Hurma (Diospyros kaki), Karayemiş (Prunus lauroceraus) ve Mavi Yemiş (Vaccinium arctostaphylos) Meyvelerinin Doğal Antioksidan Kaynağı Olarak Değerlendirilmesi

Yıl 2021, , 592 - 598, 31.12.2021

Aydın Aytaç Gürdal

https://doi.org/10.35229/jaes.1016793

Öz

Canlıların çoğu hayatlarını devam ettirebilmek için oksijene ihtiyaç duysa hücreler tarafından oksijenin tüketilmesi reaktif oksijen türlerinin (ROT) oluşumuna sebep olarak zehirleyici ve mutasyona sebep olan bir etki gösterebilmektedir. Organizmanın antioksidan sistemi ile arasında bir dengesizlik oluşturan ROT’ların oksidatif strese yol açarak kardiyovasküler hastalıklar, kanser, diyabet, alzaymır ve bazı bağışıklık sorunları ve yaşlanma gibi kronik ve akut hastalıklara sebep olduğu bildirilmektedir. Canlı ortamlarda oksijenin sebep olduğu zararı düşük konsantrasyonda var olduğunda bile geciktirebilen ya da önleyen maddelere antioksidanlar denir. Epidemiyolojik çalışmalar antioksidanca zengin beslenmenin yararlı olduğunu ve hastalıkların önlenmesinde büyük bir etkisi olabileceğini bildirmektedir. Bu sebeple doğal antioksidanlar üzerine birçok araştırma yapılmalıdır.
Bu çalışma Rize ve çevresinde yetişen hurma, karayemiş ve mavi yemiş meyvelerinin farklı yöntemler ile elde edilen ekstraktların antioksidan kapasitesini belirlemek amacıyla yapılmıştır. Bu amaçla Diospyros kaki (hurma), Prunus lauroceraus (karayemiş), Vaccinium arctostaphylos (mavi yemiş) meyveleri 60°C’de kurutulduktan sonra ethanol (40°C), sıcak su (75°C) ve ılık su (40°C) ile ekstraktsiyon işlemine tabi tutulup %2,5 ve %5 oranlarında ekstraktlar elde edilmiştir. Antioksidan etkisinin belirlenmesinde DPPH yöntemi kullanılmıştır. Elde edilen sonuçlara göre antioksidan aktivitesi en fazla mavi yemiş meyvesinin sıcak su ekstraktında, en düşük karayemiş meyvesinin ethanol ile yapılan ekstraktında belirlenmiştir. Ekstrakt konsantrasyonunun arttırılmasının az bir miktarda antioksidan aktiviteyi arttırdığı belirlenmiştir.

Anahtar Kelimeler

Antioksidan, ROS, bitkisel ekstrakt, hurma, karayemiş, mavi yemiş

Evaluation of Diospyros kaki (persimmon), Prunus lauroceraus (cherry laurel) and Vaccinium arctostaphylos (blue berry) Fruits as a Natural Antioxidant Source

Öz

Most living things need oxygen to survive. However, the consumption of oxygen by cells causes the formation of reactive oxygen species (ROS). In this case, oxygen can have a toxic and mutating effect. It is reported that ROS, which create an imbalance between the antioxidant system of the organism and cause oxidative stress, cause chronic and acute diseases such as cardiovascular diseases, cancer, diabetes, Alzheimer's and some immune problems and aging. Antioxidants are substances that can delay or prevent the damage caused by oxygen in living environments, although they exist in low concentrations. Epidemiological studies report that an antioxidant-rich diet is beneficial and can have a great impact on disease prevention. For this reason, a lot of research should be done on natural antioxidants.
For this purpose, Diospyros kaki (persimmon), Prunus lauroceraus (cherry laurel), Vaccinium arctostaphylos (blue berry) fruits are dried at 60°C and then mixed with ethanol (40°C), hot water (75°C) and warm water (40°C). were subjected to the extraction process and extracts were obtained at the rates of 2.5% and 5%. The DPPH method was used to determine the antioxidant effect. According to the results obtained, the highest antioxidant activity was determined in the hot water extract of the blue berry fruit, and the lowest in the ethanol extract of the cherry laurel fruit. It was observed that increasing the extract concentration slightly increased the antioxidant activity.

Anahtar Kelimeler

Antioxidant, ROS, herbal extract, persimmon, cherry laurel, blue berry

Kaynakça

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