Parvovirus ile Enfekte Köpeklerde Nötrofil/Lenfosit, Monosit/Lenfosit ve Platelet/Lenfosit Oranlarının Belirlenmesi

Yıl 2021, , 585 - 591, 31.12.2021

Didem Pekmezci , Zeynep Nurselin Çolak

https://doi.org/10.35229/jaes.993377

Cited By: 2

Öz

Nötrofil/lenfosit oranı (NEU/LYM), monosit/lenfosit oranı (MONO/LYM), ve platelet/lenfosit oranı (PLT/LYM) inflamasyonunun ucuz, tekrar kullanılabilen biyobelirteçlerini oluşturmaktadır. Bu çalışma ilk defa parvovirus enfeksiyonu (PVE) şekillenmiş köpeklerde NEU/LYM, MONO/LYM ve PLT/LYM değerlerini ortaya koymaktadır. Çalışmanın amacını ise tedavi öncesi PVE ile pozitif köpeklerin NEU/LYM, MONO/LYM ve PLT/LYM değerlerinin, sağlıklı kontrollere göre karşılaştırılması oluşturmuştur. On sekiz sahipli, 2-8 aylık, PVE pozitif köpek, çalışma grubunu oluşturmuştur. Yedi sahipli, aynı yaş aralığında sağlıklı köpek ise kontrol grubunu oluşturmuştur. Gruplar arasında tedavi öncesi NEU/LYM, MONO/LYM karşılaştırılmasında PVE pozitif grubu ortalama NEU (P<0,001), LYM (P<0,05) değerleri kontrol grubuna istatistiksel olarak düşük bulunmuştur. Bununla birlikte, PVE pozitif grubunda tedavi öncesi ortalama PLT/LYM seviyeleri kontrol grubuna göre istatistiksel olarak yüksek (P<0,001) tespit edilmiştir. PVE pozitif ve kontrol gruplarında ortalama PLT/LYM düzeyleri ise sırasıyla 1120,94 ve 135,26 olarak bulunmuştur. Sonuç olarak, bu ön çalışma PLT/LYM oranın, PVE ile doğal olarak enfekte olmuş köpeklerde kolay, uygun maliyetli, objektif ve invazif olmayan yeni bir prognostik belirteç adayı olabileceğini göstermektedir. NEU/LYM, MONO/LYM, PLT/LYM ile ilgili çalışmaların kısıtlı varlığına rağmen, özellikle veteriner hekimlikte bulaşıcı hastalıklarda daha ileri çalışmalara ihtiyaç duyulmaktadır.

Anahtar Kelimeler

Nötrofil/lenfosit oranı, Monosit/lenfosit oranı, Platelet/lenfosit oranı, Parvovirus, Köpek

Destekleyen Kurum

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Proje Numarası

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Teşekkür

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Determination of Neutrophil/Lymphocyte, Monocyte/Lymphocyte and Platelet/Lymphocyte Ratios in Dogs with Occurring Parvovirus Infection

Öz

Neutrophil/lymphocyte ratio (NEU/LYM), monocyte/lymphocyte ratio (MONO/LYM), and platelet/lymphocyte ratio (PLT/LYM) are inexpensive and reproducible biomarkers of inflammation. This is the first study that explores NEU/LYM, MONO/LYM, and PLT/LYM in dogs with Parvovirus infection (PVI). This study aims to determine whether the pretreatment NEU/LYM, MONO/LYM, and PLT/LYM differs in dogs with PVI against healthy controls. Eighteen client-owned dogs aged 2-8 months that were found positive with PVI were enrolled as the study group. Seven client-owned, healthy dogs of the same age formed the control group. Pretreatment NEU/LYM, MONO/LYM, and PLT/LYM were compared between groups. The pretreatment mean NEU (P< 0.001) and LYM (P<0.05) levels were found to be significantly lower in the study group when compared to the control group. However, pretreatment mean PLT/LYM levels in the PVI positive group was significantly higher (P<0.001) than in the control group. The mean PLT/LYM levels in the PVI positive and control groups were found as 1120.94 and 135.26, respectively. In summary, this preliminary study demonstrates that pretreatment PLT/LYM in dogs with PVI positive is an easy, cost-effective, objective, and non-invasive biomarker that could be the new prognostic marker candidates in dogs naturally infected with PVI. Although limited studies on NEU/LYM, MONO/LYM, PLT/LYM are existed, especially further studies in veterinary medicine with infectious diseases are needed.

