Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats

Begüm Boran; Bengi Baycan; Çiğdem Gül; Murat Tosunoğlu

doi:10.35229/jaes.1398305

EN TR

Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats

Öz

Testudo hermanni is considered near threatened globally in the wild. In this study differential leukocyte percentages, nuclear abnormalities, and erythrocyte morphology abnormalities of Testudo hermanni populations from two different habitat types in the Thrace Region were determined to whether habitats have a potential effect on blood cells. A total of 37 tortoises, 18 from an open habitat in Kırklareli/Karakoç, and 19 from an isolated habitat in Edirne/Keşan were studied. According to the results obtained from the Micronucleus Test, which detects genotoxic damage, there was no statistically significant difference between the two habitats. There were statistically significant differences in erythrocyte morphology abnormalities and differential leukocyte count between two populations, such as frequencies of anucleated erythrocyte, nuclear shift, elliptical shape distortion, monocyte and eosinophile percentages. This is the first study examined nuclear abnormalities of Testudo hermanni in Türkiye.

Anahtar Kelimeler

Genotoxicology, Micronucleus Test, Testudo hermanni, Thrace Region

Farklı Habitatlardaki Trakya Tosbağası (Testudo hermanni) Popülasyonlarında Kan Hücre Tipleri ve Anomalileri

Öz

Testudo hermanni, doğada küresel olarak nesli tehlike altında olarak kabul edilmektedir. Bu çalışmada, Trakya Bölgesi’nde iki farklı habitat tipinde bulunan Testudo hermanni popülasyonlarının lökosit formülleri, nüklear anormallikleri ve eritrosit morfolojisi anormallikleri tespit edilerek habitatların kan hücreleri üzerinde potansiyel bir etkiye sahip olup olmadığı belirlenmiştir. Kırklareli/Karakoç’taki açık bir habitattan 18 ve Edirne/Keşan’daki izole bir habitattan 19 olmak üzere toplam 37 kaplumbağa örneği üzerinde çalışılmıştır. Genotoksik hasarı tespit eden Mikronukleus Testi’nden elde edilen sonuçlara göre iki habitat arasında istatistiksel olarak anlamlı bir fark bulunmamıştır. Ancak, çekirdeksiz eritrosit, nüklear kayma ve eliptik şekil bozukluğu gibi eritrosit morfolojisi anormalliklerinde ve monosit ile eozinofil yüzdesinde iki popülasyon arasında anlamlı farklar olduğu tespit edilmiştir. Vücut büyüklükleri ile diğer parametreler arasında korelasyonlar tespit edilmiştir. Bu çalışma, ülkemizde Testudo hermanni’nin nüklear anormalliklerinin incelendiği ilk çalışmadır.

Anahtar Kelimeler

Genotoksikoloji, Mikronükleus Testi, Testudo hermanni, Trakya Bölgesi

Destekleyen Kurum

Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

FDK-2021-3555.

Etik Beyan

Yapılan çalışmalar için Çanakkale Onsekiz Mart Üniversitesi Hayvan Deneyleri Etik Kurulu'ndan (karar no: 2023/01-06) gerekli izinler alınmıştır.

Teşekkür

Bu çalışma Çanakkale Onsekiz Mart Üniversitesi Bilimsel Araştırma Koordinasyon Birimi (Proje Numarası: FDK-2021-3555) tarafından desteklenmiştir.

