Kemik defektlerinin tedavisi için β-trikalsiyum fosfat ile birleştirilmiş aljinat ve yumurta kabuğu zarı içeren biyomateryalin değerlendirilmesi

Öz

Kemik defektlerinin tedavisi için tasarlanan iskele, dondurarak kurutma yöntemi kullanılarak üretilmiştir. İskelelerin karakterizasyonu fourier transform infrared (FTIR) spektroskopisi ve X-ışını kırınım analizi, şişme ve bozunma oranı, taramalı elektron mikroskobu (SEM) testleri kullanılarak gerçekleştirilmiştir. İskelelerin biyouyumluluğunu analiz etmek için osteoblast hücreleri ile in vitro sitotoksisite deneyi yapılmıştır. Karakterizasyon sonuçları aljinat, yumurta kabuğu membranı ve β-TCP arasında güçlü iyonik etkileşimler ve birbirine bağlı gözenekli yapı olduğunu göstermiştir. Hücre canlılığının %80'den fazla olması iskelelerin toksik olmadığını göstermektedir. Tüm sonuçlar birlikte değerlendirildiğinde, aljinat/yumurta kabuğu zarı içeren β-TCP'nin kemik tedavisi uygulamaları için uygun olduğu düşünülebilir.

Anahtar Kelimeler

• aljinat
• yumurta kabuğu zarı
• beta-trikalsiyum fosfat
• doku mühendisliği

Evaluation of biomaterial containing alginate and egg-shell membrane incorporated with β-tricalcium phosphate for treatment of bone defects

Öz

The freeze-drying procedure was used to create the scaffold that was meant to cure bone deformities. Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction analysis, swelling and degradation ratio, and scanning electron microscopy (SEM) tests were used to characterize the scaffolds. Osteoblast cells were used in an in vitro cytotoxicity test to evaluate the scaffolds' biocompatibility. The results of the characterization revealed an interconnected porous structure and significant ionic connections between the alginate, egg-shell membrane, and β-tricalcium phosphate (β-TCP). The scaffolds are regarded as nontoxic because the cell viability was more than 80%. When all the findings are evaluated, β-TCP with alginate/egg-shell membrane can be regarded as appropriate for bone treatment applications.

Anahtar Kelimeler

• alginate
• egg shell membrane
• beta-tricalcium phosphate
• tissue engineering

Kaynakça

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