Anahtar Kelimeler

Neutrophil/lymphocyte ratio, Monocyte/lymphocyte ratio, Platelet/lymphocyte ratio, Parvovirus, Dog

Proje Numarası

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Kaynakça

• Apaa, T.T., Daly, J.M. & Tarlinton, R.E. (2016). Canine parvovirus (CPV-2) variants circulating in nigerian dogs. Veterinary Record Open, 3.
• Ayyıldız, M. & Tekin, M.E. (2021). Köpeklerin parvoviral enterit hastalığı teşhisinde bazı hemogram değerlerinin ROC eğrisi ile incelenmesi. Eurasian Journal of Veterinary Sciences, 37(2), 101-108.
• Benvenuti, E., Pierini, A., Gori, E., Lucarelli, C., Lubas, G. & Marchetti, V. (2020). Neutrophil-to-lymphocyte ratio (nlr) in canine inflammatory bowel disease (IBD). Veterinary Sciences, 7(3),141.
• Burton, A.G., Harris, L.A., Owens, S.D., Jandrey, K.E. (2013). The prognostic utility of degenerative left shifts in dogs. Journal of Veterinary Internal Medicine, 27, 1517-1522.
• Cavalli, A., Desario, C., Kusi, I., Mari, V., Lorusso, E., Cirone, F. Kumbe, I., Colaianni, M.L., Buonavoglia, D. & Decaro, N. (2014). Detection and genetic characterization of canine parvovirus and canine coronavirus strains circulating in district of Tirana in Albania. Journal of Veterinary Diagnostic Investigation, 26, 563-566.
• Chandrashekara, S., Mukhtar Ahmad, M., Renuka, P., Anupama, K.R. & Renuka, K. (2017). Characterization of neutrophil-to-lymphocyte ratio as a measure of inflammation in rheumatoid arthritis. International Journal of Rheumatic Diseases, 20(10), 1457-1467.
• Çakır, K. & Pekmezci, D. (2019). Borrelia burgdorferi ile doğal enfekte köpeklerin nötrofil-lenfosit, monosit- lenfosit ve platelet-lenfosit oranlarının belirlenmesi. Etlik Veteriner Mikrobiyoloji Dergisi, 30(1), 58-63.
• Decaro, N. & Buonavoglia, C. (2012). Canine parvovirus—a review of epidemiological and diagnostic aspects, with emphasis on type 2c. Veterinary Microbiology, 155, 1-12.
• Decaro, N., Cirone, F., Desario, C., Elia, G., Lorusso, E., Colaianni, M.L., Martella, V. & Buonavoglia, C. (2009). Severe parvovirus in a 12-year-old dog that had been repeatedly vaccinated. Veterinary Record, 164, 593-595.
• Decaro, N., Desario, C., Billi, M., Mari, V., Elia, G., Cavalli, A., Martella, V., & Buonavoglia, C. (2011). Western European epidemiological survey for parvovirus and coronavirus infections in dogs. The Veterinary Journal, 187, 195-199.
• Dik, I. & Şimşek, A. (2021). Comparison of different diagnostic methods in detection of canine parvovirus infection. Eurasian Journal of Veterinary Sciences, 37(2), 76-81.
• Dossin, O., Rupassara, S.I., Weng, H.Y., Williams, D.A., Garlick, P.J. & Schoeman, J.P. (2011). Effect of parvoviral enteritis on plasma citrulline concentration in dogs. Journal of Veterinary Internal Medicine, 25(2): 215-221.
• Duijvestijn, M., Mughini-Gras, L., Schuurman, N., Schijf, W., Wagenaar, J.A. & Egberink, H. (2016). Enteropathogen infections in canine puppies: (co-) occurrence, clinical relevance and risk factors. Veterinary Microbiology, 195, 115-122.
• Duque-García, Y., Echeverri-Zuluaga, M., Trejos-Suarez, J. & Ruiz-Saenz, J. (2017). Prevalence and molecular epidemiology of canine parvovirus 2 in diarrheic dogs in Colombia, South America: a possible new cpv-2a is emerging? Veterinary Microbiology, 201, 56-61.
• Filipov, C., Desario, C., Patouchas, O., Eftimov, P., Gruichev, G., Manov, V., Filipov, G., Buonavoglia, C. & Decaro, N. (2016). A ten-year molecular survey on parvoviruses infecting carnivores in Bulgaria. Transboundary and Emerging Diseases, 63, 460-464.
• Gargari, S. (2015). Gastroenteritis semptomlu köpeklerde canine parvovirus tip-2’nin tespiti ve moleküler karakterizasyonu. Ankara Üniversitesi Sağlık Bilimleri Enstitüsü. Ankara, Türkiye.
• Goddard, A. & Leisewitz, A.L. (2010). Canine parvovirus. Veterinary Clinics of North America: Small Animal Practice, 40, 1041-1053.
