ANTİBAKTERİYEL İLAÇ SALIMI İÇİN MAGNEZYUM MİKROPARÇACIKLARININ POLİANİLİN, POLİ(LAKTİK-KO-GLİKOLİK ASİT) VE POLİ(3-AMİNOFENİLBORONİK ASİT) İLE YÜZEY MODİFİKASYONU

Öz

Magnezyum (Mg), biyouyumluluğu, düşük maliyeti ve toksik olmaması nedeniyle ilaç taşıyıcı sistemlerde dikkat çeken bir materyaldir. Bu çalışmada, Mg mikropartiküllerinin taşıyıcı özelliklerini geliştirmek amacıyla yüzeyleri polianilin (Mg/PANI), poli(laktik-ko-glikolik asit) (Mg/PLGA) ve poli(3-aminofenilboronik asit) (Mg/PAPBA) ile modifiye edilmiştir. Polimer kaplı sistemler sırasıyla siprofloksasin (Mg/PANI-CPR), amikasin (Mg/PLGA-AMK) ve streptomisin (Mg/PAPBA-STR) ile yüklenmiştir. Yapısal ve fiziko-kimyasal karakterizasyon SEM-EDS, XRD ve FTIR analizleri ile gerçekleştirilmiştir. İn vitro salım çalışmaları pH 5 ortamında kuvars kristal mikroterazi (QCM) kullanılarak yürütülmüş, antibakteriyel etkinlikler Gram-pozitif (Staphylococcus aureus, Bacillus cereus) ve Gram-negatif (Pseudomonas aeruginosa, Escherichia coli) suşlara karşı değerlendirilmiştir. Bulgular, polimer kaplamanın Mg mikropartiküllerinin yapısal bütünlüğünü koruduğunu ve etkin ilaç yüklemesi sağladığını göstermiştir. SEM-EDS sonuçları polimer ve antibiyotik varlığını doğrulamış, QCM ölçümleri ise salım kinetiğinin antibiyotiğin suda çözünürlüğüne bağlı olduğunu ortaya koymuştur. Ayrıca, ilaç yüklü tüm sistemler Gram-negatif ve Gram-pozitif bakterilere karşı belirgin bir antibakteriyel aktivite sergilemiştir. Sonuç olarak, polimer kaplı Mg mikropartikülleri kontrollü ve ayarlanabilir antibiyotik salımı için umut vadeden taşıyıcı sistemlerdir.

Anahtar Kelimeler

• Antibiyotik
• Bakteri suşu
• Magnezyum mikropartiküller
• Mikrosistemler
• Kuvars kristal mikroterazi

Destekleyen Kurum

Süleyman Demirel Üniversitesi

Proje Numarası

FDK-2019-7353

Teşekkür

Bu çalışma, Süleyman Demirel Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından FDK-2019-7353 numaralı proje kapsamında desteklenmiştir.

SURFACE MODIFICATION OF MAGNESIUM MICROPARTICLES WITH POLYANILINE, POLY(LACTIC-CO-GLYCOLIC ACID), AND POLY(3-AMINOPHENYLBORONIC ACID) FOR ANTIBACTERIAL DRUG DELIVERY

Öz

Magnesium (Mg) has attracted considerable attention as a drug carrier material owing to its biocompatibility, low cost, and non-toxic nature. In the present study, Mg microparticles were surface-modified with polyaniline (Mg/PANI), poly(lactic-co-glycolic acid) (Mg/PLGA), and poly(3-aminophenylboronic acid) (Mg/PAPBA) to enhance their carrier performance. The polymer-coated systems were subsequently loaded with ciprofloxacin (Mg/PANI-CPR), amikacin (Mg/PLGA-AMK), and streptomycin (Mg/PAPBA-STR), respectively. Structural and physicochemical characterization was carried out using scanning electron microscopy coupled with energy-dispersive spectroscopy (SEM-EDS), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). In vitro release studies were conducted at pH 5 using a quartz crystal microbalance (QCM), while antibacterial activities were evaluated against Gram-positive (Staphylococcus aureus and Bacillus cereus) and Gram-negative (Pseudomonas aeruginosa and Escherichia coli) bacterial strains. The findings demonstrated that the polymer coatings preserved the structural integrity of the Mg microparticles and enabled efficient drug loading. SEM-EDS analyses confirmed the presence of both polymeric and antibiotic-associated elements, whereas QCM measurements indicated that the release kinetics were influenced by the aqueous solubility of the loaded antibiotics. Furthermore, all drug-loaded systems exhibited pronounced antibacterial activity against both Gram-positive and Gram-negative bacteria. Overall, polymer-coated Mg microparticles represent promising and tunable platforms for controlled antibiotic delivery applications.

Anahtar Kelimeler

• Antibiotic
• Bacterial strain
• Magnesium microparticles
• Microsystems
• Quartz cyrstal microbalance

Destekleyen Kurum

Süleyman Demirel Üniversity

Proje Numarası

FDK-2019-7353

Teşekkür

This work was supported by the Süleyman Demirel University Scientific Research Projects Coordination Unit under Grant (project no: FDK-2019-7353).

Kaynakça

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