Araştırma Makalesi
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HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ

Yıl 2023, Cilt: 47 Sayı: 2, 349 - 359, 20.05.2023
https://doi.org/10.33483/jfpau.1128840

Öz

Amaç: Diyabet ve obezite günümüzün en yaygın sağlık problemleri arasında yer almaktadır. Bu problemlerin tedavisi için yeni ilaç kaynaklarına ihtiyaç duyulmaktadır. Toplumda geleneksel olarak kullanımı ile bilinen Hypericum scabrum L. bitkisi bu araştırmanın konusu olmuştur. Araştırma kapsamında H. scabrum bitkisinin toprak üstü kısımlarından hazırlanan etanol (%80) ekstresinin, antioksidan, antidiyabetik, anti-obezite, antikolesterol aktiviteleri ile fitokimyasal içeriğinin incelenmesi amaçlanmıştır.
Gereç ve Yöntem: H. scabrum bitkisinin toprak üstü kısımlarından hazırlanan etanol (%80) ekstresinin toplam fenol, toplam flavonoit miktar tayini yapılmış ve fitokimyasal içerik değerlendirilmiştir. Aynı zamanda ekstre içeriğinde bulunan fenolik ve flavonoit bileşenler ters faz Yüksek Performanslı Sıvı Kromatografisi YPSK ile araştırılarak miktar tayini gerçekleştirilmiştir. Ekstrenin antioksidan etkisi DPPH radikal süpürücü etki, metal bağlama kapasite, demir indirgeme gücü, ABTS radikal süpürücü etki, toplam antioksidan kapasite testleriyle değerlendirilmiştir. Ekstrenin antidiyabetik etkisinin araştırılmasında a-glukozidaz ve a-amilaz enzim inhibitör aktiviteleri incelenmiştir. Anti-obezite, lipit metobolizma üzerinde etkilerinin araştırılmasında sırasıyla pankreatik lipaz ve kolesterol esteraz enzim inhibitör aktiviteleri incelenmiştir.
Sonuç ve Tartışma: Ekstre 2 mg/ml konsantasyonda %90.16 ± 0.13 DPPH radikal süpürücü etki ve %97.53 ± 0.22 ABTS radikal süpürücü etki göstermiştir. Ekstre 2 mg/ml konsantrasyonda a-glukozidaz enzimi üzerinde %98.80 ± 0.51 inhibitör etki göstermiş olup, bu değer standart madde olarak kullanılan akarboza (%99.13 ± 0.11) yakın bulunmuştur. Ekstre 2 mg/ml konsantrasyonda kolesterol esteraz enzimi üzerinde %68.63 ± 1.05 inhibitör etki göstermiştir. Ters faz YPSK analiz sonucuna göre ekstrenin 1.047 ± 0.000 g/100 g ekstre klorojenik asit içerdiği bulunmuştur. Bu bulgulara göre ekstrenin antidiyabetik etkili doğal bir kaynak olabileceği ve bu etkinin incelenmesi için daha ileri çalışmalara ihtiyaç duyulduğu sonucuna varılmıştır.

