Genetic analysis of Carasobarbus Karaman, 1971 (Actinopterygii Cyprinidae) in Anatolia
Abstract
We sequenced the complete mitochondrial cytochrome b gene (1141 bp) of 70 individuals from ten populations of three Carasobarbus species in Anatolia. Totally eight haplotypes were identified. The intraspecies genetic distance ranged from 0.00% to 0.21%, while it varied from 2.6% to 9.0% for interspecies. Except for Gaziantep samples with low sample size, high haplotype diversity (Hd= 0,590-0,833) and low nucleotide diversity (Pi= 0,05-0,65) values can be explained by the presence of small populations sensitive to genetic drift and founder effects. Phylogenetic analyses constructed with neihgbour joining, maximum likelihood and maximum parsimony generated similar topologies supported by high bootstrap values. Phylogenetic tree topologies showed that the C. apoensis haplotype was located in the C. luteus species. Therefore, the validity of species status of C. apoensis should be checked morphologically. On the other hand, since the Kahta population in C. luteus has a remarkably high genetic diversity, it must be re-evaluated morphologically. The tectonic uplift of the Anatolian Plateau between the African and European plates during the Pliocene period may have probably prevented the presence of Carasobarbus in the west of the Anatolian diagonal.
Keywords
Anatolia , Carasobarbus , Phylogeny , mtDNA
Kaynakça
- Abdoli, A. (2000). The Inland Water Fishes of Iran. Iranian Museum of Nature and Wildlife, Tehran. 378 pp. In Farsi.
- Anderson, S., Bankler, A.T. & Barrel, B.G. (1981). Sequence and organization of the human mitochondrial genome. Nature, 290, 457-465.
- Ansell, S.W., Stenlien, H.K., Grundmann, M., Russell, S.J., Koch, M.A., Schneider, H. & Vogel, J.C. (2011). The importance of Anatolian mountains as the cradle of global diversity in Arabis alpina, a key arctic – alpine species. Annals of Botany, 108, 241–252.
- Aranishi, F., Okimoto, T. & Izumi, S. (2005). Identification of gadoid species (pisces, Gadidae) by PCR‐RFLP analysis. Journal of Applied Genetics, 46, 69–73.
- Borkenhagen, K. & Krupp, F. (2013). Taxonomic revision of the genus Carasobarbus Karaman, 1971 (Actinopterygii, Cyprinidae). ZooKeys, 339, 1–53.
- Borkenhagen, K. (2005). Systematik und Zoogeographie der “großschuppigen Barben” des Vorderen Orients. ChristianAlbrechts-Universität, Kiel
- Borkenhagen, K. (2017). Molecular phylogeny of the tribe Torini Karaman, 1971 (Actinopterygii: Cypriniformes) from the Middle East and North Africa. Zootaxa, 4236, 291–301.
- Borkenhagen, K., Esmaeili, HR., Mohsenzadeh S. & Shahryari, F.G.A. (2011). The molecular systematics of the Carasobarbus species from Iran and adjacent areas, with comments on Carasobarbus albus (Heckel, 1843). Environmental Biology of Fishes, 91, 327–335. Doi: 10.1007/s10641-011-9787-1.
- Briolay, J., Galtier, N., Brito, R.M. & Bouvet, Y. (1998). Molecular phylogeny of Cyprinidae inferred from cytochrome b DNA sequences. Molecular Phylogenetics and Evolution, 9, 100–108. Doi: 10.1006/mpev.1997.0441.
- Cantatore, P., Roberti, M., Pesole, G., Ludovico, A., Milella, F., Gadaleta, M. N. & Saccone, C. (1994). Evolutionary analysis of cytochrome b sequences in some perciformes evidence for a slower rate of evolution than in mammals. Journal of Molecular Evolution, 39, 589–597.