DETAILED ELECTROCHEMICAL BEHAVIOR INVESTIGATION AND DETERMINATION OF ANTIPSYCHOTIC DRUG PALIPERIDONE ON A GLASSY CARBON ELECTRODE

Öz

Objective: A sensitive and cost-effective electrochemical method was developed on a bare glassy carbon electrode (GCE) for the determination of paliperidone (PAL), an antipsychotic drug used in the primary schizophrenia treatment affecting the whole world. Material and Method: The oxidation studies carried out in pH 8 phosphate buffer solution (PBS) by cyclic voltammetry (CV) showed that the oxidation reaction of PAL is a process including the same number of electrons and protons. The electrochemical detection of PAL was carried out by differential pulse voltammetry (DPV). Result and Discussion: The linearity range was between 1 µM and 100 µM. The limit of detection (LOD) and quantification (LOQ) values were calculated as 2.41x10-7 M and 8.033x10-7 M, respectively. The applicability of the developed method was confirmed by using it on synthetic human serum. The relative standard deviation (RSD) values were less than 2%. The selectivity of the analysis was demonstrated over against interfering substances such as ascorbic acid, KNO3, MgCl2, paracetamol, dopamine, Na2SO4, and uric acid with recovery% and RSD% values in the range 98.32%-101.56% and 0.15%-1.99%, respectively. The proposed method provided high sensitivity for PAL and is the first reported method for the electroanalysis of PAL.

Anahtar Kelimeler

• Differential pulse voltammetry
• electrochemistry
• glassy carbon electrode
• paliperidone

CAMSI KARBON ELEKTROT İLE ANTİPSİKOTİK İLAÇ PALİPERİDONUN AYRINTILI ELEKTROKİMYASAL DAVRANIŞININ ARAŞTIRILMASI VE TAYİNİ

Öz

Amaç: Tüm dünyayı etkileyen şizofreninin birincil tedavisinde kullanılan antipsikotik ilaç olan paliperidonun (PAL) tayini için yalın camsı karbon elektrot (CKE) kullanılarak hassas ve uygun maliyetli bir elektrokimyasal yöntem geliştirildi. Gereç ve Yöntem: pH 8 fosfat tampon çözeltisinde dönüşümlü voltametri (DV) ile gerçekleştirilen oksidasyon çalışmaları, PAL'ın oksidasyon reaksiyonunun iki elektron ve iki protonlu bir süreç olduğunu gösterdi. PAL'ın elektrokimyasal tespiti diferansiyel puls voltametrisi (DPV) ile gerçekleştirildi. Sonuç ve Tartışma: Doğrusal aralık 1x10-6 M ile 1x10-4 M arasında bulundu. Teşhis sınırı (TS) ve tayin alt sınırı (TAS) değerleri sırasıyla 2.41x10-7 M ve 8.033x10-7 M olarak hesaplandı. Geliştirilen yöntemin uygulanabilirliği sentetik insan serumu üzerinde kullanılarak doğrulandı. Bağıl standart sapma (BSS) değerleri %2'den azdı. Yöntemin seçiciliği, askorbik asit, KNO3, MgCl2, parasetamol, dopamin, Na2SO4 ve ürik asit gibi girişim etkili ajanların varlığında sırasıyla %98.32-%101.56 ve %0.15-%1.99 aralığında %geri kazanım ve %BSS değerleri ile gösterilmiştir. Önerilen yöntem PAL için yüksek hassasiyet sağlamıştır ve PAL'in elektroanalizi için bildirilen ilk yöntemdir.

Anahtar Kelimeler

• Camsı karbon elektrot
• diferansiyel darbe voltametrisi
• elektrokimya
• paliperidon

Kaynakça

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