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ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID

Yıl 2024, Cilt: 48 Sayı: 1, 197 - 204, 20.01.2024
https://doi.org/10.33483/jfpau.1399176

Öz

Objective: Lavandula angustifolia L. (Lavender) is one of the most plants essential oils used in the cosmetic, food and biological activities. Kojic acid has been used in cosmetics for its whitening effect and pharmaceutical activity. The aim of this study to determine the composition of the Pharmacopoeia quality L. angustifolia essential oil (EO). Moreover, antimicrobial activities against skin pathogens and synergistic antibacterial activity were also examined of EO and kojic acid.
Material and Method: In this work, chemical composition of the EO was defined. Linalyl acetate (43.3%) and linalool (38.6%) were determined as the major components by GC-MS and GC-FID, simultaneously. The antimicrobial activity was evaluated against Candida albicans, C. glabrata, Staphylococcus aureus and Salmonella typhimurium.
Result and Discussion: EO and kojic acid showed weak antimicrobial effects. MIC values were determined as the EO 10 mg/ml and kojic acid 1.25 mg/ml against S. typhimurium. To assess the synergistic activity was evaluated by the checkerboard microdilution assay, EO was combined with kojic acid against S. typhimurium. Among the tested skin pathogen microorganisms, S. typhimurium was more sensitive to kojic acid. Therefore, synergic activity was investigated against S. typhimurium and found indifferent effect.

Etik Beyan

The authors declare that the ethics committee approval is not required for this study.

Destekleyen Kurum

Laber Kimya and Sns Gıda and Kozmetik

Teşekkür

The authors would like to thank Prof. Dr. Fatih Demirci (Anadolu University, Eskisehir) for supporting this work and the Laber Kimya and Sns Gıda and Kozmetik for financial supports.