Kaynakça

Arıkan, H. & Çiçek, K. (2010). Morphology of peripheral blood cells from various species of Turkish herpetofauna. Acta Herpetologica. 5, 179-198. Arıkan, H., Tosunoğlu, M. & Gül, Ç. (2012). Genel Hematoloji Ders Notları ve Uygulama Kılavuzu, Kriter Yayınları, İstanbul.
Aroutiounian, R., Sargsyan, A., Simonyan, A., Hovhannisyan, G. & Arakelyan, M. (2019). Application of the comet assay, micronucleus test and global DNA methylation analysis in Darevskia lizards as a sentinel organism for genotoxic monitoring of soil pollution. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 842, 117-124.
Babini, M.S., Bionda, C., Salas, N.E. & Martino, A.L. (2015). Health status of tadpoles and metamorphs of Rhinella arenarum (Anura, Bufonidae) that inhabit agroecosystems and its implications for land use. Ecotoxicology and Environmental Safety, 118, 118-125. DOI: 10.1016/j.ecoenv.2015.04.017.
Ballard, B.M. & Cheek, R. (eds.) (2003). Exotic animal medicine for the veterinary technician. Wiley and Sons / Blackwell Publishing, New York.
Baran, İ., Avcı, A., Kumlutaş, Y., Olgun, K. & Ilgaz, Ç. (2021). Türkiye amfibi ve sürüngenleri. Palme Yayıncılık, Ankara.
Barni, S., Boncompagni, E., Grosso, A., Bertone, V., Freitas, I., Fasola, M. & Fenoglio, C. (2007). Evaluation of Rana snk esculenta blood cell response to chemical stressors in the environment during the larval and adult phases. Aquatic Toxicology. 81(1), 45e54.
Baycan, B., Boran, B., Gül, Ç. & Tosunoğlu, M. (2022). Clinical Hematology of the Nose-Horned Viper, Vipera ammodytes (Linnaeus 1758). Reptiles & Amphibians, 29(1), 461-469.
Boran, B. (2022). Sarıçay (Çanakkale)’daki Çevresel Parametrelerin Pelophylax bedriagae ve Mauremys rivulata Türleri Üzerinde Hematolojik ve Genotoksikolojik Etkilerinin Belirlenmesi. Yüksek Lisans Tezi, Çanakkale Onsekiz Mart Üniversitesi, Lisansüstü Eğitim Enstitüsü, Çanakkale, Türkiye.
Campbell, T.W. (2004). Hematology of lower vertebrates. In: 55 th Annual Meeting of the American College of Veterinary Pathologists (ACVP) and 39 th Annual Meeting of the American Society of Clinical Pathology (ASVCP). American College of Veterinary Pathologists & American Society for Veterinary Clinical Pathology, Middleton, WI. pp. 1214-1218.
Carballo, M.A. & Mudry, M.D. (2006). Indicadores y marcadores biológicos. In: Genética Toxicológica, pp. 83-108. Mudry, M.D., Carballo, M.A., Eds, De los Cuatro Vientos Editorial, Buenos Aires, Argentina.

