Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex'in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler

Şule Tatar; Osman Serdar; Nuran Cıkcıkoğlu Yıldırım

doi:10.35229/jaes.542705

EN TR

Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex'in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler

Öz

Bu çalışmada, kongo kırmızısına maruz bırakılan G ammarus pulex'de Glutatyon S Transferaz ve Sitokrom 1A1 enzimleri ile Malondialdehit seviyelerindeki değişimin araştırılması amaçlanmaktadır. G. pulex, 96 saat boyunca 20, 10, 5 ppm kongo kırmızısı içeren sentetik çözeltilere maruz bırakılmıştır. Glutatyon S Transferaz ve Sitokrom 1A1 enzim aktiviteleri ELISA kiti kullanılarak, malondialdehit seviyeleri ise spektrofotometrik olarak belirlenmiştir. Glutatyon S Transferaz aktiviteleri tüm uygulama gruplarında 24 ve 96 saat boyunca kontrole kıyasla artmıştır (p<0.05). Kongo kırmızısına maruz bırakıldıktan sonra tüm uygulama gruplarında Sitokrom 1A1 aktivitesi 24 ve 96 saat boyunca kontrole kıyasla azalmıştır (p<0.05). Malondialdehit seviyeleri kongo kırmızısına maruziyetinden sonra tüm gruplarda 24 saat boyunca kontrole kıyasla artmış (p<0.05), 96 saat sonunda A grubunda artmış, B ve C gruplarında düşmüştür (p<0.05). Bulgularımız, kongo kırmızısının reaktif oksijen türleri üreterek oksidatif strese neden olabileceğini göstermektedir. Sonuç olarak, antioksidan enzimler ve Malondialdehit seviyelerindeki değişiklikler, kongo kırmızısının çevresel toksisitesinin değerlendirilmesinde potansiyel hassas biyobelirteçler olarak kullanılabileceğini göstermektedir.

Anahtar Kelimeler

G. pulex,Glutatyon S Transferaz,Sitokrom 1A1,Malondialdehit

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Şule Tatar ^*
0000-0001-8962-0107
Türkiye

Osman Serdar Bu kişi benim
0000-0003-1744-8883
Türkiye

Nuran Cıkcıkoğlu Yıldırım
0000-0003-3975-6705
Türkiye

Yayımlanma Tarihi

31 Ağustos 2019

Gönderilme Tarihi

21 Mart 2019

Kabul Tarihi

25 Nisan 2019

Yayımlandığı Sayı

Yıl 2019 Cilt: 4 Sayı: 2

DOI

https://doi.org/10.35229/jaes.542705

IZ

https://izlik.org/JA57ZW42WR

APA

Tatar, Ş., Serdar, O., & Cıkcıkoğlu Yıldırım, N. (2019). Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex’in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler. Journal of Anatolian Environmental and Animal Sciences, 4(2), 76-81. https://doi.org/10.35229/jaes.542705

AMA

1.Tatar Ş, Serdar O, Cıkcıkoğlu Yıldırım N. Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex’in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler. Journal of Anatolian Environmental and Animal Sciences. 2019;4(2):76-81. doi:10.35229/jaes.542705

Chicago

Tatar, Şule, Osman Serdar, ve Nuran Cıkcıkoğlu Yıldırım. 2019. “Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex’in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler”. Journal of Anatolian Environmental and Animal Sciences 4 (2): 76-81. https://doi.org/10.35229/jaes.542705.

EndNote

Tatar Ş, Serdar O, Cıkcıkoğlu Yıldırım N (01 Ağustos 2019) Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex’in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler. Journal of Anatolian Environmental and Animal Sciences 4 2 76–81.

IEEE

[1]Ş. Tatar, O. Serdar, ve N. Cıkcıkoğlu Yıldırım, “Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex’in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler”, Journal of Anatolian Environmental and Animal Sciences, c. 4, sy 2, ss. 76–81, Ağu. 2019, doi: 10.35229/jaes.542705.

ISNAD

Tatar, Şule - Serdar, Osman - Cıkcıkoğlu Yıldırım, Nuran. “Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex’in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler”. Journal of Anatolian Environmental and Animal Sciences 4/2 (01 Ağustos 2019): 76-81. https://doi.org/10.35229/jaes.542705.

JAMA

1.Tatar Ş, Serdar O, Cıkcıkoğlu Yıldırım N. Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex’in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler. Journal of Anatolian Environmental and Animal Sciences. 2019;4:76–81.

MLA

Tatar, Şule, vd. “Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex’in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler”. Journal of Anatolian Environmental and Animal Sciences, c. 4, sy 2, Ağustos 2019, ss. 76-81, doi:10.35229/jaes.542705.

Vancouver

1.Şule Tatar, Osman Serdar, Nuran Cıkcıkoğlu Yıldırım. Kongo Kırmızısına Maruz Bırakılan Tatlı Su Amphipodu Gammarus Pulex’in Antioksidan ve Detoksifikasyon Sistemindeki Değişiklikler. Journal of Anatolian Environmental and Animal Sciences. 01 Ağustos 2019;4(2):76-81. doi:10.35229/jaes.542705