• Gori, E., Pierini, A., Lippi, I., Lubas, G. & Marchetti, V. (2021) Leukocytes ratios in feline systemic ınflammatory response syndrome and sepsis: a retrospective analysis of 209 cases. Animals, 6, 1644.
• Hodgson, N., Llewellyn, E.A. & Schaeffer, D.J. (2018). Utility and Prognostic Significance of Neutrophil-to-Lymphocyte Ratio in Dogs with Septic Peritonitis. Journal of the American Animal Hospital Association, 54, 351-359.
• Hoskins, J.D. (2001). Canine viral enteritis, In: Ettinger, S.J. (Ed), Pocket Companion to Text Book of Veterinary Internal Medicine. 5th ed., 214-218p, WB Saunders Company, Philadelphia, USA.
• Hoskins, JD. (1997). Update on canine parvoviral enteritis. Veterinary Medicine, 92(8), 694-709.
• Huang, Z., Fu, Z., Huang, W. & Huang, K. (2020). Prognostic value of neutrophil-to-lymphocyte ratio in sepsis: A meta-analysis. American Journal of Emergency Medicine, 38, 641-647.
• Hwang, S.Y., Shin, T.G., Jo, I.J., Jeon, K., Suh, G.Y., Lee, T.R., Yoon, H., Cha, W.C. & Sim, M.S. (2017). Neutrophil-to-lymphocyte ratio as a prognostic marker in critically-ill septic patients. American Journal of Emergency Medicine, 35, 234-239.
• Jenne, C.N. & Kubes, P. (2015). Platelets in inflammation and infection. Platelets, 26(4), 286-292.
• Kalli, I., Leontides, L.S., Mylonakis, M.E., Adamama-Moraitou, K., Rallis, T. & Koutinas, A.F. (2010). Factors affecting the occurrence, duration of hospitalization and final outcome in canine parvovirus infection. Research in Veterinary Science, 89(2), 174-178.
• Li, H., Zhou, Y., Ma, Y., Han, S. & Zhou, L. (2017). The prognostic value of the platelet-to- lymphocyte ratio in acute coronary syndrome: a systematic review and meta-analysis. Kardiologia Polska, 75(7), 666-673.
• Ling, M., Norris, J.M., Kelman, M. & Ward, M.P. (2012). Risk factors for death from canine parvoviral related disease in Australia. Veterinary Microbiology, 158, 280-290.
• Liu, Y., Zheng, J., Zhang, D. & Jing, L. (2019). Neutrophil-lymphocyte ratio and plasma lactate predict 28-day mortality in patients with sepsis. Journal of Clinical Laboratory Analysis, 33, e22942.
• McClure, V., van Schoor, M., Thompson, P.N., Kjelgaard-Hansen, M. & Goddard, A. (2013). Evaluation of the use of serum C-reactive protein concentration to predict outcome in puppies infected with canine parvovirus. Journal of the American Veterinary Medical Association, 243(3), 361-366.
• Min, G.T., Li, Y.M., Yao, N., Wang, J., Wang, H.P. & Chen, W. (2017). The pretreatment neutrophil-lymphocyte ratio may predict prognosis of patients with liver Cancer: meta-analysis. Clinical Transplantation, 32(1).
• Mohr, A.J., Leisewitz, A.L., Jacobson, L.S., Steiner, J.M., Ruaux, C.G. & Williams, D.A. (2003). Effect of early enteral nutrition on intestinal permeability, intestinal protein loss, and outcome in dogs with severe parvoviral enteritis. Journal of Veterinary Internal Medicine, 17, 791-798.
• Mylonakis, M.E., Kalli, I. & Rallis, T.S. (2016). Canine parvoviral enteritis: an update on the clinical diagnosis, treatment, and prevention. Veterinary Medicine, 7, 91-100.
• Naess, A., Nilssen, S.S., Mo, R., Eide, G.E. & Sjursen, H. (2017). Role of neutrophil to lymphocyte and monocyte to lymphocyte ratios in the diagnosis of bacterial infection in patients with fever. Infection, 45, 299-307.
• Neumann, S. (2021). Neutrophil-to-lymphocyte and platelet-to-lymphocyte ratios in dogs and cats with acute pancreatitis. Veterinary Clinical Pathology, 50(1), 45-45.
• Ni, J., Wang, H., Li, Y., Shu, Y. & Liu, Y. (2019). Neutrophil to lymphocyte ratio (NLR) as a prognostic marker for in-hospital mortality of patients with sepsis: A secondary analysis based on a single-center, retrospective, cohort study. Medicine, 98, e18029.
• O'Sullivan, G., Durham, P.J., Smith, J.R. & Campbell, R.S. (1984). Experimentally induced severe canine parvoviral enteritis. Australian Veterinary Journal, 61(1),1-4.
• Otto, C.M., Drobatz, K.J. & Soter, C. (1997). Endotoxemia and tumor necrosis factor activity in dogs with naturally occurring parvoviral enteritis. Journal of Veterinary Internal Medicine, 11(2): 65-70.
