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INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS

Yıl 2024, Cilt: 48 Sayı: 1, 46 - 55, 20.01.2024
https://doi.org/10.33483/jfpau.1336857

Öz

Objective: This study aims to investigate the anticancer potential of Prangos Heyniae H. Duman & M. F. Watson root extracts against human hepatoma cells, and examine the molecular mechanisms potentially involved in extract-induced cytotoxicity.
Material and Method: HepG2 cells were treated with chloroform, n-hexane, or methanol extracts from roots of P. heyniae to investigate the possible effects on cell viability. Following the determination of IC50 values by the MTT test, n-hexane, and methanol extracts were excluded because of their selectivity indices. The chemical characterization of chloroform extract was performed by HPLC to understand the chemical composition-bioactivity relationship. Alterations induced by chloroform extract on mitochondrial membrane potential and caspase-3 activation were further investigated. In addition, cell viability was measured in the presence of different selective inhibitors of pathways to define the type of cell death pathway contributing to cytotoxicity.
Result and Discussion: Chloroform extract but not n-hexane or methanol extracts led to strong and selective inhibition of cell viability on HepG2 cells. In addition, cytotoxicity increased by chloroform extract was only restored in the presence of a pan-caspase apoptosis inhibitor. Also, treatment of HepG2 cells with chloroform extract impaired mitochondrial membrane potential and led to significant caspase-3 activation. Oxypeucedanin, isoimperatorin, and osthole were detected as the major components of the chloroform extract. These results represent that apoptosis may be involved in the anticancer effect of coumarin and furanocoumarin derivatives in chloroform extract.

