INVESTIGATION CORRELATION BETWEEN THE ANTIOXIDANT, ANTIMICROBIAL, ANTI-FUNGI ACTIVITIES AND CONTENT OF PHENOLIC COMPOUNDS OF RASPBERRY LEAF (RUBUS IDAEUS L.) EXTRACTS

Öz

Objective: The purpose of work was study the total content of some biologically active substances (BAS), determine antimicrobial, anti-fungi and antioxidant activities of obtained raspberry leaf extracts, and study a correlation analysis between the content of natural compounds and antimicrobial/antifungal and antioxidant activities. Material and Method: The quantity of phenolic compounds, catechins, flavonoids and hydroxycinnamic acids was determined by spectrophotometric method of analysis, whereas organic acids by alkalimetric method; antioxidant activity of obtained extracts was evaluated by potentiometric method, antimicrobial and anti-fungi wad determined by method of "well". Result and Discussion: Results demonstrates the highest amount of polyphenols, flavonoids, catechins and hydroxycinnamic acids were 1.85±0.02, 1.01±0.02, 0.33±0.01 and 0.24±0.005% in 60% ethanolic extract, respectively. The organic acids were dominated in aqueous extract (1.02±0.02%). The most potent antioxidant property possessed 60% extract of raspberry leaf. There is a high correlation between the content of polyphenols, catechins and antioxidant activity, in the case of inhibition of S. aureus, P. aeruginosa, B. subtillis depends on polyphenols, catechins and antioxidant activity, whereas fungi C. albicans significantly depends only on the content of hydroxycinnamic acids as well as E. coli is not depend on any BAS. These findings show the great potential in the development and creation of new medicines with antimicrobial, antioxidant and antifungal effects that are not inferior to, and even superior to, the effects of synthetic analogues.

Anahtar Kelimeler

• Antimicrobial activity
• anti-fungi
• antioxidant activity
• correlation analysis
• polyphenols
• raspberry leaf

AHUDUDU YAPRAĞI (RUBUS IDAEUS L.) SIVI EKSTRELERİNİN ANTİOKSİDAN, ANTİMİKROBİYAL, ANTİ-FUNGAL AKTİVİTELERİ İLE FENOLİK BİLEŞİKLERİNİN İÇERİĞİ ARASINDAKİ KORELASYONUN İNCELENMESİ

Öz

Amaç: Çalışmanın amacı, bazı biyolojik olarak aktif maddelerin (BAS) toplam içeriğini incelemek, elde edilen ahududu yaprağı ekstrelerinin antimikrobiyal, anti-fungal ve antioksidan aktivitelerini belirlemek ve doğal bileşiklerin içeriği ile antimikrobiyal/antifungal ve antioksidan aktivitleri arasındaki korelasyon analizini incelemektir. Gereç ve Yöntem: Fenolik bileşikler, kateşinler, flavonoitler ve hidroksisinamik asitlerin miktarı spektrofotometrik analiz yöntemiyle, organik asitlerin miktarı ise alkalimetrik yöntemle belirlenmiştir. Elde edilen ekstrelerin antioksidan aktiviteleri potansiyometrik yöntemle değerlendirilmiş, antimikrobiyal ve anti-fungal aktivite ise "kuyu" yöntemiyle belirlenmiştir. Sonuç ve Tartışma: Sonuçlar; en yüksek polifenol, flavonoit, kateşin ve hidroksisinnamik asit miktarının sırasıyla %1.85±0.02, 1.01±0.02, 0.33±0.01 ve %0.24±0.005 olarak %60 etanolik ekstrede olduğunu göstermiştir. Sulu ekstrede organik asitler baskın bulunmuştur (%1.02±0.02). En güçlü antioksidan özelliği %60’lık ahududu yaprağı ekstresi göstermiştir. Polifenol ve kateşin içeriği ile antioksidan etki arasında yüksek korelasyon tespit edilmiştir; S. aureus, P. aeruginosa, B. subtillis'in inhibisyonunun polifenoller ile kateşinlerin içeriğine ve ve antioksidan aktiviteye bağlı olduğu görülürken; C. albicans mantarlarına karşı etkinin yalnızca hidroksisinnamik asit içeriği ile önemli ölçüde ilişkili olduğu tespit edilmiştir. E. coli üzerine etkinin ise herhangi bir BAS'a bağlı olmadığı gözlenmiştir. Bu bulgular; sentetik analogların etkilerinden daha düşük olmayan ve hatta onlardan daha üstün etkinlik göstermesi dolayısıyla, antimikrobiyal, antioksidan ve anti-fungal etkilere sahip yeni ilaçların geliştirilmesi ve yaratılmasındaki büyük potansiyeli göstermektedir.

Anahtar Kelimeler

• Ahududu yaprağı
• antibakteriyel aktivite
• anti-fungal
• antioksidan aktivite
• korelasyon analizi
• polifenoller

Kaynakça

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