Kaynakça

  • 1. Boles, A., Kandimalla, R., Reddy, P.H. (2017). Dynamics of diabetes and obesity: Epidemiological perspective. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1863(5), 1026-1036. [CrossRef]
  • 2. Al-Goblan, A.S., Al-Alfi, M.A., Khan, M.Z. (2014). Mechanism linking diabetes mellitus and obesity. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 7, 587-591. [CrossRef]
  • 3. Simonen, P.P., Gylling, H.K., Miettinen, T.A. (2002). Diabetes contributes to cholesterol metabolism regardless of obesity. Diabetes Care, 25(9), 1511-1515. [CrossRef]
  • 4. Rahimi, R., Nikfar, S., Larijani, B., Abdollahi, M. (2005). A review on the role of antioxidants in the management of diabetes and its complications. Biomedicine Pharmacotherapy, 59(7), 365-373. [CrossRef]
  • 5. Sharopov, F.S., Gulmurodov, I.S., Setzer, W.N. (2010). Essential oil composition of Hypericum perforatum L. and Hypericum scabrum L. growing wild in Tajikistan. Journal of Chemical and Pharmaceutical Research, 2(6), 284-290.
  • 6. Jiang, L., Numonov, S., Bobakulov, K., Qureshi, M.N., Zhao, H., Aisa, H.A. (2015). Phytochemical profiling and evaluation of pharmacological activities of Hypericum scabrum L. Molecules, 20(6), 11257-11271. [CrossRef]
  • 7. Eslami, B., Nabavi, S.F., Nabavi, S.M., Ebrahimzadeh, M.A., Mahmoudi, M. (2011). Pharmacological activities of Hypericum scabrum L. European Review for Medical and Pharmacological Sciences, 15(5), 32-37.
  • 8. Baytop T (1999): Therapy with Medicinal Plants in Turkey; Today and in Future. Istanbul, Istanbul University Press, pp. 166– 167.
  • 9. Davis, P.H. (1970). Flora of Turkey and the East Aegean Islands. Vol. 3. Flora of Turkey and the East Aegean Islands. Vol. 3.
  • 10. Tatli A (1988): Important Range Plants of Erzurum Province. Ankara, Turkey, Food and Agricultural Organization of the United Nations Press, pp. 1–77
  • 11. Pirbalouti, A.G., Rahnama, G.H., Malekpoor, F., Broujeni, H.R. (2011). Variation in antibacterial activity and phenolic content of Hypericum scabrum L. populations. Journal of Medicinal Plants Research, 5(17), 4119-4125.
  • 12. Özen, H.Ç., Başhan, M. (2003). The composition of fatty acids in Hypericum scabrum, H. scabroides and H. amblysepalum. Turkish Journal of Chemistry, 27(6), 723-726.
  • 13. Matsuhisa, M., Shikishima, Y., Takaishi, Y., Honda, G., Ito, M., Takeda, Y., Shibata, H., Higuti, T., Kodzhimatov, O.K., Ashurmetov, O. (2002). Benzoylphloroglucinol Derivatives from Hypericum scabrum. Journal of Natural Products, 65(3), 290-294. [CrossRef]
  • 14. Ayan, A.K., Radušienė, J., Çirak, C., Ivanauskas, L., Janulis, V. (2009). Secondary metabolites of Hypericum scabrum and Hypericum bupleuroides. Pharmaceutical Biology, 47(9), 847-853. [CrossRef]
  • 15. Tanker, N. (1971). Studies on Hypericum scabrum L. Journal of Faculty of Pharmacy of Ankara University, 1(1), 10-15.
  • 16. Dalar, A. (2018). Plant taxa used in the treatment of diabetes in Van Province, Turkey. International Journal of Secondary Metabolite, 5(3), 171-185. [CrossRef]
  • 17. Edirs, S., Jiang, L., Xin, X., Aisa, H.A. (2018). Anti-diabetic effect and mechanism of Kursi Wufarikun Ziyabit in L6 rat skeletal muscle cells. Journal of Pharmacological Sciences, 137(2), 212-219. [CrossRef]
  • 18. Barış, D., Kızıl, M., Aytekin, Ç., Kızıl, G., Yavuz, M., Çeken, B., Ertekin, A. S. (2011). In vitro antimicrobial and antioxidant activity of ethanol extract of three Hypericum and three Achillea species from Turkey. International Journal of Food Properties, 14(2), 339-355. [CrossRef]
  • 19. Zongo, C., Savadogo, A., Ouattara, L., Bassole, I. H.N., Ouattara, C.A.T., Ouattara, A.S., Barro, N., Koudou, J., Traore, A.S. (2010). Polyphenols content, antioxidant and antimicrobial activities of Ampelocissus grantii (Baker) Planch. (Vitaceae): a medicinal plant from Burkina Faso. International Journal of Pharmacology, 6(6), 880-887. [CrossRef]
  • 20. Kosalec, I., Bakmaz, M., Pepeljnjak, S., Vladimir-Knezevic, S. (2004). Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharmaceutica, 54(1), 65-72.
  • 21. Jung, H.A., Jin, S.E., Choi, R.J., Manh, H.T., Kim, Y.S., Min, B.S., Son, Y.K, Ahn, B.R., Kim, B.W. Sohn, H.S., Choi, J.S. (2011). Anti-tumorigenic activity of sophoflavescenol against Lewis lung carcinoma in vitro and in vivo. Archives of Pharmacal Research, 34(12), 2087-2099. [CrossRef]
  • 22. Dinis, T.C., Madeira, V.M., Almeida, L.M. (1994). Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1), 161-169. [CrossRef]
  • 23. Orhan, N., Orhan, D.D., Gökbulut, A., Aslan, M., Ergun, F. (2017). Comparative analysis of chemical profile, antioxidant, in-vitro and in-vivo antidiabetic activities of Juniperus foetidissima Willd. and Juniperus sabina L. Iranian Journal of Pharmaceutical Research: IJPR, 16(Suppl), 64-74.
  • 24. Prieto, P., Pineda, M., Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 269(2), 337-341. [CrossRef]
  • 25. Lam, S.H., Chen, J.M., Kang, C.J., Chen, C.H., Lee, S.S. (2008). α-Glucosidase inhibitors from the seeds of Syagrus romanzoffiana. Phytochemistry, 69(5), 1173-1178. [CrossRef]
  • 26. Ali, H., Houghton, P.J., Soumyanath, A. (2006). α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology, 107(3), 449-455. [CrossRef]
  • 27. Lee, Y.M., Kim, Y.S., Lee, Y., Kim, J., Sun, H., Kim, J.H., Kim, J.S. (2012). Inhibitory activities of pancreatic lipase and phosphodiesterase from Korean medicinal plant extracts. Phytotherapy Research, 26(5), 778-782. [CrossRef]
  • 28. Ngamukote, S., Mäkynen, K., Thilawech, T., Adisakwattana, S. (2011). Cholesterol-lowering activity of the major polyphenols in grape seed. Molecules, 16(6), 5054-5061. [CrossRef]
  • 29. Seyrekoglu, F., Temiz, H., Eser, F., Yildirim, C. (2022). Comparison of the antioxidant activities and major constituents of three Hypericum species (H. perforatum, H. scabrum and H. origanifolium) from Turkey. South African Journal of Botany, 146, 723-727. [CrossRef]
  • 30. Ayan, A.K., Çırak, C., Güney, K. (2008). Seasonal variation of hypericin and pseudohypericin contents in Hypericum scabrum L. growing wild in Turkey. Natural Product Communications, 3(2), 241-244. [CrossRef]
  • 31. Zarei, M.A., Tahazadeh, H. (2019). Alpha-glucosidase inhibitory activity in methanol extract of some plants from Kurdistan province. Journal of Medicinal Plants, 18(72), 227-235. [CrossRef]
  • 32. Llorent-Martínez, E.J., Zengin, G., Lobine, D., Molina-García, L., Mollica, A., Mahomoodally, M.F. (2018). Phytochemical characterization, in vitro and in silico approaches for three Hypericum species. New Journal of Chemistry, 42(7), 5204-5214. [CrossRef]
  • 33. Faraji, N., Ganji, A., Heshami, N., Salehi, I., Haddadian, A., Shojaie, S., Komaki, A. (2021). Hypolipidemic effects of Hypericum scabrum extract on the serum lipid profile and obesity in high-fat diet fed rats. Human Antibodies, 29(1), 55-61. [CrossRef]