Kaynakça

  • 1. Yap, P.S.X., Yiap, B.C., Ping, H.C., Lim, S.H.E. (2014). Essential oils, a new horizon in combating bacterial antibiotic resistance. The Open Microbiology Journal, 8, 6-14. [CrossRef]
  • 2. Willing, B.P., Pepin, D.M., Marcolla, C.S., Forgie, A.J., Diether, N.E., Bourrie, B.C. (2018). Bacterial resistance to antibiotic alternatives: a wolf in sheep’s clothing? Animal Frontiers, 8(2), 39-47. [CrossRef]
  • 3. Cardia, G.F.E., Silva-Filho, S.E., Silva, E.L., Uchida, N.S., Cavalcante, H.A.O., Cassarotti, L.L., Salvadego, V.E.C., Spironello, R.A., Bersani-Amado, C.A., Cuman, R.K.N. (2018). Effect of lavender (lavandula angustifolia) essential oil on acute inflammatory response. Evidence-based Complementary and Alternative Medicine, 2018, 1413940. [CrossRef]
  • 4. Adaszyńska-Skwirzyńska, M., Szczerbińska, D. (2018). The antimicrobial activity of lavender essential oil (Lavandula angustifolia) and its influence on the production performance of broiler chickens. Journal of Animal Physiology and Animal Nutrition, 102(4), 1020-1025. [CrossRef]
  • 5. Wu, Y., Shi, Y.G., Zeng, L.Y., Pan, Y., Huang, X.Y., Bian, L.Q., Zhu, Y.J., Zhang, R.R., Zhang, J. (2019). Evaluation of antibacterial and anti-biofilm properties of kojic acid against five food-related bacteria and related subcellular mechanisms of bacterial inactivation. Food Science and Technology International, 25(1), 3-15. [CrossRef]
  • 6. Kim, J.H., Chang, P.K., Chan, K.L., Faria, N.C., Mahoney, N., Kim, Y.K., Martins, M.L., Campbell, B.C. (2012). Enhancement of commercial antifungal agents by kojic Acid. International Journal of Molecular Sciences, 13(11), 13867-13880. [CrossRef]
  • 7. Kılıç, C.S., Demirci, B., Kırcı, D., Duman, H., Gürbüz, İ. (2023). Essential oils of ferulago glareosa kandemir & hedge roots and aerial parts: pca and hca analyses. Chemistry & Biodiversity, 20(5), e202300364. [CrossRef]
  • 8. McLafferty, F.W., Stauffer, D.B. (1989). The Wiley/NBS Registry of Mass Spectral Data, Wiley and Sons, New York.
  • 9. Hochmuth, D.H. (2008). MassFinder 4.0, Hochmuth Scientific Consulting, Hamburg, Germany.
  • 10. Clinical and Laboratory Standards Institute (CLSI). (2006). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically CLSI M7-A7, Clinical and Laboratory Standards Institute, West Valley Road, Wayne, Pennsylvania, USA.
  • 11. Clinical and Laboratory Standards Institute (CLSI). (2002). Methods for dilution antifungal susceptibility testing of yeast that grow aerobically CLSI M27-A2, Clinical and Laboratory Standards Institute, West Valley Road, Wayne, Pennsylvania, USA.
  • 12. Göger, G., Demirci, B., Ilgın, S., Demirci, F. (2018). Antimicrobial and toxicity profiles evaluation of the chamomile (Matricaria recutita L.) essential oil combination with standard antimicrobial agents. Industrial Crops and Products, 120, 279-285. [CrossRef]
  • 13. De Rapper, S., Van Vuuren, S.F., Kamatou, G.P., Viljoen, A.M., Dagne, E. (2012). The additive and synergistic antimicrobial effects of select frankincense and myrrh oils-a combination from the pharaonic pharmacopoeia. Letters in Applied Microbiology, 54(4), 352-358. [CrossRef]
  • 14. Pokajewicz, K., Białoń, M., Svydenko, L., Fedin, R., Hudz, N. (2021). Chemical composition of the essential oil of the new cultivars of Lavandula angustifolia Mill. Bred in Ukraine. Molecules, 26(18), 5681. [CrossRef]
  • 15. Kozuharova, E., Simeonov, V., Batovska, D., Stoycheva, C., Valchev, H., Benbassat, N. (2023). Chemical composition and comparative analysis of lavender essential oil samples from Bulgaria in relation to the pharmacological effects. Pharmacia, 70(2), 395-403. [CrossRef]
  • 16. Wu, Y., Shi, Y.G., Zeng, L.Y., Pan, Y., Huang, X.Y., Bian, L.Q., Zhu, Y.J., Zhang, R.R., Zhang, J. (2019). Evaluation of antibacterial and anti-biofilm properties of kojic acid against five food-related bacteria and related subcellular mechanisms of bacterial inactivation. Food Science and Technology International, 25(1), 3-15. [CrossRef]
  • 17. Zilles, J.C., Dos Santos, F.L., Kulkamp‐Guerreiro, I.C., Contri, R.V. (2022). Biological activities and safety data of kojic acid and its derivatives: A review. Experimental Dermatology, 31(10), 1500-1521. [CrossRef]
  • 18. Walasek-Janusz, M., Grzegorczyk, A., Zalewski, D., Malm, A., Gajcy, S., Gruszecki, R. (2022). Variation in the antimicrobial activity of essential oils from cultivars of Lavandula angustifolia and L.× intermedia. Agronomy, 12(12), 2955. [CrossRef]
  • 19. Gismondi, A., Di Marco, G., Redi, E.L., Ferrucci, L., Cantonetti, M., Canini, A. (2021). The antimicrobial activity of Lavandula angustifolia Mill. essential oil against Staphylococcus species in a hospital environment. Journal of Herbal Medicine, 26, 100426. [CrossRef]
  • 20. Wang, R., Hu, X., Agyekumwaa, A.K., Li, X., Xiao, X., Yu, Y. (2021). Synergistic effect of kojic acid and tea polyphenols on bacterial inhibition and quality maintenance of refrigerated sea bass (Lateolabrax japonicus) fillets. LWT, 137, 110452. [CrossRef]
  • 21. Sun, R., Casali, L., Turner, R.J., Braga, D., Grepioni, F. (2023). Exploring the co-crystallization of kojic acid with silver (I), copper (II), zinc (II), and gallium (III) for potential antibacterial applications. Molecules, 28(3), 1244. [CrossRef]
  • 22. Song, L., Xie, W., Zhao, Y., Lv, X., Yang, H., Zeng, Q., Zeng, Z., Yang, X. (2019). Synthesis, antimicrobial, moisture absorption and retention activities of kojic acid-grafted konjac glucomannan oligosaccharides. Polymers, 11(12), 1979. [CrossRef]
  • 23. Karaca, N., Şener, G., Demirci, B., Demirci, F. (2021). Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor. Zeitschrift für Naturforschung C, 76(3-4), 169-173. [CrossRef]
  • 24. Moussii, I.M., Nayme, K., Timinouni, M., Jamaleddine, J., Filali, H., Hakkou, F. (2020). Synergistic antibacterial effects of Moroccan Artemisia herba alba, Lavandula angustifolia and Rosmarinus officinalis essential oils. Synergy, 10, 100057. [CrossRef]