Kaynakça

  • 1. Yang, J.D., Roberts, L.R. (2010). Epidemiology and management of hepatocellular carcinoma. Infectious Disease Clinics of North America, 24(4), 899-919. [CrossRef]
  • 2. Bousbaa, H. (2021). Novel anticancer strategies. Pharmaceutics, 13(2), 275. [CrossRef]
  • 3. Greenwell, M., Rahman, P.K. (2015). Medicinal plants: Their use in anticancer treatment. International Journal of Pharmaceutical Sciences and Research, 6(10), 4103-4112. [CrossRef]
  • 4. Newman, D.J., Cragg, G.M. (2012). Natural products as sources of new drugs over the 30 years from 1981 to 2010. Journal of Natural Products, 75(3), 311-335. [CrossRef]
  • 5. Lyskov, D.F., Degtjareva, G.V., Samigullin, T.H., Pimenov, M.G. (2017). The revision of Prangos subsections Koelzella and Fedtschenkoana (Apiaceae) with some notes to phylogeny and biogeography of the genus: Molecular and morphological evidence. Plant Systematics and Evolution, 303(7), 815-826. [CrossRef]
  • 6. Menemen, Y. (2012). Türkiye bitkileri listesi:(damarlı bitkiler) (1st ed.; A. Guner, ed.). İstanbul: Nezahat Gökyiğit Botanik Bahçesi Yayınları.
  • 7. Mottaghipisheh, J., Kiss, T., Tóth, B., Csupor, D. (2020). The Prangos genus: A comprehensive review on traditional use, phytochemistry, and pharmacological activities. Phytochemistry Reviews, 19, 1449-1470. [CrossRef]
  • 8. Farkhad, N.K., Farokhi, F., Tukmacki, A. (2012). Hydro-alcoholic extract of the root of Prangos ferulacea (L.) Lindl can improve serum glucose and lipids in alloxan-induced diabetic rats. Avicenna Journal of Phytomedicine, 2(4), 179.
  • 9. Albayrak, G., Demir, S., Koyu H., Baykan, S. (2022). Anticholinesterase and antityrosinase activities of endemic Prangos heyniae H. Duman and M.F. Watson and its metabolites. İstanbul Journal of Pharmacy, 53(1), 51-57. [CrossRef]
  • 10. Shokoohinia, Y., Sajjadi, S.E., Gholamzadeh, S., Fattahi, A., Behbahani, M. (2014). Antiviral and cytotoxic evaluation of coumarins from Prangos ferulacea. Pharmaceutical Biology, 52(12), 1543-1549. [CrossRef]
  • 11. Loizzo, M.R., Tundis, R., Menichini, F. (2008) Antiproliferative effects of essential oils and their major constituents inhuman renal adenocarcinoma and amelanotic melanoma cells. Cell Proliferation, 41, 1002-1012. [CrossRef]
  • 12. Razavi, S.M., Zarrini, G., Zahri, S., Mohammadi, S. (2010). Biological activity of Prangos uloptera DC. roots, a medicinal plant from Iran. Natural Product Research, 24(9), 797-803. [CrossRef]
  • 13. Yazici-Tutunis, S., Erucar, F.M., Oztas, E., Akalin, E., Ozhan, G., Miski, M., Tan, N. (2021). Chemical composition and cytotoxic effect of Prangos turcica. Records of Natural Products, 15(6), 503-512, [CrossRef]
  • 14. Sarghaleh, S.J., Behbahani, B.A., Hojjati, M.H. (2023). Evaluation of the constituent compounds, antioxidant, anticancer, and antimicrobial potential of Prangos ferulacea plant extract and its effect on Listeria monocytogenes virulence gene expression. Frontiers in Microbiology, 14, 1202228. [CrossRef]
  • 15. Ahmed, J., Güvenç, A., Küçükboyacı, N., Baldemir, A., Coşkun, M. (2011). Total phenolic contents and antioxidant activities of Prangos Lindl. (Umbelliferae) species growing in Konya province (Turkey) Turkish Journal of Biology, 35(3), 353-360 [CrossRef]
  • 16. Albayrak, G., Demir, S., Kose, F.A., Baykan, S. (2023). New coumarin glycosides from endemic Prangos heyniae H. Duman and M.F. Watson. Natural Product Research, 37(2), 227-239. [CrossRef]
  • 17. Tan, X. W., Xia, H., Xu, J.H., Cao, J.G. (2009). Induction of apoptosis in human liver carcinoma HepG2 cell line by 5-allyl-7-gen-difluoromethylenechrysin. World Journal of Gastroenterology, 15(18), 2234-2239. [CrossRef]
  • 18. Sevin, G., Alan, E., Demir, S., Albayrak, G., Demiroz, T., Yetik-Anacak, G., Baykan, S. (2022). Comparative evaluation of relaxant effects of three prangos species on mouse corpus cavernosum: Chemical characterization and the relaxant mechanisms of action of P. pabularia and (+)-oxypeucedanin. Journal of Ethnopharmacology, 284, 114823. [CrossRef]
