Evaluation of Porous Film – Peptide Nanoparticle Hybrid Structures as Drug-Releasing Wound Dressing Material

Öz

Breath Figure (BF), which is used to create patterns and pores on polymeric surfaces, is a polymer lithography technique. It is preferred in the production of biomaterials, especially porous membranes, wound dressings and tissue scaffolds, thanks to its advantages such as low technological cost, environmental friendliness and non-toxic chemicals. Biodegradable and biocompatible polyurethanes (PU) are the leading polymers used in biomaterial fabrication. In this study, porous PU films are fabricated with the BF method. Curcumin-loaded diphenylalanine (FFA/Ccm) peptide nanoparticles are integrated into the pores of films. Peptide nanoparticles are frequently used in biomedical applications, especially in drug release, thanks to their biocompatibility, chemical modifiability, inexpensive synthesis, and thermal and chemical stability. Curcumin is a natural phenolic compound. It is used as an active agent in wound treatments due to its pharmacological activities such as antioxidant, anti-inflammatory, antibacterial and antiviral. Within the scope of experimental studies, PU films produced with different BF parameters were characterized using light microscopy and scanning electron microscopy (SEM). The most suitable candidate for wound dressing material was evaluated in terms of their morphological features. Characterization of FFA/Ccm NPs was performed by attenuated total reflectance – Fourier transfrom infrared (ATR-FTIR) spectroscopy and SEM. Curcumin loading kinetics of nanoparticles were evaluated by UV-visible spectrophotometry. Morphological analyzes of the hybrid wound dressing obtained by embedding NPs in the pores of the PU film were investigated by SEM. Curcumin release kinetics of hybrid wound dressing was investigated by UV-visible spectrophotometry. Finally, the cytotoxicity of the hybrid wound dressing was evaluated by the MTT method. The use of the developed hybrid material as a wound dressing in biomedical applications is promising.

Anahtar Kelimeler

• polyurethane
• breath figure
• curcumin
• diphenylalaninamide
• peptide nanoparticle

Gözenekli Film – Peptit Nanopartikül Hibrit Yapıların İlaç Salımı Yapan Yara Örtü Malzemesi Olarak Değerlendirilmesi

Öz

Nefes figürü (Breath Figure) ile polimerik yüzeylerde desen ve gözenek oluşturma yöntemi oldukça yeni olup, bir polimer litografi tekniği olarak literatürde yerini almıştır. Teknolojik maliyetinin düşük olması, çevre dostu olması ve toksik kimyasallar kullanılmaması gibi avantajları sayesinde biyomalzeme üretiminde özellikle gözenekli membran, yara örtüleri ve doku iskelelerinin üretiminde tercih edilmektedir. Biyouyumlu ve biyobozunur poliüretanlar biyomalzeme üretiminde kullanılan polimerlerin başında gelmektedir. Çalışmada, nefes figürü tekniği ile üretilen poliüretan filmlerin gözenekleri içine kurkumin yüklü difenilalaninamid peptit nanopartiküller entegre edilerek bir hibrit yara örtü malzemesi dizayn edilmiştir. Biyouyumluluğu, kimyasal olarak modifiye edilebilirliği, kolay ve ucuz sentezlenebilirliği, olağanüstü̈ dayanıklılığı, termal ve kimyasal stabiliteleri sayesinde peptit nanopartiküller biyomedikal uygulamalarda özellikle ilaç salım uygulamalarında sıklıkla kullanılmaktadır. Kurkumin düşük molekül ağırlıklı bir fenolik bitki bileşenidir. Antioksidan, antienflamatuar, antibakteriyel ve antiviral gibi farmakolojik özelliklerinden dolayı yara tedavilerinde etken madde olarak kullanılmaktadır. Deneysel çalışmalar kapsamında, farklı BF parametreleri ile üretilen poliüretan filmler ışık mikroskobu ve taramalı elektron mikroskobu (SEM) kullanılarak karakterize edilmiş ve morfolojik özellikleri bakımından yara örtü malzemesi olmaya en uygun aday seçilmiştir. Hazırlanan kurkumin yüklü peptit (FFA/Ccm) nanopartiküller zayıflatılmış toplam yansıma – Fourier dönüşümlü kızılötesi (ATR-FTIR) spektroskopisi ve elektron mikroskopisi ile karakterize edilmiştir. FFA/Ccm nanopartiküllerin kurkumin yükleme kinetikleri UV-görünür bölge spektrofotometrisi ile değerlendirilmiştir. Poliüretan filmin gözenekleri içine FFA/Ccm nanopartiküllerin gömülmesi ile elde edilen hibrit yara örtüsünün morfolojik analizleri SEM ile ve kurkumin salım kinetikleri UV-görünür bölge spektrofotometrisi ile incelenmiştir. Son olarak, hibrit yara örtüsünün sitotoksisite testleri L929 fare fibroblast hücre hattında MTT yöntemiyle değerlendirilmiştir. Geliştirilen hibrit yara örtü malzemesinin biyomedikal uygulamalarda kullanımı gelecek vadetmektedir.

Anahtar Kelimeler

• poliüretan
• nefes figürü
• difenilalaninamid
• kurkumin
• peptit nanopartikül

Kaynakça

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