Cheylan, M. (2001). Testudo hermanni Gmelin. 1798- Griechische Landschildkröten. In: Fritz. U. (Ed.). Handbuch der Reptilien und Amphibien Europas. Band 3/IIIA: Schildkröten (Testudines). I. (Bataguridae. Testudinidae. Emydidae). Wiebelsheim: Aula-Verlag. pp. 179-289.
Çiçek, K., Arıkan, H., Ayaz, D. & Mermer, A. (2015). Blood cells morphology of Testudo graeca and Testudo hermanni (Testudines: Testudinidae) from Turkey. Biharean Biologist, 9(2), 113-116.
Çördük, N., Hacıoğlu Doğru, N., Gül, Ç. & Tosunoğlu, M. (2022). Determination of the effects of environmental pollution on the Balkan terrapin, Mauremys rivulata (Valenciennes, 1833). Acta Veterinaria Hungarica. 70(3), 245-253.
Dusinska, M., Rundén-Pran, E., Carreira, S. C., & Saunders, M. (2012). Adverse Effects of Engineered Nanomaterials, Chapter 4-Critical Evaluation of Toxicity Tests, 63-83pp. DOI: 10.1016/b978-0-12-386940-1.00004-0.
Fenech, M. (2000). The in vitro micronucleus technique. Mutation Research, 455, 81-95.
Fenech, M., Kirsch-Volders, M., Natarajan, A.T., Surralles, J., Crott, J.W., Parry, J., Norppa, H., Eastmond, D.A., Tucker, J.D. & Thomas, P. (2011). Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells, Mutagenesis, 26(2011) 125-132, DOI: 10.1093/mutage/geq052
Fernández‐Chacón, A., Bertolero, A., Amengual, A., Tavecchia, G., Homar, V. & Oro, D. (2011). Spatial heterogeneity in the effects of climate change on the population dynamics of a Mediterranean tortoise. Global Change Biology, 17(10), 3075-3088.
Freire, E.M.X., Silva, J.M., Navoni, J.A. & Amaral, V.S. (2021). Cytogenetic analysis of nuclear abnormalities in the erythrocytes of gecko lizards (Phyllopezus periosus) collected in a semi-arid region of northeast Brazil: Possible effects of natural background radioactivity. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 865, 503346.
Frossard, A., Coppo, G.C., Lourenço, A.T., Heringer, O.A. & Chippari-Gomes, A.R. (2021). Metal bioaccumulation and its genotoxic effects on eggs and hatchlings of giant Amazon River turtle (Podocnemis expansa). Ecotoxicology, 30(4), 643-657.
Gardner, S.C. & Oberdorster, E. (Eds.). (2005). Toxicology of reptiles. CRC Press.
Guilherme, S., Válega, M., Pereira, M.E., Santos, M.A. & Pacheco, M. (2008). Erythrocytic nuclear abnormalities in wild and caged fish (Liza aurata) along an environmental mercury contamination gradient. Ecotoxicology and Environmental Safety, 70(3), 411-421.
Gül, Ç., Çördük, N., Hacıoğlu-Doğru, N. & Tosunoğlu, M. (2019). Assessment of nuclear abnormalities in erythrocytes of balkan pond turtle Mauremys rivulata (Valenciennes, 1833) (Testudines: Geoemydidae) from the Biga Stream, Çanakkale, Turkey. Acta Zoologica Bulgarica, 71, 219-226.
Guyot, G. & Clobert, J. (1997). Conservation measures for a population of Hermann’s tortoise Testudo hermanni in Southern France bisected by a major highway. Biological Conservation, 79, 251-256.
Hailey, A. (2000). The effects of fire and mechanical habitat destruction on survival of the tortoise Testudo hermanni in northern Greece. Biological Conservation, 92, 321-333.
Hetényi, N., Sátorhelyi, T., Kovács, S. & Hullár, I. (2016). Variations in blood biochemical values in male Hermann’s tortoises (Testudo hermanni). Veterinaria, 65(1), 15-21.
Isani, G., Andreani, G., Carpene, E., Cannavacciuolo, A., Di Girolamo, N. & Ferlizza, E. (2014). Reference values for hematology and plasma biochemistry variables, and protein electrophoresis of healthy Hermann's tortoises (Testudo hermanni ssp.). Veterinary Clinical Pathology, 43(4), 573-583.
Johnsrude, J.D., Raskin, R. E., Hoge, A.Y.A. & Erdos, G.W. (1997). Intraerythrocytic inclusions associated with iridoviral infection in a fer de lance (Bothrops moojeni) snake. Veterinary pathology, 34(3), 235-238.
Josende, M.E., Tozetti, A.M., Alalan, M.T., Filho, V.M., Ximenez, S., Junior, F.M.R. & Martins, S.E. (2015). Genotoxic evaluation in two amphibian species from Brazilian subtropical wetlands. Ecological Indicators, 49, 83-87.