• Pekmezci, D. & Çakır, K. (2020). Doğal parvovirus ile enfekte köpeklerde tedavi öncesi serum albuminin globuline oranının değerlendirilmesi. Anadolu Çevre ve Hayvancılık Dergisi, 5(2), 118-124.
• Pierini, A., Gori, E., Lippi, I., Ceccherini, G., Lubas, G., Marchetti, V. (2019). Neutrophil-to-lymphocyte ratio, nucleated red blood cells and erythrocyte abnormalities in canine systemic inflammatory response syndrome. Research in Veterinary Science, 126, 150-154.
• Pierini, A., Gori, E., Lippi, I., Lubas, G. & Marchetti, V. (2020) Are leukocyte and platelet abnormalities and complete blood count ratios potential prognostic markers in canine sepsis? Frontiers in Veterinary Science, 7, 578846.
• Prittie, J. (2004). Canine parvoviral enteritis: a review of diagnosis, management, and prevention. Journal of Veterinary Internal Medicine, 14, 167-176.
• Rejec, A., Butinar, J., Gawor, J. & Petelin, M. (2017). Evaluation of complete blood count indices (NLR, PLR, MPV/PLT, and PLCRi) in healthy dogs, dogs with periodontitis and dogs with oropharyngeal tumors as potential biomarkers of systemic inflammatory response. Journal of Veterinary Dentistry, 34, 231-240.
• Rossi, S., Basso, M., Strippoli, A., Schinzari, G., D’Argento, E., Larocca, M., Cassano, A. & Barone, C. (2017). Are markers of systemic inflammation good prognostic indicators in colorectal cancer? Clin Colorectal Cancer, 16, 264-274.
• Schoeman, J.P. & Herrtage, M.E. (2008). Serum thyrotropin, thyroxine and free thyroxine concentrations as predictors of mortality in critically ill puppies with parvovirus infection: a model for human paediatric critical illness? Microbes and Infection, 10(2), 203-207.
• Schoeman, J.P., Goddard, A. & Herrtage, M.E. (2007). Serum cortisol and thyroxine concentrations as predictors of death in critically ill puppies with parvoviral diarrhea. Journal of the American Veterinary Medical Association, 231(10), 1534-1539.
• Schoeman, J.P., Goddard, A. & Leisewitz, A.L. (2013). Biomarkers in canine parvovirus enteritis. New Zealand Veterinary Journal, 61, 217-222.
• Shi, L., Qin, X., Wang, H., Xia, Y., Li, Y., Chen, X., Shang, L., Tai, Y.T., Feng, X., Acharya, P., Acharya, C., Xu, Y., Deng, S., Hao, M., Zou, D., Zhao, Y., Ru, K., Qiu, L. & An, G. (2017). Elevated neutrophil-to-lymphocyte ratio and monocyte-to-lymphocyte ratio and decreased platelet-to-lymphocyte ratio are associated with poor prognosis in multiple myeloma. Oncotarget, 8(12), 18792-18801.
• Smith-Carr, S., Macintire, D.K. & Swango, L.J. (1997). Canine parvovirus. Part I. Pathogenesis and vaccination. Compendium on Continuing Education for the Practising Veterinarian,19(2), 125-133.
• Summers, C., Rankin, S.M., Condliffe, A.M., Singh, N., Peters, A.M. & Chilvers, E.R. (2010). Neutrophil kinetics in health and disease. Trends in Immunology, 31(8), 318-324.
• Troìa, R., Agnoli, C., Calipa, S., Segalina, S., Murgia, E., Gruarin, M., Dondi, F. & Giunti, M. (2017). Evaluation of the delta neutrophil index from an automated blood cell analyser in septic dogs. The Veterinary Journal, 230, 13-19.
• Wang, Y., Fuentes, H.E., Attar, B.M., Jaiswal, P. & Demetria, M. (2017). Evaluation of the prognostic value of neutrophil to lymphocyte ratio in patients with hypertriglycer- idemia-induced acute pancreatitis. Pancreatology, 17(6), 893-897.
• Yao, C., Liu, X. & Tang, Z. (2017). Prognostic role of neutrophil-lymphocyte ratio and pla- telet-lymphocyte ratio for hospital mortality in patients with AECOPD. International Journal of Chronic Obstructive Pulmonary Disease, 12, 2285-2290.
• Yilmaz, Z. & Senturk, S. (2007). Characterization of lipid profiles in dogs with parvoviral enteritis. Journal of Small Animal Practice, 48, 643-650.
• Zhao, Z., Liu, H., Ding, K., Peng, C., Xue, Q., Yu, Z. & Xue, Y. (2016). Occurrence of canine parvovirus in dogs from Henan province of China in 2009–2014. BMC Veterinary Research, 12.