PHENOLIC CONTENT AND ANTIBACTERIAL ACTIVITY OF COTA PESTALOZZAE BOISS. AERIAL PARTS

Öz

Objective: The genus Cota was once considered a subgenus of Anthemis but is now recognized as an independent genus. The species belonging to Cota, including several endemic to Turkey, have been less extensively studied in the literature than other plants in the Asteraceae family. The current study aimed to examine the phenolic content and antibacterial activity of C. pestalozzae Boiss., an endemic species from Turkey. Material and Method: The phenolic content of the methanol extract prepared from the aerial parts of C. pestalozzae Boiss. was analysed qualitatively and quantitatively using high-performance liquid chromatography (HPLC) analysis. This study calculated the limit of detection (LOD) and quantification (LOQ) for each detected compound. The antibacterial activity of the extract was also investigated against some Gram (+) and (-) bacteria strains. Result and Discussion: In the current study, chlorogenic acid, 4,5-O-dicaffeoyl quinic acid, 3,5-O-dicaffeoyl quinic acid, rutin, hyperoside and isoquercetin were qualitatively and quantitatively detected in the methanolic extract of the aerial parts of C. pestalozzae Boiss. Extract of the aerial parts exhibited antibacterial activity only against Staphylococcus aureus ATCC 43300 (MRSA) with a MIC value of 10000 µg/ml. The research indicates that C. pestalozzae contains phenolic compounds and is effective against Gram (+) bacteria.

Anahtar Kelimeler

• Antibacterial activity
• Asteraceae
• Cota
• flavonoids
• HPLC
• phenolic acids

COTA PESTALOZZAE BOISS TOPRAK ÜSTÜ KISIMLARININ FENOLİK İÇERİĞİ VE ANTİBAKTERİYEL AKTİVİTESİ

Öz

Amaç: Cota cinsi, Anthemis cinsinin alt türleri arasında sınıflandırılırken günümüzde ayrı bir cins olarak kabul edilmektedir. Türkiye'de de endemik türleri bulunan Cota cinsine ait türler literatürde diğer Asteracea bitkilerine kıyasla daha az çalışılmıştır. Bu çalışmada, Türkiye'de endemik bir tür olan C. pestalozzae Boiis. türünün fenolik içeriği ve antibakteriyel aktivitesinin araştırılması amaçlanmıştır. Gereç ve Yöntem: C. pestalozzae Boiss. türünün toprak üstü kısmından hazırlanan metanollü ekstrenin fenolik içeriği Yüksek Performanslı Sıvı Kromatografisi (HPLC) analizi ile kalitatif ve kantitatif olarak analiz edilmiştir. Bu çalışmada, tespit edilen bileşiklerin her biri için tespit limiti ve tayin limiti hesaplanmıştır. Ayrıca ekstrenin antibakteriyel aktivitesi bazı Gram (+) ve (-) bakteri türlerine karşı araştırılmıştır. Sonuç ve Tartışma: Yapılan çalışma, C. pestalozzae Boiss. bitkisinin toprak üstü kısmının metanollü ekstresinde klorojenik asit, 4,5-O-dikafeoil kinik asit, 3,5-O-dikafeoil kinik asit, rutin, hiperozit ve izokersetin kalitatif ve kantitatif olarak tespit edilmiştir. C. pestalozzae toprak üstü kısmı ekstresi sadece Staphylococcus aureus ATCC 43300'e (MRSA) karşı 10000 µg/ml MIC değeri ile antibakteriyel aktivite sergilemiştir. Çalışma sonuçları C. pestalozzae'nin fenolik bileşikler içerdiğini ve Gram (+) bakterilere karşı etkili olduğu göstermiştir.

Anahtar Kelimeler

• Antibakteriyel aktivite
• Asteraceae
• Cota
• fenolik asitler
• flavonoitler
• HPLC

Kaynakça

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