Karaman, S. (2017). Trakya tosbağası (Testudo hermanni)’nin iklimsel parametreler altında yayılış modellemesi. Yüksek Lisans Tezi, Aydın Adnan Menderes Üniversitesi, Fen Bilimleri Enstitüsü, Aydın, Türkiye.
Kirchner, R., Kirchnerová, S., Tirpák, F., Halo Jr, M., Slanina, T., Tokárová, K., ... & Massányi, P. (2023). Biogenic Elements and Heavy Metals in Hermann’s Tortoises-Testudo hermanni: Effect on Serum Biochemistry and Oxidative Status Parameters. Animals, 13(13), 2218.
Latorre, M. A., López González, E. C., Siroski, P.A. & Poletta, G.L. (2015). Basal frequency of micronuclei and hematological parameters in the Side-necked Turtle, Phrynops hilarii (Duméril & Bibron, 1835). Acta Herpetologica, 10(1), 31-37.
Leineweber, C., Stöhr, A. C., Öfner, S., Mathes, K. & Marschang, R. E. (2019). Changes in plasma chemistry parameters in Hermann's tortoises (Testudo hermanni) influenced by season and sex. Journal of Herpetological Medicine and Surgery, 29(3-4), 113-122.
Leineweber, C., Stöhr, A.C., Öfner, S., Mathes, K. & Marschang, R.E. (2021). Plasma capillary zone electrophoresis and plasma chemistry analytes in tortoises (Testudo hermanni, Testudo graeca) and turtles (Trachemys scripta elegans, Graptemys spp.) in fall. Journal of Zoo and Wildlife Medicine, 51(4), 915-925.
Matache, M.L., Rozylowicz, L., Hura, C. & Matache, M. (2006). Organochlorine pesticides-a threat on the hermann’s tortoise perpetuation. Organohalogen Compounds, 68, 728-731.
Mingo, V., Lötters, S. & Wagner, N. (2016). Risk of pesticide exposure for reptile species in the European Union. Environmental Pollution, 215, 164-169.
Natale, G.S., Vera-Candioti, J., de Arcaute, C.R., Soloneski, S., Larramendy, M.L. & Ronco, A.E. (2018). Lethal and sublethal effects of the pirimicarb-based formulation Aficida® on Boana pulchella (Dumeril and Bibron, 1841) tadpoles (Anura, Hylidae). Ecotoxicology and Environmental Safety, 147, 471-479. DOI: 10.1016/j.ecoenv.2017.09.007
Neiffer, D.L., Lydick, D., Burks, K. & Doherty, D. (2005). Hematologic and plasma biochemical changes associated with fenbendazole administration in Hermann's tortoises (Testudo hermanni). Journal of Zoo and Wildlife Medicine, 36(4), 661-672.
Ng, C.T., Li, J.J., Bay, B.H. & Yung, L.Y.L. (2010). Current studies into the genotoxic effects of nanomaterials. Journal of Nucleic Acids, 2010, 1- 12. DOI: 10.4061/2010/947859
Ossana, N.A. & Salibian, A. (2013). Micronucleus test for monitoring the genotoxic potential of the surface water of Lujan River (Argentina) using erythrocytes of Lithobates catesbeianus tadpoles. Ecotoxicology and Environmental Contamination 8, 67e74. DOI: 10.5132/eec.2013.01.010
Özdamar, D. (2014). Trakya Bölgesi’nde Dağılış Gösteren Testudo hermannı (Testudınata; Testudinidae) Popülasyonlarında Plazma Biyokimyasına Ait Parametrelerin Belirlenmesi. Çanakkale Onsekiz Mart Üniversitesi, Fen Bilimleri Enstitüsü, Çanakkale, Türkiye.
Park, S.Y. & Choi, J. (2007). Cytotoxicity, genotoxicity and ecotoxicity assay using human cell and environmental species for the screening of the risk from pollutant exposure. Environment International, 33(6), 817-822.
Peltzer, P.M., Lajmanovich, R.C., Attademo, A.M., Junges, C.M., Cabagna-Zenklusen, M.C., Repetti, M.R., Sigrist, M.E., Beldomenico, H. (2013). Effect of exposure to contaminated pond sediments on survival, development, and enzyme and blood biomarkers in veined treefrog (Trachycephalus typhonius) tadpoles. Ecotoxicology and Environmental Safety, 98, 142-151.
Petersen, A. (2016). Influence of blood storage time and temperature on the evaluation of blood smears from Hermann’s tortoises (Testudo hermanni). Ludwig-Maximilians-Universität, Munich, Germany.
Pollo, F.E., Grenat, P.R., Otero, M.A., Babini, S., Salas, N.E. & Martino, A.L. (2019). Evaluation in situ of genotoxic and cytotoxic response in the diploid/polyploid complex Odontophrynus (Anura: Odontophrynidae) inhabiting agroecosystems. Chemosphere, 216, 306-312.