EVALUATION OF ANTIOXIDANT, ENZYME INHIBITOR ACTIVITY OF HYPERICUM SCABRUM EXTRACT AND HPLC ANALYSIS

Yıl 2023, Cilt: 47 Sayı: 2, 349 - 359, 20.05.2023
https://doi.org/10.33483/jfpau.1128840

Öz

Objective: Diabetes and obesity are among the most common health problems today. New drug sources are needed to treat these problems. Hypericum scabrum L. , which is known for its traditional use among the public, has been the subject of this research. Within the scope of the research, it was aimed to investigate the antioxidant, antidiabetic, anti-obesity, anticholesterol activities, and phytochemical content of ethanol (80%) extract prepared from the aerial parts of the H. scabrum.
Material and Method: The total phenol, total flavonoid contents, and phytochemical content of the ethanol (80%) extract prepared from the aerial parts of the H. scabrum were evaluated. Additionally, the phenolic and flavonoid compounds in the extract were investigated with reverse phase HPLC and their quantitative analysis was carried out. The antioxidant effect of the extract was evaluated with DPPH radical-scavenging effect, metal chelating capacity, ferric reducing power, ABTS radical scavenging effect, and total antioxidant capacity tests. In the investigation of the antidiabetic effect of the extract, α-glucosidase and α-amylase enzyme inhibitory activities were investigated. To investigate the effects of anti-obesity and lipid metabolism, pancreatic lipase enzyme inhibitory activity and cholesterol esterase enzyme inhibitory activity were investigated, respectively.
Result and Discussion: The extract showed 90.16 ± 0.13% DPPH radical scavenging and 97.53 ± 0.22% ABTS radical scavenging activity at 2 mg/ml concentration. The extract had an inhibitory activity of 98.80 ± 0.51 % on the α-glucosidase enzyme at a concentration of 2 mg/ml, and this value was close to the acarbose result used as the standard substance (99.13 ± 0.11%). The extract showed inhibitory activity of 68.63 ± 1.05% on the cholesterol esterase enzyme at a concentration of 2 mg/ml. According to the results of reversed-phase HPLC analysis, it was found that the extract contained 1.047 ± 0.000 g/100 g extract chlorogenic acid. According to the results, it was concluded that the extract may be a natural source with antidiabetic effects and further studies are needed to investigate this effect.