  • 19. Sazonova, E.V., Chesnokov, M.S., Zhivotovsky, B., Kopeina, G.S. (2022). Drug toxicity assessment: Cell proliferation versus cell death. Cell Death Discovery, 8(1), 417. [CrossRef]
  • 20. Chalmers, S., McCarron, J.G. (2008). The mitochondrial membrane potential and Ca2+ oscillations in smooth muscle. Journal of Cell Science, 121, 75-85. [CrossRef]
  • 21. Ghosh, R., Goswami, S.K., Feitoza, L.F., Hammock, B., Gomes, A.V. (2016). Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts. International Journal of Cardiology, 223, 923-935. [CrossRef]
  • 22. Bashir, R., Ahmad Zargar, O., Hamid Dar, A., Yedukondalu, N., Parvaiz, Q., Hamid, R. (2022). The modulation of PI3K/Akt pathway by 3β hydroxylup-12-en-28-oic acid isolated from Thymus linearis induces cell death in HCT-116 cells. Chemical Biology and Drug Design, 99(1), 162-178. [CrossRef]
  • 23. Lee, Y.T., Wang, J.J., Luu, M., Noureddin, M., Kosari, K., Agopian, V.G., Rich, N.E., Lu, S.C., Tseng, H.R., Nissen, N.N., Singal, A.G., Yang, J.D. (2021). The Mortality and overall survival trends of primary liver cancer in the United States. Journal of the National Cancer Institute, 113(11), 1531-1541. [CrossRef]
  • 24. Yarchoan, M., Agarwal, P., Villanueva, A., Rao, S., Dawson, L.A., Lovet, J.M., Finn, R.S., Groopman, J.D., El-Serag, H.B., Monga, S.P., Wang, X.W., Karin, M., Schwartz, R.E., Tanabe, K.K., Roberts, L.R., Gunaratne, P.H., Tsung, A., Brown, K.A., Lawrence, T.S., Salem, R., Ahmed, M.M. (2019). Recent developments and therapeutic strategies against hepatocellular carcinoma. Cancer Research, 79(17), 4326-4330. [CrossRef]
  • 25. Singh, S., Sharma, B., Kanwar, S.S., Kumar, A. (2016). Lead phytochemicals for anticancer drug development. Frontiers in Plant Science. 7, 8973. [CrossRef]
  • 26. Kissin, I. (2013). An early indicator of drug success: Top journal selectivity index. Drug Design, Development, and Therapy, 7, 93-98. [CrossRef]
  • 27. Elmore, S. (2007). Apoptosis: A review of programmed cell death. Toxicologic Pathology, 35(4), 495-516. [CrossRef]
  • 28. Wang, C., Youle, R.J. (2009). The role of mitochondria in apoptosis. Annual Review of Genetics, 43, 95-118. [CrossRef]
  • 29. Park, W., Park, S., Song, G., Lim, W. (2019). Inhibitory effects of osthole on human breast cancer cell progression via induction of cell cycle arrest, mitochondrial dysfunction, and ER stress. Nutrients, 11(11), 2777. [CrossRef]
  • 30. Liang, J., Zhou, J., Xu, Y., Huang, X., Wang, X., Huang, W., Li, H. (2020). Osthole inhibits ovarian carcinoma cells through LC3-mediated autophagy and GSDME-dependent pyroptosis except for apoptosis. European Journal of Pharmacology, 874, 172990. [CrossRef]
  • 31. Bae, H., Lee, J.Y., Song, J., Song, G., Lim, W. (2021). Osthole interacts with an ER-mitochondria axis and facilitates tumor suppression in ovarian cancer. Journal of Cellular Physiology, 236(2), 1025-1042. [CrossRef]
  • 32. Choudhary, G.S., Al-Harbi, S., Almasan, A. (2015). Caspase-3 activation is a critical determinant of genotoxic stress-induced apoptosis. Methods in Molecular Biology, 1219, 1-9. [CrossRef]
  • 33. Kang, T.J., Lee, S.Y., Singh, R.P., Agarwal, R., Yim, D.S. (2009). Anti-tumor activity of oxypeucedanin from Ostericum koreanum against human prostate carcinoma DU145 cells. Acta Oncologica, 48(6), 895-900. [CrossRef]
  • 34. Ye, J., Sun, D., Yu, Y., Yu, J. (2020). Osthole resensitizes CD133+ hepatocellular carcinoma cells to cisplatin treatment via PTEN/AKT pathway. Aging, 12(14), 14406-14417. [CrossRef]
  • 35. Shokoohinia, Y., Hosseinzadeh, L., Alipour, M., Mostafaie, A., Mohammadi-Motlagh, H.R. (2014). Comparative evaluation of cytotoxic and apoptogenic effects of several coumarins on human cancer cell lines: osthole induces apoptosis in p53-Deficient H1299 cells. Advances in Pharmacological Sciences, 2014, 847574. [CrossRef]
  • 36. Jalilian, F., Moieni-Arya, M., Hosseinzadeh, L., Shokoohinia, Y. (2021). Oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin. Research in Pharmaceutical Sciences, 17(1), 12-21. [CrossRef]

PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EKSTRELERİNİN HEPG2 HÜCRELERİNDEKİ SİTOTOKSİK VE APOPTOTİK ETKİLERİNİN ARAŞTIRILMASI

Yıl 2024, Cilt: 48 Sayı: 1, 46 - 55, 20.01.2024
https://doi.org/10.33483/jfpau.1336857

Öz

Amaç: Bu çalışmanın amacı; Prangos Heyniae H. Duman & M. F. Watson kök ekstrelerinin insan karaciğer kanseri hücrelerindeki antikanser potansiyellerini araştırmak ve ekstre ile indüklenen sitotoksisitede rol oynayan moleküler mekanizmaları değerlendirmektir.
Gereç ve Yöntem: P. heyniae kök ekstrelerin HepG2 hücrelerinin canlılığına olan olası etkilerini araştırmak amacıyla hücreler kloroform, n-hekzan ya da metanol ekstreleri ile inkübe edildi. MTT testi ile IC50 değerlerinin belirlenmesi sonrası, selektivite indeksleri nedeniyle n-hekzan ve metanol ekstreleri ile çalışmaya devam edilmedi. Yapı-biyolojik aktivite ilişkisini kurabilmek amacıyla kloroform ekstresinin kimyasal karakterizasyonu HPLC analizi ile gerçekleştirildi. Kloroform ekstresinin mitokondriyal membran potansiyeli ve kaspaz-3 aktivasyonu üzerindeki etkileri ileri deneylerle araştırıldı. Ayrıca, sitotoksisitede rol oynayan hücre ölüm yolunun belirlenmesi amacıyla selektif inhibitörler varlığında hücre canlılığı ölçüldü.
Sonuç ve Tartışma: Kloroform ekstresi, HepG2 hücrelerinde canlılığın güçlü ve selektif inhibisyonuna neden oldu. Benzer sitotoksik etki n-hekzan ya da metanol ekstreleri ile saptanmadı. Kloroform ekstresi tarafından indüklenen sitotoksisite pan-kaspaz apoptoz inhibitörü varlığında önlendi. Ayrıca, HepG2 hücrelerinin kloroform ekstresi ile inkübasyonu, mitokondriyal membran potansiyelinde hasara ve kaspaz-3 aktivasyonuna neden oldu. Kloroform ekstresinin ana bileşenleri olarak oxypeucedanin, isoimperatorin ve osthole tespit edildi. Bu sonuçlar, kloroform ekstresindeki kumarin ve furanokumarin türevlerinin antikanser etki mekanizmasında apoptozun rol oynayabileceğini göstermektedir.

Destekleyen Kurum

Laboratuvarımızın mevcut imkanları ile projemiz yürütülmüştür.

