Yıl 2020, Cilt 10 , Sayı 1, Sayfalar 70 - 74 2020-03-31

Ratlarda Deneyse Olarak Oluşturulan İnfilamasyonla İlişkili Timpanoskleroza Alfa Lipoik Asidin Etkisi
The Application of Lipoic Acid in Experimentally Induced Tympanosclerosis in Rats

Fatih TURAN [1] , İlyas SAYAR [2] , İsmail SALCAN [3]


Amaç: Timpanoskleroz timpanik membran ve orta kulak mukozasının sklerotik ve hiyalinize değişikliği ile giden anormal bir doku reaksiyonudur. Bir antioksidan olan alfa lipoik asidin, inflamasyonla ile ilişkili timpanosklerozda basit ve etkili bir tedavi biçimini temsil edebileceğini varsaydık. Çalışmanın amacı, alfa lipoik asidin sıçanlarda deneysel olarak indüklenen infilamasyon ile ilşkili timpanoskleroz üzerindeki etkilerini araştırmaktı.
Materyal Metod: Çalışmamızda 20 adet Wistar albino sıçan kullanıldı. Sıçanlar rastgele olarak onarlı iki gruba ayrıldı. 1. gruba ALA 50 mg/kg yedi gün boyunca günde bir defa intraperitoneal uygulandı. 2. gruba salin aynı miktarda yedi gün boyunca günde bir defa intraperitoneal uygulandı. Histopatolojik olarak değerlendirmemizde inflamasyon, fibrozis ve lamina propria kalınlığı değerlendirildi.
Bulgular: Orta kulakta enflamasyon, fibrozis ve timpanik membran lamina propria kalınlığı değerlendirildiğinde, çalışma grubunda kontrol gurubuna göre daha az olduğu değerlendirildi.
Sonuç: Antiokstidan özellik gösteren alfa lipoik asit’in deneysel olarak oluşturulmuş inflamasyonla ile ilişkili timpanosklerozu önleyebileceği görüldü.

Abstract

Background/Aims:Tympanosclerosis is an abnormal tissue reaction resulting in sclerotic and hyalinization changes in the tympanic membrane and middle ear.  We hypothesized that alpha lipoic acid, an antioxidant, may represent a simple and effective form of treatment in inflammattion-related tympanosclerosis. The purpose of the study was to investigate the effects of alpha lipoic acid on experimentally induced inflammattion-related tympanosclerosis in rats.

Methods:Twenty albino Wistar rats were randomly divided into two equal groups. The first (study) group was administered 50 mg/kg alpha lipoic acid by the intraperitoneal route once daily for 7 days. The second group was administered the same saline solution intraperitoneally once daily for 7 days. Inflammation, fibrosis and lamina propria thickness were assessed at the subsequent histopathological examination.

Results:In the study group, significantly less middle ear inflammation and fibrosis developed and the tympanic membrane lamina propria was thinner compared to the control group. 

Conclusions: Alpha lipoic acid was found capable of preventing experimentally induced inflammattion-related tympanosclerosis with its antioxidant characteristics.





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Birincil Dil en
Konular Sağlık Bilimleri ve Hizmetleri
Yayımlanma Tarihi bahar
Bölüm Orjinal Araştırma
Yazarlar

Orcid: 0000-0002-5389-8243
Yazar: Fatih TURAN (Sorumlu Yazar)
Kurum: SAKARYA SAĞLIK BAKANLIĞI SAKARYA ÜNİVERSİTESİ EĞİTİM VE ARAŞTIRMA HASTANESİ
Ülke: Turkey


Orcid: 0000-0002-7204-4112
Yazar: İlyas SAYAR
Kurum: FACULTY OF MEDICINE
Ülke: Turkey


Orcid: 0000-0001-8034-1064
Yazar: İsmail SALCAN
Kurum: Erzincan Mengücekgazi Traning and Research Hospital Department of Otorhinolaryngology
Ülke: Turkey


Destekleyen Kurum Erzincan University Committee for Scientific Research Projects.
Proje Numarası SAG-A-140613-0033
Tarihler

Kabul Tarihi : 1 Mart 2020
Yayımlanma Tarihi : 31 Mart 2020

AMA TURAN F , SAYAR İ , SALCAN İ . The Application of Lipoic Acid in Experimentally Induced Tympanosclerosis in Rats. J Contemp Med. 2020; 10(1): 70-74.