Kaynakça

  • 1. Boles, A., Kandimalla, R., Reddy, P.H. (2017). Dynamics of diabetes and obesity: Epidemiological perspective. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1863(5), 1026-1036. [CrossRef]
  • 2. Al-Goblan, A.S., Al-Alfi, M.A., Khan, M.Z. (2014). Mechanism linking diabetes mellitus and obesity. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 7, 587-591. [CrossRef]
  • 3. Simonen, P.P., Gylling, H.K., Miettinen, T.A. (2002). Diabetes contributes to cholesterol metabolism regardless of obesity. Diabetes Care, 25(9), 1511-1515. [CrossRef]
  • 4. Rahimi, R., Nikfar, S., Larijani, B., Abdollahi, M. (2005). A review on the role of antioxidants in the management of diabetes and its complications. Biomedicine Pharmacotherapy, 59(7), 365-373. [CrossRef]
  • 5. Sharopov, F.S., Gulmurodov, I.S., Setzer, W.N. (2010). Essential oil composition of Hypericum perforatum L. and Hypericum scabrum L. growing wild in Tajikistan. Journal of Chemical and Pharmaceutical Research, 2(6), 284-290.
  • 6. Jiang, L., Numonov, S., Bobakulov, K., Qureshi, M.N., Zhao, H., Aisa, H.A. (2015). Phytochemical profiling and evaluation of pharmacological activities of Hypericum scabrum L. Molecules, 20(6), 11257-11271. [CrossRef]
  • 7. Eslami, B., Nabavi, S.F., Nabavi, S.M., Ebrahimzadeh, M.A., Mahmoudi, M. (2011). Pharmacological activities of Hypericum scabrum L. European Review for Medical and Pharmacological Sciences, 15(5), 32-37.
  • 8. Baytop T (1999): Therapy with Medicinal Plants in Turkey; Today and in Future. Istanbul, Istanbul University Press, pp. 166– 167.
  • 9. Davis, P.H. (1970). Flora of Turkey and the East Aegean Islands. Vol. 3. Flora of Turkey and the East Aegean Islands. Vol. 3.
  • 10. Tatli A (1988): Important Range Plants of Erzurum Province. Ankara, Turkey, Food and Agricultural Organization of the United Nations Press, pp. 1–77
  • 11. Pirbalouti, A.G., Rahnama, G.H., Malekpoor, F., Broujeni, H.R. (2011). Variation in antibacterial activity and phenolic content of Hypericum scabrum L. populations. Journal of Medicinal Plants Research, 5(17), 4119-4125.
  • 12. Özen, H.Ç., Başhan, M. (2003). The composition of fatty acids in Hypericum scabrum, H. scabroides and H. amblysepalum. Turkish Journal of Chemistry, 27(6), 723-726.
  • 13. Matsuhisa, M., Shikishima, Y., Takaishi, Y., Honda, G., Ito, M., Takeda, Y., Shibata, H., Higuti, T., Kodzhimatov, O.K., Ashurmetov, O. (2002). Benzoylphloroglucinol Derivatives from Hypericum scabrum. Journal of Natural Products, 65(3), 290-294. [CrossRef]
  • 14. Ayan, A.K., Radušienė, J., Çirak, C., Ivanauskas, L., Janulis, V. (2009). Secondary metabolites of Hypericum scabrum and Hypericum bupleuroides. Pharmaceutical Biology, 47(9), 847-853. [CrossRef]
  • 15. Tanker, N. (1971). Studies on Hypericum scabrum L. Journal of Faculty of Pharmacy of Ankara University, 1(1), 10-15.
  • 16. Dalar, A. (2018). Plant taxa used in the treatment of diabetes in Van Province, Turkey. International Journal of Secondary Metabolite, 5(3), 171-185. [CrossRef]
  • 17. Edirs, S., Jiang, L., Xin, X., Aisa, H.A. (2018). Anti-diabetic effect and mechanism of Kursi Wufarikun Ziyabit in L6 rat skeletal muscle cells. Journal of Pharmacological Sciences, 137(2), 212-219. [CrossRef]
  • 18. Barış, D., Kızıl, M., Aytekin, Ç., Kızıl, G., Yavuz, M., Çeken, B., Ertekin, A. S. (2011). In vitro antimicrobial and antioxidant activity of ethanol extract of three Hypericum and three Achillea species from Turkey. International Journal of Food Properties, 14(2), 339-355. [CrossRef]
