İNCİR ÇEKİRDEĞİ YAĞININ KOLOREKTAL KANSER HÜCRE HATLARI ÜZERİNDEKİ SİTOTOKSİK VE ANTİ-İNVAZİV ETKİLERİ

Yıl 2025, Cilt: 49 Sayı: 3, 878 - 887, 19.09.2025

Zeynep Doğru , Mehmet Akbulut , Hüsamettin Vatansev , Serdar Karakurt

https://doi.org/10.33483/jfpau.1650925

Öz

Amaç: Kolorektal kanser (KRC), dünya genelinde yüksek morbidite ve mortaliteye sahip yaygın bir malignitedir. Cerrahi ve kemoterapi gibi geleneksel tedavilerin yanında, doğal kaynaklı biyoaktif bileşiklerin destekleyici terapötik potansiyelleri üzerine çalışmalar giderek artmaktadır. Bu çalışma, incir (Ficus carica) çekirdeği yağının HT-29 ve DLD-1 kolorektal kanser hücre hatlarında proliferasyon ve invazyon üzerindeki etkilerinin in vitro koşullarda değerlendirilmesini amaçlamaktadır.
Gereç ve Yöntem: İncir çekirdeği yağının kimyasal bileşimi GC-FID ve HPLC analizleri ile karakterize edilmiştir. Sitotoksik etkiler Alamar Blue testi ile değerlendirilmiş ve IC₅₀ değerleri hesaplanmıştır; invazyon kapasitesi ise Matrigel transwell invazyon analizi ile belirlenmiştir.
Sonuç ve Tartışma: Analiz sonuçlarına göre, incir çekirdeği yağı yüksek düzeyde α-linolenik asit (%39.97), linoleik asit (%33.24), oleik asit (%16.71) ve γ-tokoferol (4800 mg/kg) içermektedir. Yağ, HT-29 ve DLD-1 hücrelerinde doza bağlı antiproliferatif etki göstermiş, sağlıklı PNT1A hücrelerinde ise daha düşük toksisite sergilemiştir. Ayrıca, her iki kanser hücre hattında anlamlı düzeyde invazyon inhibisyonu saptanmıştır. Bulgular, incir çekirdeği yağının antikanser potansiyel taşıyan doğal bir ajan olabileceğini göstermektedir.

Anahtar Kelimeler

İncir çekirdeği yağı , invazyon , kolorektal kanser , sitotoksisite , γ-tokoferol

Etik Beyan

Bu çalışma, uluslararası bilimsel ve etik standartlara uygun olarak yürütülmüş olup, yalnızca in vitro hücre kültürü deneylerine dayanmaktadır. İnsan veya hayvan denekler üzerinde herhangi bir uygulama içermediği için, ilgili mevzuat ve etik yönergeler çerçevesinde etik kurul onayı gerektirmemektedir.

Destekleyen Kurum

Selçuk Üniversitesi

Proje Numarası

16202041

Teşekkür

Bu araştırma Selçuk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından desteklenmiştir.

EFFECTS OF FIG SEED OIL ON THE PROLIFERATION AND INVASION OF COLORECTAL CANCER CELL LINES

Öz

Objective: Colorectal cancer (CRC) is a common malignancy associated with high morbidity and mortality worldwide. In addition to conventional treatments such as surgery and chemotherapy, there is a growing interest in the therapeutic potential of naturally derived bioactive compounds. This study aims to evaluate the in vitro effects of fig (Ficus carica) seed oil on the proliferation and invasion of HT-29 and DLD-1 colorectal cancer cell lines.
Material and Method: The chemical composition of fig seed oil was characterized by GC-FID and HPLC analyses. Cytotoxic effects were assessed using the Alamar Blue assay, and IC₅₀ values were calculated. Invasion capacity was determined through a Matrigel-transwell invasion assay.
Result and Discussion: According to the analysis results, fig seed oil was found to be rich in α-linolenic acid (39.97%), linoleic acid (33.24%), oleic acid (16.71%), and γ-tocopherol (4800 mg/kg). The oil exhibited dose-dependent antiproliferative activity on HT-29 and DLD-1 cells, while demonstrating lower toxicity on healthy PNT1A cells. Furthermore, a significant inhibition of invasion was observed in both cancer cell lines. These findings suggest that fig seed oil may serve as a natural agent with anticancer potential for use in CRC management.

Anahtar Kelimeler

Colorectal cancer , cytotoxicity , fig seed oil , invasion , γ-tocopherol

Proje Numarası

16202041

Kaynakça

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