FARKLI HASTANELERDEN İZOLE EDİLEN ACİNETOBACTER BAUMANNİİ SUŞLARININ GENETİK ÇEŞİTLİLİĞİ

Yıl 2023, , 104 - 110, 20.01.2023

Hilal Basak Erol , Banu Kaşkatepe , Nurten Altanlar , Sulhiye Yıldız , Süleyman Yalçın

https://doi.org/10.33483/jfpau.1168215

Öz

Amaç: Suşların genotipik analizi, suşlar arasındaki genetik ilişkileri ve epidemiyolojik araştırmaları değerlendirmek için bilgi sağlayabilir ve farklı coğrafi bölgelerdeki dolaşımlarının izlenmesi için önemlidir. Bu çalışma, dört farklı hastaneden elde edilen klinik Acinetobacter baumannii (A. baumannii) izolatları arasındaki genetik benzerlik veya farklılıkları belirlemeyi amaçlamıştır.

Gereç ve Yöntem: Bu çalışmada, yoğun bakım ünitelerindeki hastalardan 78 A. baumannii klinik izolatı alınmıştır. Dört farklı hastaneden rastgele seçilen ve antibiyotik duyarlılığı bilinen 24 A. baumannii suşunun kolistin MIK değeri belirlenmiştir. Bu suşlar, Enterobacterial repetitive intergenic consensus (ERIC)-PCR yöntemi ile genetik olarak karakterize edilmiştir.

Sonuç ve Tartışma: Çalışmanın sonucunda, izolatların 2 kümeye (A1 ve A2) ayrıldığı ve Küme A2'nin tek bir genotip (C1) ile temsil edildiği ve birbiriyle ilişkili 23 genotipin Küme A1'de olduğunu gösterilmiştir.

Anahtar Kelimeler

Acinetobacter baumannii, direnç, ERIC-PCR, genetik çeşitlilik

Destekleyen Kurum

Ankara Üniversitesi

Proje Numarası

18L0237009

GENETIC DIVERSITY OF ACINETOBACTER BAUMANNII STRAINS ISOLATED FROM DIFFERENT HOSPITALS

Öz

Objective: The genotypic analysis of the strains can provide information to evaluate the genetic relationships among strains and epidemiological investigations, and it is crucial for monitoring their circulation in different geographic regions. This study was to aim to identify genetic similarities or dissimilarities among clinical Acinetobacter baumannii (A. baumannii) isolates from four different hospitals.

Material and Method: In this study, 78 non-duplicate clinical isolates of A. baumannii were received from patients in the critical care units. The colistin MIC values of 24 A. baumannii strains randomly selected from four different hospitals and known to have antibiotic susceptibility were determined. These strains were genetically characterized by the Enterobacterial repetitive intergenic consensus (ERIC)-PCR method.

Result and Discussion: The results of the study showed that the isolates were divided into 2 clusters (A1 and A2) and Cluster A2 was represented by a single genotype (C1) and 23 interrelated genotypes were in Cluster A1.

Anahtar Kelimeler

Acinetobacter baumannii, ERIC-PCR, genetic diversity, resistance

Proje Numarası

18L0237009

Kaynakça

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