Rozylowicz, L. & Dobre, M. (2009). Asessment of threatened status of Testudo hermanni boettgeri Mojsisovics, 1889 (Reptilia: Testudines: Testudinidae) population from Romania. Final report for Rufford Small Grant Foundation #49.01.08.
Saquib, Q., Al-Khedhairy, A.A., Siddiqui, M.A., AbouTarboush, F.M., Azam, A. & Musarrat, J. (2012). Titanium dioxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in human amnion epithelial (WISH) cells. Toxicology in Vitro, 26(2), 351-361.
Schaumburg, L.G., Poletta, G.L., Siroski, P.A. & Mudry, M.D. (2012). Baseline values of Micronuclei and Comet Assay in the lizard Tupinambis merianae (Teiidae, Squamata). Ecotoxicology and Environmental Safety, 84, 99- 103.
Schaumburg, L.G., Poletta, G.L., Siroski, P.A. & Mudry, M.D. (2014). Spontaneous genetic damage in the tegu lizard (Tupinambis merianae): the effect of age. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 766, 5-9.
Scope, A., Schwendenwein, I. & Schauberger, G. (2013). Characterization and quantification of the influence of season and gender on plasma chemistries of Hermann’s tortoises (Testudo hermanni, Gmelin 1789). Research in veterinary science, 95(1), 59-68.
Sebbio, C., Carere, C., Nascetti, G., Bellisario, B., Mosesso, P., Cimmaruta, R. & Angeletti, D. (2014). Interspecies variation in DNA damage induced by pollution. Current Zoology, 60, 308- 321.
Şekeroğlu, V. & Atlı-Şekeroğlu, Z. (2011). Genotoksik hasarın belirlenmesinde mikronükleus testi. Türk Hijyen ve Deneysel Biyoloji Dergisi, 68(4), 241- 252.
Silva, J.M., Navoni, J.A., Amaral, V.S. & Freire, E.M.X. (2021). Cytogenetic analysis of nuclear abnormalities in the erythrocytes of gecko lizards (Phyllopezus periosus) collected in a semi-arid region of northeast Brazil: Possible effects of natural background radioactivity. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 865, 503346.
Strunjak-Perovic, I., Lisicic, D., Coz-Rakovac, R., Topic Popovic, N., Jadan, M., Benkovic, V. & Tadic, Z. (2010). Evaluation of micronucleus and erythrocytic nuclear abnormalities in Balkan whip snake Hierophis gemonensis. Ecotoxicology, 19, 1460-1465.
Thrall, A., Baker, D. C., Campbell, T.W., DeNicola, D., Feetman, M.J., Lassen, E.D., Rebar, A. & Weiser, G. (2004). Veterinary hematology and clinical chemistry. Blackwell Publishing. Philadephia, USA.
Tosunoğlu, M., Tok, C.V. & Gül, Ç. (2005). Hematological Values in Hermann’s Tortoise (Testudo hermanni) and Spur-thighed Tortoise (Testudo graeca) from Thrace Region (Turkey). International Journal of Zoological Research, 1(1), 11-14.
Uğurtaş, I.H., Sevinç, M. & Yıldırımhan, H.S. (2003). Erythrocyte size and morphology of some tortoises and turtles from Turkey. Zoological Studies, 42(1), 173-178.
van der Horst, G., Walton, S. & Hofmeyr, M.D. (2013). Accurate automated quantitative imaging of tortoise erythrocytes using the NIS image analysis system. Biotechnic & Histochemistry, 88(5), 242- 249.
Van Dijk, P.P., Corti, C., Mellado, V.P. & Cheylan, M. (2004). Testudo hermanni. The IUCN Red List of Threatened Species 2004: e.T21648A176604335. DOI:10.2305/IUCN.UK.2004.RLTS.T21648A176604 335.en.
Walton, S. & Hofmeyr, M. (2017). Erythrocyte morphology of the Critically Endangered Geometric Tortoise Psammobates geometricus: effects of season, sex and age. African Journal of Herpetology, 66(1), 39-52.
Willemsen, R.E. & Hailey, A. (2003). Sexual dimorphism of body size and shell shape in European tortoises. Journal of Zoology, London 260, 353-365.
Zhang, F., Gu, H. & Li, P. (2011). A review of chelonian hematology. Asian Herpetological Research, 2(1), 12-20.
Zúñiga-González, G., Torres-Bugarın, O., LunaAguirre, J., González-Rodrıguez, A., ZamoraPerez, A.A., Gómez-Meda, B.C., ... & GallegosArreola, M.P. (2000). Spontaneous micronuclei in peripheral blood erythrocytes from 54 animal species (mammals, reptiles and birds): Part two. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 467(1), 99-103.

