in Kars, Türkiye a cold climate zone. Feather waste samples rich in organic compounds were collectedfrom multiple farm locations and evaluated for bacterial diversity. A total of 27 bacteria were isolated. 16S rRNA gene sequencing, morphological, physiological, biochemical and molecular techniques were used. Isolates included Escherichia coli, Ralstonia picketti, Pantoea agglomerans, Kosakonia cowanii, Bacillus licheniformis, Bacillus zhangzhouensis, Bacillus pumilus, Exiguobacterium sibiricum, Exiguobacterium artemiae, Macrococcus caseolyticus, Mammaliicoccus vitulinus, Mammaliicoccus sciuri and Rothia nasisuis. Most of the isolates are positive for characteristics such as spore formation, motility and catalase activity. These isolates also showed adaptation to nutrient-rich and varying temperature parameters. Molecular identification results confirmed the results of phylogenetic analyses, showing strong bootstrap support. The identification of resistant bacteria in these keratin-rich regions plays an important role in the control of poultry waste management. These bacteria, which are desirable for biotechnological applications, are also importance in enzyme studies. This study provides valuable information on the functional diversity of microbial communities in goose and poultry farming. It draws attention to the importance of microbial enzymes in the disposal of keratin wastes.
Keywords
16S rRNA, Sequence, Microorganisms, Keratinase, Phylogenetic, Poultry and goose farm microbiota
Supporting Institution
This study was supported by the Scientific Research Projects Coordination Unit of Kafkas University (2021-FM-63).
Project Number
2021-FM-63
Ethical Statement
Peer-review
Externally peer-reviewed.
Conflict of interest
The authors declare that they have no competing, actual, potential or perceived conflict of interest.
Author contribution
The contribution of the authors to the present study is equal. All the authors read and approved the final manuscript.
All the authors verify that the Text and Table are original and that they have not been published before
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