CHARACTERIZATION OF GREEN SYNTHESIZED SILVER AND ZINC NANOPARTICLES USING PROPOLIS EXTRACT AND THEIR ANTIMICROBIAL, ANTIBIOFILM, ANTIQUORUM SENSING ACTIVITIES

Öz

Objective: AgNP and ZnO NP were synthesized by green synthesis method using propolis, the characterization of nanoparticles was examined and their antimicrobial, antibiofilm and antiquarum sensing activities were examined. Material and Method: Nanoparticles were synthesized from the prepared propolis extract using 100 ml and 25 ml of extract for AgNP and ZnONP, respectively. Characterization was performed using UV-Visible (UV-VIS) spectra, XRD, EDX and SEM. Antimicrobial, antibiofilm and anti-quorum sensing activities of the nanoparticles were examined. Result and Discussion: Looking at the characterization results, AgNP and ZnO NP with very small particle sizes were synthesized. According to the antibacterial activity results, the MIC values of ZnO NPs and AgNPs were determined as 0.0625 mg/ml and 0.0250 mg/ml against S. aureus ATCC 43300 (MRSA), respectively. The percentage biofilm inhibition value of ZnO NPs and AgNPs was found to be 38.94% and 38.67%, respectively. The synthesized AgNP and ZnO NPs did not show anti-quorum sensing activity.

Anahtar Kelimeler

• Antibiofilm
• antimicrobial
• antiquorum sensing
• characterization
• green synthesis
• silver nanoparticle
• zinc nanoparticle

PROPOLİS EKSTRESİ KULLANARAK YEŞİL SENTEZLENMİŞ GÜMÜŞ VE ÇİNKO NANOPARTİKÜLLERİNİN KARAKTERİZASYONU VE ANTİMİKROBİYAL, ANTİBİYOFİLM, ÇOĞUNLUĞU ALGILAMA İNHİBİSYONU AKTİVİTELERİ

Öz

Amaç: Propolis kullanılarak yeşil sentez yöntemi ile AgNP ve ZnO NP sentezlendi, nanopartiküllerin karakterizasyonu incelendi ve antimikrobiyal, antibiyofilm ve çoğunluğu algılama inhibisyonu aktiviteleri incelendi. Gereç ve Yöntem: Hazırlanan propolis ekstresinden AgNP ve ZnONP için sırasıyla 100 ml ve 25 ml ekstre kullanılarak nanopartiküller sentezlendi. Karakterizasyon, UV-Görünür bölge (UV-VIS) spektroskopisi, XRD, EDX ve SEM kullanılarak gerçekleştirildi. Nanopartiküllerin antimikrobiyal, antibiyofilm ve çoğunluğu algılama inhibisyonu aktiviteleri incelendi. Sonuç ve Tartışma: Karakterizasyon sonuçlarına bakıldığında oldukça küçük partikül boyutuna sahip AgNP ve ZnO NP sentezlendi. Antibakteriyel aktivite sonuçlarına göre ZnO NP'leri ve AgNP'leri S. aureus ATCC 43300 (MRSA)'ya karşı sırasıyla 0.0625 mg/ml ve 0.0250 mg/ml MİK değerleri gösterdi. ZnO NP'leri ve AgNP'lerinin biyofilm inhibisyon yüzdesi değeri sırasıyla %38.94 ve %38.67 olarak bulundu. Sentezlenen AgNP ve ZnO NP çoğunluğu algılama inhibisyonu aktivitesi göstermedi.

Anahtar Kelimeler

• Antibiyofilm
• antimikrobiyal
• çinko nanopartikül
• çoğunluğu algılama inhibisyonu
• gümüş nanopartikül
• karakterizasyon
• yeşil sentez

Kaynakça

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