Ayrıntılar

Birincil Dil

İngilizce

Konular

Hayvan Bilimi (Diğer)

Bölüm

Araştırma Makalesi

Yazarlar

Begüm Boran ^*
0000-0002-3069-7780
Türkiye

Bengi Baycan
0000-0002-2758-6166
Türkiye

Çiğdem Gül
0000-0003-4736-2677
Türkiye

Murat Tosunoğlu
0000-0002-9764-2477
Türkiye

Erken Görünüm Tarihi

22 Mart 2024

Yayımlanma Tarihi

31 Mart 2024

Gönderilme Tarihi

30 Kasım 2023

Kabul Tarihi

19 Mart 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 9 Sayı: 1

DOI

https://doi.org/10.35229/jaes.1398305

IZ

https://izlik.org/JA77YN56FJ

APA

Boran, B., Baycan, B., Gül, Ç., & Tosunoğlu, M. (2024). Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats. Journal of Anatolian Environmental and Animal Sciences, 9(1), 110-118. https://doi.org/10.35229/jaes.1398305

AMA

1.Boran B, Baycan B, Gül Ç, Tosunoğlu M. Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats. Journal of Anatolian Environmental and Animal Sciences. 2024;9(1):110-118. doi:10.35229/jaes.1398305

Chicago

Boran, Begüm, Bengi Baycan, Çiğdem Gül, ve Murat Tosunoğlu. 2024. “Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats”. Journal of Anatolian Environmental and Animal Sciences 9 (1): 110-18. https://doi.org/10.35229/jaes.1398305.

EndNote

Boran B, Baycan B, Gül Ç, Tosunoğlu M (01 Mart 2024) Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats. Journal of Anatolian Environmental and Animal Sciences 9 1 110–118.

IEEE

[1]B. Boran, B. Baycan, Ç. Gül, ve M. Tosunoğlu, “Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats”, Journal of Anatolian Environmental and Animal Sciences, c. 9, sy 1, ss. 110–118, Mar. 2024, doi: 10.35229/jaes.1398305.

ISNAD

Boran, Begüm - Baycan, Bengi - Gül, Çiğdem - Tosunoğlu, Murat. “Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats”. Journal of Anatolian Environmental and Animal Sciences 9/1 (01 Mart 2024): 110-118. https://doi.org/10.35229/jaes.1398305.

JAMA

1.Boran B, Baycan B, Gül Ç, Tosunoğlu M. Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats. Journal of Anatolian Environmental and Animal Sciences. 2024;9:110–118.

MLA

Boran, Begüm, vd. “Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats”. Journal of Anatolian Environmental and Animal Sciences, c. 9, sy 1, Mart 2024, ss. 110-8, doi:10.35229/jaes.1398305.

Vancouver

1.Begüm Boran, Bengi Baycan, Çiğdem Gül, Murat Tosunoğlu. Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats. Journal of Anatolian Environmental and Animal Sciences. 01 Mart 2024;9(1):110-8. doi:10.35229/jaes.1398305

JAES, 2016- 11.yıl

Blood Cell Types and Abnormalities in Free Ranging Hermann’s Tortoise (Testudo hermanni) Populations from Different Habitats

Öz

Anahtar Kelimeler

Farklı Habitatlardaki Trakya Tosbağası (Testudo hermanni) Popülasyonlarında Kan Hücre Tipleri ve Anomalileri

Öz

Anahtar Kelimeler

Destekleyen Kurum

Proje Numarası

Etik Beyan

Teşekkür

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Erken Görünüm Tarihi

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster