İKİ JUNİPERUS VE PRUNUS TÜRÜNÜN BAZI BİYOLOJİK AKTİVİTELERİNİN VE KATEŞİN TANEN İÇERİKLERİNİN KARŞILAŞTIRILMASI

Öz

Amaç: J. communis var. saxatilis, J. oxycedrus subsp. oxycedrus, P. spinosa, ve P. cerasifera meyvelerinde standartlar kullanılarak LC-MS/MS ile analiz edilmiştir. Bitkilerin meyve ve kozalak ekstrelerinin antidiyabetik, antimikrobiyal ve antioksidan aktiviteleri değerlendirilmiştir. Sekonder metabolitlerin kalitatif analizi de yapılmıştır. Gereç ve Yöntem: MIC ile antimikrobiyal aktivite yapıldı. ABTS·+ ve DPPH• süpürücü testler antioksidan aktiviteler için kullanılmıştır. α-Amilaz ve α-glukosidaz inhibisyon testleri antidiyabetik aktivite için kullanılmıştır. Sonuç ve Tartışma: JCS (IC50 = 578 ± 20 μg/ml), JOO (IC50 = 3706 ± 52 μg/ml) ve PS (IC50 = 313 ± 7 μg/ml) ekstraktlarının akarbozdan (IC50 = 4061 ± 146 μg/ml) daha yüksek aktivite gösterdiği gözlemlenmiştir. Juniperus communis var. saxatilis meyve ekstresi tüm mikroorganizmalara karşı (312.5 -2500 µg/ml) arasında MİK değerleri göstermiştir. JCS en yüksek fenolik bileşime ve JOO en düşük fenolik bileşime sahiptir (µg GAE/ mg ekstrakt). JCS ekstresi en yüksek antioksidan aktiviteyi göstermiştir. En yüksek kateşin (1173.3±5.77 ng/ml) ve epigallokateşin (208±7.21 ng/ml) içeren bitki JCS’dir. Bu araştırmada en yüksek tanen içeriğine sahip JCS’nin en yüksek etkileri gösterdiği söylenebilir.

Anahtar Kelimeler

• Antidiyabetik
• antimikrobiyal
• kateşik
• Juniperus
• Prunus

COMPARISON OF SOME BIOLOGICAL ACTIVITIES AND CATECHIN TANNIN CONTENTS OF TWO JUNIPERUS AND PRUNUS SPECIES

Öz

Objective: Qualitative and quantitative determination of catechin in fruits of J. communis var. saxatilis, J. oxycedrus subsp. oxycedrus, P. spinosa, and P. cerasifera was analyzed by LC-MS/MS using standards. Antidiabetic, antimicrobial, and antioxidant activities of fruit and cone extracts of these plants were evaluated. Qualitative analysis of secondary metabolites was also done. Material and Method: Antimicrobial activity was done with MIC. ABTS·+ and DPPH• scavenging activies were used antioxidant activity. α-Amylase and α-glucosidase inhibition assays were done for antidiabetic activity. Result and Discussion: JCS (IC50 = 578 ± 20 μg/ml), JOO (IC50 = 3706 ± 52 μg/ml), and PS (IC50 = 313 ± 7 μg/ml) extracts were observed to show a higher activity than acarbose (IC50 = 4061 ± 146 μg/ml). J. communis var. saxatilis fruit extract was observed with MIC values between (312.5 -2500 µg/ml) against all microorganisms. JCS extract has the highest phenolic composition and JOO has the lowest phenolic composition (µg GAE/ mg extract). JCS extract indicated the highest antioxidant activity. It was determined that plant containing the highest catechin (1173.3±5.77 ng/ml) and epigallocatechin (208±7.21 ng/ml) was JCS. In this research, it can be said that JCS with the highest tannin content shows the highest effects.

Anahtar Kelimeler

• Antidiabetic
• antimicrobial
• catechic
• Juniperus
• Prunus

Kaynakça

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