PRUNUS CERASUS L. YAPRAK EKSTRAKTINI KULLANARAK ALTIN NANOPARTİKÜLLERİNİN YEŞİL SENTEZİ VE KARACİĞER KANSERİ HÜCRE HATTI ÜZERİNDE ANTİPROLİFERATİF ETKİLERİNİN ARAŞTIRILMASI

Öz

Amaç: Altın nanopartiküller (AuNP'ler), ilaç dağıtımı, biyosensör ve fototermal terapi gibi nanoteknoloji uygulamalarında değerli olmalarını sağlayan benzersiz kimyasal, fiziksel ve biyolojik özelliklere sahiptir. Bu çalışma, yeşil indirgeyici ve stabilize edici ajan olarak Prunus cerasus (P. cerasus) yaprak özütü kullanılarak AuNP'leri sentezlemeyi ve yapısal ve biyolojik özelliklerini değerlendirmeyi amaçlamaktadır. Gereç ve Yöntem: AuNP'ler, çevre dostu koşullar altında P cerasus yaprak özütü kullanılarak sentezlendi. Elde edilen nanopartiküller, yapısal, morfolojik ve kararlılık özelliklerini değerlendirmek için X-ışını kırınımı (XRD), Fourier dönüşümlü kızılötesi spektroskopisi (FTIR), transmisyon elektron mikroskobu (TEM), UV-Vis spektroskopisi ve zeta potansiyel analizi kullanılarak karakterize edildi. Sonuç ve Tartışma: TEM analizi, sentezlenen AuNP'lerin ağırlıklı olarak küresel olduğunu ve ortalama boyutunun 33±13 nm olduğunu doğruladı. XRD sonuçları yüz merkezli kübik kafes yapıda olduğunu kanıtladı. Ayrıca sentezlenen AuNP'lerin yüksek stabiliteye sahip olduğu zeta potansiyel analizi ile belirlenmiştir. Biyolojik değerlendirmeler AuNP'lerin seçici sitotoksik etkiler sergilediğini, P. cerasus yaprak özütüne kıyasla karaciğer kanseri (HepG2) hücrelerine karşı yaklaşık üç kat daha yüksek antiproliferatif aktivite gösterdiğini gösterdi. Bu bulgular P. cerasus özütü aracılı AuNP'lerin kanser tedavisi için potansiyelini vurgulayarak biyomedikal nanoteknolojideki uygulamalarını desteklemektedir.

Anahtar Kelimeler

• Antiproliferatif aktivite
• AuNP
• biyosentez
• Prunus cerasus L.

GREEN SYNTHESIS OF GOLD NANOPARTICLES USING PRUNUS CERASUS L. LEAVES EXTRACT AND STUDY OF THEIR CYTOTOXIC EFFECT ON LIVER CELL LINE

Öz

Objective: Gold nanoparticles (AuNPs) possess unique chemical, physical and biological properties that make them valuable in nanotechnology applications such as drug delivery, biosensor and photothermal therapy. This study aims to synthesize AuNPs using Prunus cerasus (P. cerasus) leaves extract as a green reducing and stabilizing agent and to evaluate their structural and biological properties. Material and Method: Green synthesis of AuNPs using P. cerasus leaves extract. The obtained AuNPs were characterized using UV-Vis spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and zeta potential analysis to evaluate their structural, morphological and stability properties. Result and Discussion: TEM analysis confirmed that the synthesized AuNPs were predominantly spherical and had an average size of 33±13 nm. Furthermore, the synthesized AuNPs were determined to have high stability by zeta potential analysis. Biological evaluations showed that AuNPs exhibited selective cytotoxic effects, approximately three times higher antiproliferative activity against liver cancer (HepG2) cells compared to P. cerasus leaves extract. These findings emphasize the potential of P. cerasus extract-mediated AuNPs for cancer treatment, supporting their applications in biomedical nanotechnology.

Anahtar Kelimeler

• Antiproliferative activity
• AuNPs
• biosynthesis
• Prunus cerasus L.

Kaynakça

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