KOJİK ASİT İLE LAVANDULA ANGUSTIFOLIA L. UÇUCU YAĞININ ANTİMİKROBİYAL KOMBİNASYONU

Yıl 2024, Cilt: 48 Sayı: 1, 197 - 204, 20.01.2024
https://doi.org/10.33483/jfpau.1399176

Öz

Amaç: Lamiaceae familyasına ait bir bitki olan Lavandula angustifolia L. (Lavanta), kozmetik, yiyecek ve biyolojik etkilerinden dolayı en çok kullanılan uçucu yağlardan birisidir. Kojik asit beyazlatıcı etkisi için kozmetikte ve antimikrobiyal, antibakteriyel, antiviral gibi farmasötik etkilerinden dolayı kullanılmaktadır. Bu çalışmanın amacı Farmakope kalitesindeki Lavandula angustifolia uçucu yağının kimyasal kompozisyonunu belirlemektir. Buna ek olarak cilt patojenlerine karşı antimikrobiyal aktivitesi ve kojik asit ile uçucu yağın aktivite olarak antibakteriyel sinerjisi incelenmiştir.
Gereç ve Yöntem: Bu çalışmada, uçucu yağın kimyasal bileşimi araştırılmıştır. Uçucu yağın içerdiği ana bileşenler GK-AİD ve GK-KS ile linalil asetat (%43,3) ve linalol (%38,6) olarak tespit edilmiştir. Antimikrobiyal aktivite Candida albicans, C. glabrata, Staphylococcus aureus ve Salmonella typhimurium'a karşı değerlendirildi.
Sonuç ve Tartışma: Uçucu yağ ve kojik asit C. albicans ve S. aureus'a karşı zayıf antimikrobiyal etki gösterdi. MİK değerleri S. typhimurium'a karşı uçucu yağda 10 mg/ml ve kojik asit 1,25 mg/ml olarak hesaplandı. Sinerjik aktiviteyi değerlendirmek için dama tahtası mikrodilüsyon yöntemi ile uçucu yağ kojik asit ile S. typhimurium'a karşı birleştirildi. Test edilen cilt patojen mikroorganizmalar arasında S. typhimurium, kojik aside karşı daha duyarlıdır. Bu nedenle S. typhimurium'a karşı sinerjik aktivite araştırıldı ve bağımsız etki gözlemlenmiştir.