  • 19. Zongo, C., Savadogo, A., Ouattara, L., Bassole, I. H.N., Ouattara, C.A.T., Ouattara, A.S., Barro, N., Koudou, J., Traore, A.S. (2010). Polyphenols content, antioxidant and antimicrobial activities of Ampelocissus grantii (Baker) Planch. (Vitaceae): a medicinal plant from Burkina Faso. International Journal of Pharmacology, 6(6), 880-887. [CrossRef]
  • 20. Kosalec, I., Bakmaz, M., Pepeljnjak, S., Vladimir-Knezevic, S. (2004). Quantitative analysis of the flavonoids in raw propolis from northern Croatia. Acta Pharmaceutica, 54(1), 65-72.
  • 21. Jung, H.A., Jin, S.E., Choi, R.J., Manh, H.T., Kim, Y.S., Min, B.S., Son, Y.K, Ahn, B.R., Kim, B.W. Sohn, H.S., Choi, J.S. (2011). Anti-tumorigenic activity of sophoflavescenol against Lewis lung carcinoma in vitro and in vivo. Archives of Pharmacal Research, 34(12), 2087-2099. [CrossRef]
  • 22. Dinis, T.C., Madeira, V.M., Almeida, L.M. (1994). Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315(1), 161-169. [CrossRef]
  • 23. Orhan, N., Orhan, D.D., Gökbulut, A., Aslan, M., Ergun, F. (2017). Comparative analysis of chemical profile, antioxidant, in-vitro and in-vivo antidiabetic activities of Juniperus foetidissima Willd. and Juniperus sabina L. Iranian Journal of Pharmaceutical Research: IJPR, 16(Suppl), 64-74.
  • 24. Prieto, P., Pineda, M., Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 269(2), 337-341. [CrossRef]
  • 25. Lam, S.H., Chen, J.M., Kang, C.J., Chen, C.H., Lee, S.S. (2008). α-Glucosidase inhibitors from the seeds of Syagrus romanzoffiana. Phytochemistry, 69(5), 1173-1178. [CrossRef]
  • 26. Ali, H., Houghton, P.J., Soumyanath, A. (2006). α-Amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to Phyllanthus amarus. Journal of Ethnopharmacology, 107(3), 449-455. [CrossRef]
  • 27. Lee, Y.M., Kim, Y.S., Lee, Y., Kim, J., Sun, H., Kim, J.H., Kim, J.S. (2012). Inhibitory activities of pancreatic lipase and phosphodiesterase from Korean medicinal plant extracts. Phytotherapy Research, 26(5), 778-782. [CrossRef]
  • 28. Ngamukote, S., Mäkynen, K., Thilawech, T., Adisakwattana, S. (2011). Cholesterol-lowering activity of the major polyphenols in grape seed. Molecules, 16(6), 5054-5061. [CrossRef]
  • 29. Seyrekoglu, F., Temiz, H., Eser, F., Yildirim, C. (2022). Comparison of the antioxidant activities and major constituents of three Hypericum species (H. perforatum, H. scabrum and H. origanifolium) from Turkey. South African Journal of Botany, 146, 723-727. [CrossRef]
  • 30. Ayan, A.K., Çırak, C., Güney, K. (2008). Seasonal variation of hypericin and pseudohypericin contents in Hypericum scabrum L. growing wild in Turkey. Natural Product Communications, 3(2), 241-244. [CrossRef]
  • 31. Zarei, M.A., Tahazadeh, H. (2019). Alpha-glucosidase inhibitory activity in methanol extract of some plants from Kurdistan province. Journal of Medicinal Plants, 18(72), 227-235. [CrossRef]
  • 32. Llorent-Martínez, E.J., Zengin, G., Lobine, D., Molina-García, L., Mollica, A., Mahomoodally, M.F. (2018). Phytochemical characterization, in vitro and in silico approaches for three Hypericum species. New Journal of Chemistry, 42(7), 5204-5214. [CrossRef]
  • 33. Faraji, N., Ganji, A., Heshami, N., Salehi, I., Haddadian, A., Shojaie, S., Komaki, A. (2021). Hypolipidemic effects of Hypericum scabrum extract on the serum lipid profile and obesity in high-fat diet fed rats. Human Antibodies, 29(1), 55-61. [CrossRef]
Toplam 33 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Eczacılık ve İlaç Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Hasya Nazlı Gök 0000-0003-4115-7509

