The antioxidant and antialzhemier activities of the Diplotaenia turcica with phytochemical analysis
Abstract
Diplotaenia turcica is an endemic plant that grows in eastern Turkey. This herb is used in herbal cheese, in meals and in traditional therapies. In this study, we aimed to examine some of the biochemical activities of this plant. Liquid chromatography-mass spectrometry (LC-MS) analysis was conducted on hydro alcohol extract of aerial part of DT. This analysis was applied to determine the total phenolic and flavonoid content, antioxidant and anti-alzheimer activities. LC-MS analysis showed that malic acid and quinic acid were found to be major compounds. The key flavonoids detected were hesperidine and rutine. The end of examination, the total amount of phenolic compound of extract was measured as 27.54 μg PEs/mg. And the total flavonoid amount was measured as 7.31 μg KEs/mg. β-carotene-linoleic acid test, DPPH free radical scavenging method, and ABTS cation radical scavenging outcomes were determined as IC50 of 169.71 μg/mL, 164.42 and 68.74 μg/mL, respectively. Cholinesterase inhibition test which indicate of alzheimer's did not inhibit acetylcholinesterase (AChE), but butyrylcholinesterase (BCHE) enzyme inhibition was 76.57%. As a result, further studies are needed in order to use Diplotaenia turcica plant for treatment or support purposes in the health field.
Keywords
Diplotaenia turcica, Antialzhemier, Antioxidant, Activities
Thanks
I would like to thank Mr. Mehmet Fırat for taxonomic examination of the plant and Mr. Mehmet Boğa for his support.
References
- Apak, R., Güçlü, K., Özyürek, M., and Karademir, S. E. (2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of agricultural and food chemistry, 52(26), 7970-7981. Doi: https://doi.org/10.1021/jf048741x
- Boğa, M., Hacıbekiroğlu, I., and Kolak, U. (2011). Antioxidant and anticholinesterase activities of eleven edible plants. Pharmaceutical biology, 49(3), 290-295. Doi: https://www.tandfonline.com/doi/full/10.3109/13880209.2010.517539
- Çölçimen, N., Özdek, U., Değer, Y., Altındağ, F., and Arihan, O. (2019). Investigation of the effects of diplotaenia turcica plant root extract on rat over tissue by histological and stereological methods. Fresenius Environmental Bulletin, 28(4), 2407-2412. Retrieved from https://www.prt-parlar.de/download_feb_2019/
- Ertaş, A., Boğa, M., Yılmaz, M. A., Yeşil, Y., Haşimi, N., Kaya, M. S. e., . . . Kolak, U. (2014). Chemical compositions by using LC-MS/MS and GC-MS and biological activities of Sedum sediforme (Jacq.) Pau. Journal of agricultural and food chemistry, 62(20), 4601-4609. Doi: https://pubs.acs.org/doi/abs/10.1021/jf500067q
- Frankel, E. N., Huang, S.-W., Kanner, J., and German, J. B. (1994). Interfacial phenomena in the evaluation of antioxidants: bulk oils vs emulsions. Journal of agricultural and food chemistry, 42(5), 1054-1059. Doi: https://pubs.acs.org/doi/10.1021/jf00041a001
- Kähkönen, M. P., Hopia, A. I., Vuorela, H. J., Rauha, J.-P., Pihlaja, K., Kujala, T. S., and Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of agricultural and food chemistry, 47(10), 3954-3962. Doi: https://pubs.acs.org/doi/10.1021/jf990146l
- Kaval, I., Behçet, L., and Cakilcioglu, U. (2014). Ethnobotanical study on medicinal plants in Geçitli and its surrounding (Hakkari-Turkey). Journal of Ethnopharmacology, 155(1), 171-184. Doi: https://doi.org/10.1016/j.jep.2014.05.014
- Koleva, I. I., Van Beek, T. A., Linssen, J. P., Groot, A. d., and Evstatieva, L. N. (2002). Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochemical Analysis: An International Journal of Plant Chemical and Biochemical Techniques, 13(1), 8-17. Retrieved from https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.611
- Kosanić, M., Ranković, B., and Dašić, M. (2012). Mushrooms as possible antioxidant and antimicrobial agents. Iranian journal of pharmaceutical research: IJPR, 11(4), 1095. Retrieved from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813146/
- Meydan, İ., Kizil, G., Demir, H., Toptanci, B. C., and Kizil, M. (2020). In vitro DNA damage, protein oxidation protective activity and antioxidant potentials of almond fruit (Amygdalus trichamygdalus) parts (hull and drupe) using soxhlet ethanol extraction. Advances in Traditional Medicine, 1-9. Doi: https://doi.org/10.1007/s13596-020-00491-1