Kaynakça

  • 1. Yap, P.S.X., Yiap, B.C., Ping, H.C., Lim, S.H.E. (2014). Essential oils, a new horizon in combating bacterial antibiotic resistance. The Open Microbiology Journal, 8, 6-14. [CrossRef]
  • 2. Willing, B.P., Pepin, D.M., Marcolla, C.S., Forgie, A.J., Diether, N.E., Bourrie, B.C. (2018). Bacterial resistance to antibiotic alternatives: a wolf in sheep’s clothing? Animal Frontiers, 8(2), 39-47. [CrossRef]
  • 3. Cardia, G.F.E., Silva-Filho, S.E., Silva, E.L., Uchida, N.S., Cavalcante, H.A.O., Cassarotti, L.L., Salvadego, V.E.C., Spironello, R.A., Bersani-Amado, C.A., Cuman, R.K.N. (2018). Effect of lavender (lavandula angustifolia) essential oil on acute inflammatory response. Evidence-based Complementary and Alternative Medicine, 2018, 1413940. [CrossRef]
  • 4. Adaszyńska-Skwirzyńska, M., Szczerbińska, D. (2018). The antimicrobial activity of lavender essential oil (Lavandula angustifolia) and its influence on the production performance of broiler chickens. Journal of Animal Physiology and Animal Nutrition, 102(4), 1020-1025. [CrossRef]
  • 5. Wu, Y., Shi, Y.G., Zeng, L.Y., Pan, Y., Huang, X.Y., Bian, L.Q., Zhu, Y.J., Zhang, R.R., Zhang, J. (2019). Evaluation of antibacterial and anti-biofilm properties of kojic acid against five food-related bacteria and related subcellular mechanisms of bacterial inactivation. Food Science and Technology International, 25(1), 3-15. [CrossRef]
  • 6. Kim, J.H., Chang, P.K., Chan, K.L., Faria, N.C., Mahoney, N., Kim, Y.K., Martins, M.L., Campbell, B.C. (2012). Enhancement of commercial antifungal agents by kojic Acid. International Journal of Molecular Sciences, 13(11), 13867-13880. [CrossRef]
  • 7. Kılıç, C.S., Demirci, B., Kırcı, D., Duman, H., Gürbüz, İ. (2023). Essential oils of ferulago glareosa kandemir & hedge roots and aerial parts: pca and hca analyses. Chemistry & Biodiversity, 20(5), e202300364. [CrossRef]
  • 8. McLafferty, F.W., Stauffer, D.B. (1989). The Wiley/NBS Registry of Mass Spectral Data, Wiley and Sons, New York.
  • 9. Hochmuth, D.H. (2008). MassFinder 4.0, Hochmuth Scientific Consulting, Hamburg, Germany.
  • 10. Clinical and Laboratory Standards Institute (CLSI). (2006). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically CLSI M7-A7, Clinical and Laboratory Standards Institute, West Valley Road, Wayne, Pennsylvania, USA.
  • 11. Clinical and Laboratory Standards Institute (CLSI). (2002). Methods for dilution antifungal susceptibility testing of yeast that grow aerobically CLSI M27-A2, Clinical and Laboratory Standards Institute, West Valley Road, Wayne, Pennsylvania, USA.
  • 12. Göger, G., Demirci, B., Ilgın, S., Demirci, F. (2018). Antimicrobial and toxicity profiles evaluation of the chamomile (Matricaria recutita L.) essential oil combination with standard antimicrobial agents. Industrial Crops and Products, 120, 279-285. [CrossRef]
  • 13. De Rapper, S., Van Vuuren, S.F., Kamatou, G.P., Viljoen, A.M., Dagne, E. (2012). The additive and synergistic antimicrobial effects of select frankincense and myrrh oils-a combination from the pharaonic pharmacopoeia. Letters in Applied Microbiology, 54(4), 352-358. [CrossRef]
  • 14. Pokajewicz, K., Białoń, M., Svydenko, L., Fedin, R., Hudz, N. (2021). Chemical composition of the essential oil of the new cultivars of Lavandula angustifolia Mill. Bred in Ukraine. Molecules, 26(18), 5681. [CrossRef]
  • 15. Kozuharova, E., Simeonov, V., Batovska, D., Stoycheva, C., Valchev, H., Benbassat, N. (2023). Chemical composition and comparative analysis of lavender essential oil samples from Bulgaria in relation to the pharmacological effects. Pharmacia, 70(2), 395-403. [CrossRef]
  • 16. Wu, Y., Shi, Y.G., Zeng, L.Y., Pan, Y., Huang, X.Y., Bian, L.Q., Zhu, Y.J., Zhang, R.R., Zhang, J. (2019). Evaluation of antibacterial and anti-biofilm properties of kojic acid against five food-related bacteria and related subcellular mechanisms of bacterial inactivation. Food Science and Technology International, 25(1), 3-15. [CrossRef]
  • 17. Zilles, J.C., Dos Santos, F.L., Kulkamp‐Guerreiro, I.C., Contri, R.V. (2022). Biological activities and safety data of kojic acid and its derivatives: A review. Experimental Dermatology, 31(10), 1500-1521. [CrossRef]
  • 18. Walasek-Janusz, M., Grzegorczyk, A., Zalewski, D., Malm, A., Gajcy, S., Gruszecki, R. (2022). Variation in the antimicrobial activity of essential oils from cultivars of Lavandula angustifolia and L.× intermedia. Agronomy, 12(12), 2955. [CrossRef]
  • 19. Gismondi, A., Di Marco, G., Redi, E.L., Ferrucci, L., Cantonetti, M., Canini, A. (2021). The antimicrobial activity of Lavandula angustifolia Mill. essential oil against Staphylococcus species in a hospital environment. Journal of Herbal Medicine, 26, 100426. [CrossRef]
  • 20. Wang, R., Hu, X., Agyekumwaa, A.K., Li, X., Xiao, X., Yu, Y. (2021). Synergistic effect of kojic acid and tea polyphenols on bacterial inhibition and quality maintenance of refrigerated sea bass (Lateolabrax japonicus) fillets. LWT, 137, 110452. [CrossRef]
  • 21. Sun, R., Casali, L., Turner, R.J., Braga, D., Grepioni, F. (2023). Exploring the co-crystallization of kojic acid with silver (I), copper (II), zinc (II), and gallium (III) for potential antibacterial applications. Molecules, 28(3), 1244. [CrossRef]
  • 22. Song, L., Xie, W., Zhao, Y., Lv, X., Yang, H., Zeng, Q., Zeng, Z., Yang, X. (2019). Synthesis, antimicrobial, moisture absorption and retention activities of kojic acid-grafted konjac glucomannan oligosaccharides. Polymers, 11(12), 1979. [CrossRef]
  • 23. Karaca, N., Şener, G., Demirci, B., Demirci, F. (2021). Synergistic antibacterial combination of Lavandula latifolia Medik. essential oil with camphor. Zeitschrift für Naturforschung C, 76(3-4), 169-173. [CrossRef]
  • 24. Moussii, I.M., Nayme, K., Timinouni, M., Jamaleddine, J., Filali, H., Hakkou, F. (2020). Synergistic antibacterial effects of Moroccan Artemisia herba alba, Lavandula angustifolia and Rosmarinus officinalis essential oils. Synergy, 10, 100057. [CrossRef]