Kaynakça

  • 1. Yang, J.D., Roberts, L.R. (2010). Epidemiology and management of hepatocellular carcinoma. Infectious Disease Clinics of North America, 24(4), 899-919. [CrossRef]
  • 2. Bousbaa, H. (2021). Novel anticancer strategies. Pharmaceutics, 13(2), 275. [CrossRef]
  • 3. Greenwell, M., Rahman, P.K. (2015). Medicinal plants: Their use in anticancer treatment. International Journal of Pharmaceutical Sciences and Research, 6(10), 4103-4112. [CrossRef]
  • 4. Newman, D.J., Cragg, G.M. (2012). Natural products as sources of new drugs over the 30 years from 1981 to 2010. Journal of Natural Products, 75(3), 311-335. [CrossRef]
  • 5. Lyskov, D.F., Degtjareva, G.V., Samigullin, T.H., Pimenov, M.G. (2017). The revision of Prangos subsections Koelzella and Fedtschenkoana (Apiaceae) with some notes to phylogeny and biogeography of the genus: Molecular and morphological evidence. Plant Systematics and Evolution, 303(7), 815-826. [CrossRef]
  • 6. Menemen, Y. (2012). Türkiye bitkileri listesi:(damarlı bitkiler) (1st ed.; A. Guner, ed.). İstanbul: Nezahat Gökyiğit Botanik Bahçesi Yayınları.
  • 7. Mottaghipisheh, J., Kiss, T., Tóth, B., Csupor, D. (2020). The Prangos genus: A comprehensive review on traditional use, phytochemistry, and pharmacological activities. Phytochemistry Reviews, 19, 1449-1470. [CrossRef]
  • 8. Farkhad, N.K., Farokhi, F., Tukmacki, A. (2012). Hydro-alcoholic extract of the root of Prangos ferulacea (L.) Lindl can improve serum glucose and lipids in alloxan-induced diabetic rats. Avicenna Journal of Phytomedicine, 2(4), 179.
  • 9. Albayrak, G., Demir, S., Koyu H., Baykan, S. (2022). Anticholinesterase and antityrosinase activities of endemic Prangos heyniae H. Duman and M.F. Watson and its metabolites. İstanbul Journal of Pharmacy, 53(1), 51-57. [CrossRef]
  • 10. Shokoohinia, Y., Sajjadi, S.E., Gholamzadeh, S., Fattahi, A., Behbahani, M. (2014). Antiviral and cytotoxic evaluation of coumarins from Prangos ferulacea. Pharmaceutical Biology, 52(12), 1543-1549. [CrossRef]
  • 11. Loizzo, M.R., Tundis, R., Menichini, F. (2008) Antiproliferative effects of essential oils and their major constituents inhuman renal adenocarcinoma and amelanotic melanoma cells. Cell Proliferation, 41, 1002-1012. [CrossRef]
  • 12. Razavi, S.M., Zarrini, G., Zahri, S., Mohammadi, S. (2010). Biological activity of Prangos uloptera DC. roots, a medicinal plant from Iran. Natural Product Research, 24(9), 797-803. [CrossRef]
  • 13. Yazici-Tutunis, S., Erucar, F.M., Oztas, E., Akalin, E., Ozhan, G., Miski, M., Tan, N. (2021). Chemical composition and cytotoxic effect of Prangos turcica. Records of Natural Products, 15(6), 503-512, [CrossRef]
  • 14. Sarghaleh, S.J., Behbahani, B.A., Hojjati, M.H. (2023). Evaluation of the constituent compounds, antioxidant, anticancer, and antimicrobial potential of Prangos ferulacea plant extract and its effect on Listeria monocytogenes virulence gene expression. Frontiers in Microbiology, 14, 1202228. [CrossRef]
  • 15. Ahmed, J., Güvenç, A., Küçükboyacı, N., Baldemir, A., Coşkun, M. (2011). Total phenolic contents and antioxidant activities of Prangos Lindl. (Umbelliferae) species growing in Konya province (Turkey) Turkish Journal of Biology, 35(3), 353-360 [CrossRef]
  • 16. Albayrak, G., Demir, S., Kose, F.A., Baykan, S. (2023). New coumarin glycosides from endemic Prangos heyniae H. Duman and M.F. Watson. Natural Product Research, 37(2), 227-239. [CrossRef]
  • 17. Tan, X. W., Xia, H., Xu, J.H., Cao, J.G. (2009). Induction of apoptosis in human liver carcinoma HepG2 cell line by 5-allyl-7-gen-difluoromethylenechrysin. World Journal of Gastroenterology, 15(18), 2234-2239. [CrossRef]
  • 18. Sevin, G., Alan, E., Demir, S., Albayrak, G., Demiroz, T., Yetik-Anacak, G., Baykan, S. (2022). Comparative evaluation of relaxant effects of three prangos species on mouse corpus cavernosum: Chemical characterization and the relaxant mechanisms of action of P. pabularia and (+)-oxypeucedanin. Journal of Ethnopharmacology, 284, 114823. [CrossRef]