Semih Bulut 0000-0002-4098-0221

Didem Deliorman Orhan 0000-0003-3916-4048

Erken Görünüm Tarihi 17 Mayıs 2023
Yayımlanma Tarihi 20 Mayıs 2023
Gönderilme Tarihi 15 Haziran 2022
Kabul Tarihi 12 Ocak 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 47 Sayı: 2

Kaynak Göster

APA Gök, H. N., Bulut, S., & Deliorman Orhan, D. (2023). HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ. Journal of Faculty of Pharmacy of Ankara University, 47(2), 349-359. https://doi.org/10.33483/jfpau.1128840
AMA Gök HN, Bulut S, Deliorman Orhan D. HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ. Ankara Ecz. Fak. Derg. Mayıs 2023;47(2):349-359. doi:10.33483/jfpau.1128840
Chicago Gök, Hasya Nazlı, Semih Bulut, ve Didem Deliorman Orhan. “HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ”. Journal of Faculty of Pharmacy of Ankara University 47, sy. 2 (Mayıs 2023): 349-59. https://doi.org/10.33483/jfpau.1128840.
EndNote Gök HN, Bulut S, Deliorman Orhan D (01 Mayıs 2023) HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ. Journal of Faculty of Pharmacy of Ankara University 47 2 349–359.
IEEE H. N. Gök, S. Bulut, ve D. Deliorman Orhan, “HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ”, Ankara Ecz. Fak. Derg., c. 47, sy. 2, ss. 349–359, 2023, doi: 10.33483/jfpau.1128840.
ISNAD Gök, Hasya Nazlı vd. “HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ”. Journal of Faculty of Pharmacy of Ankara University 47/2 (Mayıs 2023), 349-359. https://doi.org/10.33483/jfpau.1128840.
JAMA Gök HN, Bulut S, Deliorman Orhan D. HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ. Ankara Ecz. Fak. Derg. 2023;47:349–359.
MLA Gök, Hasya Nazlı vd. “HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ”. Journal of Faculty of Pharmacy of Ankara University, c. 47, sy. 2, 2023, ss. 349-5, doi:10.33483/jfpau.1128840.
Vancouver Gök HN, Bulut S, Deliorman Orhan D. HYPERICUM SCABRUM L. EKSTRESİNİN ANTİOKSİDAN, ENZİM İNHİBİTÖR AKTİVİTESİNİN DEĞERLENDİRİLMESİ VE YPSK ANALİZİ. Ankara Ecz. Fak. Derg. 2023;47(2):349-5.

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.