CLINOPODIUM PAMPHYLICUM SUBSP. DAVISII (CONTANDR. & QUÉZEL) GOVAERTS TOPRAK ÜSTÜ KISIMLARININ ANTİOKSİDAN, ENZİM İNHİBİSYON POTANSİYELİ VE FENOLİK PROFİLİNİN İNCELENMESİ

Öz

Amaç: Tıbbi ve aromatik bitkilerden oluşan Lamiaceae familyası Türkiye'de 46 cins ve 571 tür ile temsil edilmektedir. Calamintha Miller türlerinin geleneksel kullanımları arasında baharat olarak, uyarıcı, spazm giderici, terletici, balgam söktürücü etkileri ve bunun yanısıra mide ve boğaz ağrılarının tedavisi yer alır. C. pamphylica subsp. davisii (Contandr. & Quézel) P.H. Davis Clinopodium pamphylicum subsp. davisii (Contandr. & Quézel) Govaerts bitkisinin sinonim ismi kabul edilmiştir. C. pamphylicum subsp. davisii Türkiye'ye endemik bir türdür. Şimdiye kadar, bu bitki üzerinde uçucu yağ araştırmaları dışında herhangi bir biyolojik ve fitokimyasal araştırma bildirilmemiştir. C. pamphylicum subsp. davisii’nin antioksidan ve enzim inhibisyonu etkilerinin incelenmesi ile birlikte fitokimyasal bileşenlerinin değerlendirilmesi bu çalışmanın amaçlarıdır.

Gereç ve Yöntem: C. pamphylicum subsp. davisii’nin in vitro antioksidan potansiyelini (DPPH, ABTS, demir şelasyon, Toplam fenolik madde ve flavonoit miktarı) ve asetilkolinesteraz, butirilkolinesteraz, α-glukozidaz, α-amilaz ve tirozinaz gibi enzim inhibitör aktiviteleri spektrofotometrik yöntemler kullanılarak ölçülmüştür. Bitki ekstrelerinin fenolik bileşimini HPLC kullanarak analiz etmek için birçok fenolik asit ve flavonoitin miktarları hesaplanmıştır.

Sonuç ve Tartışma: Sonuçlarımız demir şelasyon dışındaki DPPH ve ABTS yöntemleri ile metanol ekstresinin sulu ekstreden daha yüksek antioksidan özellik sergilediğini göstermiştir. Ayrıca, metanol ekstresi, test edilen asetilkolinesteraz ve α-glukozidaz dışındaki enzimler üzerinde sulu ekstreden daha fazla inhibisyon sergilemiştir. HPLC sonuçlarına gelince, klorojenik asit methanol ekstresi için ana fenolik bileşik olarak belirlenirken, kafeik asit su ekstresinde başlıca fenolik olarak belirlenmiştir. Bu sonuçlar, Türkiye'de halk arasında geleneksel kullanımları olan C. pamphylica subsp. davisii için bilimsel bir temel oluşturmaktadır.

Anahtar Kelimeler

• Antioksidan aktivite
• clinopodium pamphylicum
• enzim inhibisyon aktivite
• fenolik bileşikler

EXAMINATION OF THE AERIAL PARTS OF CLINOPODIUM PAMPHYLICUM SUBSP. DAVISII (CONTANDR. & QUÉZEL) GOVAERTS IN TERMS OF ANTIOXIDANT AND ENZYME INHIBITION POTENTIALS, TOGETHER WITH PHENOLIC PROFILE

Öz

Objective: The family Lamiaceae, consists of medicinal and aromatic plants, is represented by 46 genera and 571 species in Turkey. Traditional uses for Calamintha Miller species include utilizing as a spice, stimulant, antispasmodic, diaphoretic, expectorant, as well as treatment for stomach and throat aches. C. pamphylica subsp. davisii (Contandr. & Quézel) P.H. Davis is accepted as synonym of Clinopodium pamphylicum subsp. davisii (Contandr. & Quézel) Govaerts. C. pamphylicum subsp. davisii is an endemic species to Turkey. Until now, no biological and phytochemical research has been reported on this plant except for its essential oil research. Examining the antioxidant, and enzyme inhibition effects of C. pamphylicum subsp. davisii, as well as determining its phytochemical composition were the targets of the current study.

Material and Method: To evaluate in vitro antioxidant potential (DPPH, ABTS, iron chelating, total phenol and flavonoid amounts) and enzyme inhibitory activities, such as acetylcholinesterase, butyrylcholinesterase, α-glucosidase, α-amylase and tyrosinase for C. pamphylicum subsp. davisii were measured with spectrophotometric methods. The quantities of different phenolic acids and flavonoids were measured using HPLC to analyze the phenolic composition of the plant extracts.

Result and Discussion: Our results indicated that except for iron chelating methanol extract exhibited higher antioxidant properties over water extract using DPPH, and ABTS methods. In addition, methanol extract displayed more inhibition on the tested enzymes except for acetylcholinesterase, and α-glucosidase than water extract. As for HPLC findings, although chlorogenic acid was signified as the main phenolic compound for the methanol extract, caffeic acid was specified as the major phenolic for the water extract. These results provide a scientific basis for C. pamphylica subsp. davisii, which possessed the traditional uses in Turkish folk medicine.

Anahtar Kelimeler

• Antioxidant activity
• clinopodium pamphylicum
• enzyme inhibition activity
• phenolic compounds

Teşekkür

Authors want to put on record their gratitude to the Professor Yavuz Bagcı, Department of Pharmaceutical Botany, Selcuk University, for providing Lab facilities. We would also like to show our gratitude to the Assoc. Prof. Hamide Filiz Ayyıldız for her technical assistant for HPLC analysis. Authors also acknowledges the financial assistance provided by Selcuk University Scientific Research Program.

Kaynakça

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