  • 19. Sazonova, E.V., Chesnokov, M.S., Zhivotovsky, B., Kopeina, G.S. (2022). Drug toxicity assessment: Cell proliferation versus cell death. Cell Death Discovery, 8(1), 417. [CrossRef]
  • 20. Chalmers, S., McCarron, J.G. (2008). The mitochondrial membrane potential and Ca2+ oscillations in smooth muscle. Journal of Cell Science, 121, 75-85. [CrossRef]
  • 21. Ghosh, R., Goswami, S.K., Feitoza, L.F., Hammock, B., Gomes, A.V. (2016). Diclofenac induces proteasome and mitochondrial dysfunction in murine cardiomyocytes and hearts. International Journal of Cardiology, 223, 923-935. [CrossRef]
  • 22. Bashir, R., Ahmad Zargar, O., Hamid Dar, A., Yedukondalu, N., Parvaiz, Q., Hamid, R. (2022). The modulation of PI3K/Akt pathway by 3β hydroxylup-12-en-28-oic acid isolated from Thymus linearis induces cell death in HCT-116 cells. Chemical Biology and Drug Design, 99(1), 162-178. [CrossRef]
  • 23. Lee, Y.T., Wang, J.J., Luu, M., Noureddin, M., Kosari, K., Agopian, V.G., Rich, N.E., Lu, S.C., Tseng, H.R., Nissen, N.N., Singal, A.G., Yang, J.D. (2021). The Mortality and overall survival trends of primary liver cancer in the United States. Journal of the National Cancer Institute, 113(11), 1531-1541. [CrossRef]
  • 24. Yarchoan, M., Agarwal, P., Villanueva, A., Rao, S., Dawson, L.A., Lovet, J.M., Finn, R.S., Groopman, J.D., El-Serag, H.B., Monga, S.P., Wang, X.W., Karin, M., Schwartz, R.E., Tanabe, K.K., Roberts, L.R., Gunaratne, P.H., Tsung, A., Brown, K.A., Lawrence, T.S., Salem, R., Ahmed, M.M. (2019). Recent developments and therapeutic strategies against hepatocellular carcinoma. Cancer Research, 79(17), 4326-4330. [CrossRef]
  • 25. Singh, S., Sharma, B., Kanwar, S.S., Kumar, A. (2016). Lead phytochemicals for anticancer drug development. Frontiers in Plant Science. 7, 8973. [CrossRef]
  • 26. Kissin, I. (2013). An early indicator of drug success: Top journal selectivity index. Drug Design, Development, and Therapy, 7, 93-98. [CrossRef]
  • 27. Elmore, S. (2007). Apoptosis: A review of programmed cell death. Toxicologic Pathology, 35(4), 495-516. [CrossRef]
  • 28. Wang, C., Youle, R.J. (2009). The role of mitochondria in apoptosis. Annual Review of Genetics, 43, 95-118. [CrossRef]
  • 29. Park, W., Park, S., Song, G., Lim, W. (2019). Inhibitory effects of osthole on human breast cancer cell progression via induction of cell cycle arrest, mitochondrial dysfunction, and ER stress. Nutrients, 11(11), 2777. [CrossRef]
  • 30. Liang, J., Zhou, J., Xu, Y., Huang, X., Wang, X., Huang, W., Li, H. (2020). Osthole inhibits ovarian carcinoma cells through LC3-mediated autophagy and GSDME-dependent pyroptosis except for apoptosis. European Journal of Pharmacology, 874, 172990. [CrossRef]
  • 31. Bae, H., Lee, J.Y., Song, J., Song, G., Lim, W. (2021). Osthole interacts with an ER-mitochondria axis and facilitates tumor suppression in ovarian cancer. Journal of Cellular Physiology, 236(2), 1025-1042. [CrossRef]
  • 32. Choudhary, G.S., Al-Harbi, S., Almasan, A. (2015). Caspase-3 activation is a critical determinant of genotoxic stress-induced apoptosis. Methods in Molecular Biology, 1219, 1-9. [CrossRef]
  • 33. Kang, T.J., Lee, S.Y., Singh, R.P., Agarwal, R., Yim, D.S. (2009). Anti-tumor activity of oxypeucedanin from Ostericum koreanum against human prostate carcinoma DU145 cells. Acta Oncologica, 48(6), 895-900. [CrossRef]
  • 34. Ye, J., Sun, D., Yu, Y., Yu, J. (2020). Osthole resensitizes CD133+ hepatocellular carcinoma cells to cisplatin treatment via PTEN/AKT pathway. Aging, 12(14), 14406-14417. [CrossRef]
  • 35. Shokoohinia, Y., Hosseinzadeh, L., Alipour, M., Mostafaie, A., Mohammadi-Motlagh, H.R. (2014). Comparative evaluation of cytotoxic and apoptogenic effects of several coumarins on human cancer cell lines: osthole induces apoptosis in p53-Deficient H1299 cells. Advances in Pharmacological Sciences, 2014, 847574. [CrossRef]
  • 36. Jalilian, F., Moieni-Arya, M., Hosseinzadeh, L., Shokoohinia, Y. (2021). Oxypeucedanin and isoimperatorin extracted from Prangos ferulacea (L.) Lindl protect PC12 pheochromocytoma cells from oxidative stress and apoptosis induced by doxorubicin. Research in Pharmaceutical Sciences, 17(1), 12-21. [CrossRef]

