ANTIBACTERIAL EFFECT OF BORON-DOPPED GRAPHENE OXIDE SOLUTIONS ON STERILIZATION OF HAND TOOLS

Öz

In medicine and dentistry, sterilization is mandatory for the benefit for both patients and healthcare personnel. Especially on instruments used in surgical interventions, some microorganisms take place as a result of surgical intervention. When medical equipment is not properly disinfected or sterilized, it increases the risk of infection due to breach of host barriers. In this study, boron doped graphene oxide (BGO) sterilization pre-treatment solution was produced in order to clean dental hand instrument. Boron oxide (B2O3) and boric acid (H3BO3) and will be used as a boron source. Graphene oxide (GO) was synthesized by the Modified Improved Hummers method without using sodium nitrate (NaNO3). BGO composites was characterized using XRD, SEM/EDS, and FTIR analyses. The SEM images obtained as a result of the analyzes, the presence of characteristic peaks of GO, H3BO3 and B2O3 in the XRD analysis, and the presence of B-C and B-O bonds in the H3BO3/GO (GBA) and B2O3/GO (GBO) structures in the FTIR analysis confirmed the presence of boron in the GO structure. The antibacterial activity of BGO solution was measured for Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria by the agar well diffusion method. It has been observed that the equivalent solutions used in the industry, GBA and GBO solutions, do not have an antibacterial effect at the determined concentration. The cleaning efficiency of GBA and GBO sterilization pre-treatment solutions is similar to the equivalent product, and it has been observed that sustainable and environmentally friendly solutions in terms of raw materials can be an alternative in sterilization pre-treatment.

Anahtar Kelimeler

• Boron Oxide
• Boric Acid
• Graphene Oxide
• Sterilization
• Modified Improved Hummers

BOR KATKILI GRAFEN OKSİT ÇÖZELTİLERİN EL ALETLERİNİN STERİLİZASYONUNDA ANTİBAKTERİYEL ETKİSİ

Öz

Tıp ve diş hekimliğinde hem hasta hem sağlık personeli açısından sterilizasyon işlemi hayati derecede önem taşımaktadır. Özellikle cerrahi müdahalelerde kullanılmakta olan aletler üzerinde cerrahi müdahale sonucunda birtakım mikroorganizmalar yer alır. Tıbbi ekipman uygun şekilde dezenfekte veya sterilize edilmediğinde, konak bariyerlerinin ihlali nedeniyle enfeksiyon riskini artırmaktadır. Bu çalışmada dental el aletlerinin temizlenmesinde kullanılmak üzere bor katkılı grafen oksit (BGO) sterilizasyon ön işlem solüsyonu üretildi. Bor kaynağı olarak bor oksit (B2O3) ve borik asit (H3BO3) kullanıldı. Grafen oksit (GO) Modifiye Geliştirilmiş Hummers Metodu ile sodyum nitrat (NaNO3) kullanılmadan sentezlendi. BGO kompozitlerinin karakterizasyonu XRD, SEM/EDS ve FTIR analizleri kullanılarak yapıldı. Yapılan analizler sonucunda elde edilen SEM görüntüleri, XRD analizinde GO, H3BO3 ve B2O3’e ait karakteristik piklerin varlığı ve FTIR analizinde H3BO3/GO (GBA) ve B2O3/GO (GBO) yapılarında B-C ve B-O bağlarının görülmesi GO yapısında bor varlığını doğrulamıştır. BGO solüsyonunun antibakteriyel etkinliği agar kuyucuk difüzyon yöntemi ile Gram pozitif (Staphylococcus aureus) ve Gram negatif (Escherichia coli) bakteriler için ölçülmüştür. Belirlenen konsantrasyonda kullanılan muadil solüsyon, GBA ve GBO çözeltilerinin antibakteriyel etki göstermediği gözlenmiştir. GBA ve GBO sterilizasyon ön işlem solüsyonlarının temizlik veriminin de muadil ürünle benzer olması ham madde açısından sürdürülebilir ve çevre dostu solüsyonların sterilizasyon ön işleminde alternatif olabileceğini göstermiştir.

Anahtar Kelimeler

• Bor Oksit
• Borik Asit
• Grafen Oksit
• Sterilizasyon
• Modifiye Geliştirilmiş Hummers

Kaynakça

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