Ayrıntılar

Birincil Dil İngilizce
Konular Farmakognozi
Bölüm Araştırma Makalesi
Yazarlar

Damla KIRCI 0000-0002-3479-3999

Özge ÖZŞEN BATUR 0000-0002-6908-1174

Betül DEMİRCİ 0000-0003-2343-746X

Erken Görünüm Tarihi 25 Aralık 2023
Yayımlanma Tarihi 20 Ocak 2024
Gönderilme Tarihi 4 Aralık 2023
Kabul Tarihi 18 Aralık 2023
Yayımlandığı Sayı Yıl 2024 Cilt: 48 Sayı: 1

Kaynak Göster

APA KIRCI, D., ÖZŞEN BATUR, Ö., & DEMİRCİ, B. (2024). ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID. Journal of Faculty of Pharmacy of Ankara University, 48(1), 197-204. https://doi.org/10.33483/jfpau.1399176
AMA KIRCI D, ÖZŞEN BATUR Ö, DEMİRCİ B. ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID. Ankara Ecz. Fak. Derg. Ocak 2024;48(1):197-204. doi:10.33483/jfpau.1399176
Chicago KIRCI, Damla, Özge ÖZŞEN BATUR, ve Betül DEMİRCİ. “ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID”. Journal of Faculty of Pharmacy of Ankara University 48, sy. 1 (Ocak 2024): 197-204. https://doi.org/10.33483/jfpau.1399176.
EndNote KIRCI D, ÖZŞEN BATUR Ö, DEMİRCİ B (01 Ocak 2024) ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID. Journal of Faculty of Pharmacy of Ankara University 48 1 197–204.
IEEE D. KIRCI, Ö. ÖZŞEN BATUR, ve B. DEMİRCİ, “ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID”, Ankara Ecz. Fak. Derg., c. 48, sy. 1, ss. 197–204, 2024, doi: 10.33483/jfpau.1399176.
ISNAD KIRCI, Damla vd. “ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID”. Journal of Faculty of Pharmacy of Ankara University 48/1 (Ocak 2024), 197-204. https://doi.org/10.33483/jfpau.1399176.
JAMA KIRCI D, ÖZŞEN BATUR Ö, DEMİRCİ B. ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID. Ankara Ecz. Fak. Derg. 2024;48:197–204.
MLA KIRCI, Damla vd. “ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID”. Journal of Faculty of Pharmacy of Ankara University, c. 48, sy. 1, 2024, ss. 197-04, doi:10.33483/jfpau.1399176.
Vancouver KIRCI D, ÖZŞEN BATUR Ö, DEMİRCİ B. ANTIMICROBIAL COMBINATION OF LAVANDULA ANGUSTIFOLIA L. ESSENTIAL OIL WITH KOJIC ACID. Ankara Ecz. Fak. Derg. 2024;48(1):197-204.

Kapsam ve Amaç

Ankara Üniversitesi Eczacılık Fakültesi Dergisi, açık erişim, hakemli bir dergi olup Türkçe veya İngilizce olarak farmasötik bilimler alanındaki önemli gelişmeleri içeren orijinal araştırmalar, derlemeler ve kısa bildiriler için uluslararası bir yayım ortamıdır. Bilimsel toplantılarda sunulan bildiriler supleman özel sayısı olarak dergide yayımlanabilir. Ayrıca, tüm farmasötik alandaki gelecek ve önceki ulusal ve uluslararası bilimsel toplantılar ile sosyal aktiviteleri içerir.