ACUTODESMUS DIMORPHUS (TURPIN) P.M. TSARENKO (SCENEDESMACEAE, CHLOROPHYTA)' NIN KİMYASAL BİLEŞİMİ VE ANTİBAKTERİYEL AKTİVİTESİNİN İN VİTRO ANALİZİ

Öz

Amaç: Yeşil mikroalgler hızlı büyüyen organizmalardır ve çeşitli biyoaktif bileşiklere ve biyomoleküllere sahip oldukları bilinmektedir. Yeşil bir mikroalg olan Acutodesmus dimorphus BIOTECH 4039'un kimyasal bileşimi ve antibakteriyel aktiviteleri incelenmiştir.

Gereç ve Yöntem: A. dimorphus'un kurutulmuş alg biyokütlesi proksimate ve elemental kompozisyon analizine tabi tutulmuştur. A. dimorphus'un metanolik ekstresi, mikroalglerin toplam fenolik içeriğini (TPC) ve antibakteriyel aktivitesini belirlemek için kullanılmıştır. TPC, Folin-Ciocalteu yöntemi kullanılarak ölçülmüştür. Öte yandan, tıbbi açıdan önemli bakteriyel patojenlere (Staphylococcus epidermidis BIOTECH 10098, penisilin asilaz üreten Bacillus cereus BIOTECH 1509, Listeria monocytogenes BIOTECH 1958) karşı antibakteriyel aktivite, Methicillin-Resistant Staphylococcus aureus BIOTECH 10378, Pseudomonas aeruginosa BIOTECH 1824, penicillin acylase-producing Escherichia coli BIOTECH 1634 ve Serratia marcescens BIOTECH 1748) modifiye Kirby-Bauer difüzyon yöntemi kullanılarak yapılmıştır.

Sonuç ve Tartışma: A. dimorphus, sırasıyla 43.19 ± 0.13, 26.92 ± 0.01 ve 14.17 ± 0.04 yüzde bileşimiyle yüksek miktarda protein, kül ve lipid içermektedir. Alg biyokütlesinin elementel besin bileşiminin azalan bir sırayla Ca > Mg > K > Mn > Na > Fe > Zn > Pb > Cu > Cd >Cr olduğu görülmüştür. Ayrıca, A. dimorphus 5.34 ± 0.09 mg GAE/g TPC değerine sahiptir. A. dimorphus ekstraktının Metisiline Dirençli Staphylococcus aureus, S. epidermidis, penisilin asilaz üreten Bacillus cereus'a karşı sırasıyla 15.1 ± 0.3 mm, 13.5 ± 0.1 mm ve 6.82 ± 0.7 mm inhibisyon zonları ile güçlü antibakteriyel aktiviteleri gözlenmiştir. Bu çalışma, A. dimorphus'un endüstriyel ve farmasötik uygulamalarda kullanılabilecek önemli bileşikler ve mikro elementler için iyi bir alternatif kaynak olarak kullanılabileceğini göstermektedir.

Anahtar Kelimeler

• Antibakteriyel aktivite
• Filipinler
• kimyasal bileşim
• mikroalg

CHEMICAL COMPOSITION AND IN VITRO ANALYSIS OF ANTIBACTERIAL ACTIVITY OF ACUTODESMUS DIMORPHUS (TURPIN) P.M. TSARENKO (SCENEDESMACEAE, CHLOROPHYTA)

Öz

Objective: Green microalgae are fast growing organisms and are known to have diverse bioactive compounds and biomolecules. The chemical composition and antibacterial activities of a green microalga, Acutodesmus dimorphus BIOTECH 4039 were studied.

Material and Method: Dried algal biomass of A. dimorphus was subjected to proximate and elemental composition analysis. Methanolic extract of A. dimorphus was used to determine the total phenolic content (TPC) and antibacterial activity of the microalga. TPC was measured using the Folin–Ciocalteu method. On the other hand, the antibacterial activity against medically important bacterial pathogens (Staphylococcus epidermidis BIOTECH 10098, penicillin acylase-producing Bacillus cereus BIOTECH 1509, Listeria monocytogenes BIOTECH 1958, Methicillin-Resistant Staphylococcus aureus BIOTECH 10378, Pseudomonas aeruginosa BIOTECH 1824, penicillin acylase-producing Escherichia coli BIOTECH 1634, and Serratia marcescens BIOTECH 1748) was done using modified Kirby-Bauer diffusion method.

Result and Discussion: A. dimorphus contained high amounts of protein, ash, and lipid with percent composition of 43.19 ± 0.13, 26.92 ± 0.01, and 14.17 ± 0.04, respectively. The elemental nutrient composition of the algal biomass was observed to be in a decreasing order of Ca > Mg > K > Mn > Na > Fe > Zn > Pb > Cu > Cd >Cr. In addition, A. dimorphus has a TPC of 5.34 ± 0.09 mg GAE/g. Potent antibacterial activities of A. dimorphus extract were observed against Methicillin-Resistant Staphylococcus aureus, S. epidermidis, penicillin acylase-producing Bacillus cereus with zones of inhibition of 15.1 ± 0.3 mm, 13.5 ± 0.1 mm, and 6.82 ± 0.7 mm, respectively. The study shows the use of A. dimorphus as good alternative source of important compounds and microelements that can be use in industrial and pharmaceutical application

Anahtar Kelimeler

• Antibacterial activity
• chemical composition
• microalgae
• Philippines
• Antibakteriyel aktivite
• Filipinler
• kimyasal bileşim
• mikroalg

Destekleyen Kurum

University of the Philippines Los Baños

Teşekkür

The author is grateful for the financial and technical support given by the PNCM-BIOTECH, UPLB and Mrs. Arsenia B. Sapin for the conduct of the research study.

Kaynakça

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