Ayrıntılar

Birincil Dil İngilizce
Konular Farmasotik Toksikoloji
Bölüm Araştırma Makalesi
Yazarlar

Ege ARZUK 0000-0002-3239-4855

Gökay ALBAYRAK 0000-0002-5729-0796

Ali ERGÜÇ 0000-0002-9791-4399

Ecrin ATIŞ 0009-0001-8475-8067

İclal TAN 0009-0001-2006-5480

Şüra BAYKAN 0000-0002-3624-4811

Erken Görünüm Tarihi 18 Ekim 2023
Yayımlanma Tarihi 20 Ocak 2024
Gönderilme Tarihi 2 Ağustos 2023
Kabul Tarihi 5 Ekim 2023
Yayımlandığı Sayı Yıl 2024 Cilt: 48 Sayı: 1

Kaynak Göster

APA ARZUK, E., ALBAYRAK, G., ERGÜÇ, A., ATIŞ, E., vd. (2024). INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS. Journal of Faculty of Pharmacy of Ankara University, 48(1), 46-55. https://doi.org/10.33483/jfpau.1336857
AMA ARZUK E, ALBAYRAK G, ERGÜÇ A, ATIŞ E, TAN İ, BAYKAN Ş. INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS. Ankara Ecz. Fak. Derg. Ocak 2024;48(1):46-55. doi:10.33483/jfpau.1336857
Chicago ARZUK, Ege, Gökay ALBAYRAK, Ali ERGÜÇ, Ecrin ATIŞ, İclal TAN, ve Şüra BAYKAN. “INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS”. Journal of Faculty of Pharmacy of Ankara University 48, sy. 1 (Ocak 2024): 46-55. https://doi.org/10.33483/jfpau.1336857.
EndNote ARZUK E, ALBAYRAK G, ERGÜÇ A, ATIŞ E, TAN İ, BAYKAN Ş (01 Ocak 2024) INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS. Journal of Faculty of Pharmacy of Ankara University 48 1 46–55.
IEEE E. ARZUK, G. ALBAYRAK, A. ERGÜÇ, E. ATIŞ, İ. TAN, ve Ş. BAYKAN, “INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS”, Ankara Ecz. Fak. Derg., c. 48, sy. 1, ss. 46–55, 2024, doi: 10.33483/jfpau.1336857.
ISNAD ARZUK, Ege vd. “INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS”. Journal of Faculty of Pharmacy of Ankara University 48/1 (Ocak 2024), 46-55. https://doi.org/10.33483/jfpau.1336857.
JAMA ARZUK E, ALBAYRAK G, ERGÜÇ A, ATIŞ E, TAN İ, BAYKAN Ş. INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS. Ankara Ecz. Fak. Derg. 2024;48:46–55.
MLA ARZUK, Ege vd. “INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS”. Journal of Faculty of Pharmacy of Ankara University, c. 48, sy. 1, 2024, ss. 46-55, doi:10.33483/jfpau.1336857.
Vancouver ARZUK E, ALBAYRAK G, ERGÜÇ A, ATIŞ E, TAN İ, BAYKAN Ş. INVESTIGATION OF CYTOTOXIC AND APOPTOTIC EFFECTS OF PRANGOS HEYNIAE H. DUMAN & M. F. WATSON EXTRACTS ON HEPG2 CELLS. Ankara Ecz. Fak. Derg. 2024;48(1):46-55.

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.