Ziziphus Jujube'nin Melanom Hücreleri Üzerindeki Potansiyel Antioksidan Etkileri

Öz

Hücresel redoks homeostazı, iç veya dış stres faktörlerine maruz kaldığında tehlikeye girerek oksidanların üstünlüğüne neden olur. Bu reaktif oksijen türlerinin diyabet ve kanser gibi birçok ciddi hastalığın etiyolojisinde kritik rol oynadığı bilinmektedir. Melanom, cilt kanserinin en ölümcül şeklidir ve oranları şu anda diğer önlenebilir kanserlerden daha hızlı artmaktadır. Melanom, pigment üreten hücreler olan melanositlerde genetik mutasyonların ortaya çıkmasından kaynaklanır. Ziziphus Jujube Mill., Asya'nın tropik/subtropikal iklime sahip bölgelerinde yetişir ve binlerce yıldır temel geleneksel halk ilacı olarak kullanılmaktadır. Bu çalışma, Ziziphus Jujube’nin melanom üzerindeki potansiyel antioksidan etkilerini belirlemek amacıyla yapılmıştır. Hücrelerin proliferasyonu, MTT analizi (kolorimetrik) ile ölçüldü. Melanom hücreleri, %100 konsantrasyonda Ziziphus Jujube esansiyel yağı ile üç saat muamele edildi. Total antioksidan ve oksidan kapasite, glutatyon ve nitrik oksit seviyeleri spektrofotometrik olarak belirlendi. Ziziphus Jujube, hücre çoğalmasını önlemiş ve toplam antioksidan kapasitesini artırmıştır. Sonuçlarımız, Ziziphus jujube'nin melanom hücrelerinde antioksidan seviyelerindeki düşüşü ve nitrik oksit seviyelerinin yükselmesini engellediğini göstermiştir. Ayrıca, Ziziphus Jujube uygulaması GSH düzeylerini iyileştirmiştir. Özetle, Ziziphus Jujube’nin melanom hücrelerinde potansiyel antioksidatif ve antiproliferatif etkiler gösterdiği sonucuna varılabilir. Son araştırmalar, Ziziphus Jujube meyvesinin tıbbi kullanımı için ayrıntılı bir etnofarmakolojik yaklaşım sunmaktadır.

Anahtar Kelimeler

• melanoma
• ziziphus jujube
• Antioksidanlar
• Oksidatif stres

The Potential Antioxidant Effects Of Ziziphus Jujube On Melanoma Cells

Öz

Cellular redox homeostasis is compromised when exposed to internal or external stress factors, resulting in the superiority of the oxidant. It is known that these reactive oxygen species play a critical role in the etiology of many serious diseases like diabetes and cancer. Melanoma is the deadliest form of skin cancer and its rates are currently increasing faster than other preventable cancers. Melanoma is caused by the appearance of genetic mutations in pigment-producing cells, melanocytes. Ziziphus jujube Mill. spreads in regions of Asia with tropical/subtropical climates and has been used for thnds of years as basic traditional folk medicine. This study was carried out to determine the potential antioxidant effects of Ziziphus jujube on melanoma. Survival of cells was measured by MTT assay (colorimetric). Melanoma cells were treated three hours with 100% concentration Ziziphus jujube essential oil. Total antioxidant and oxidant capacity, glutathione, and nitric oxide levels were determined by spectrophotometrically. Ziziphus jujube prevented cell proliferation and increased total antioxidant capacity. Our results indicated that Ziziphus jujube inhibited the decrease in antioxidant levels and elevation of nitric oxide levels in melanoma cells. Also, the Ziziphus jujube administration improved GSH levels. Briefly, it can be concluded that Ziziphus jujube showed potential antioxidative and antiproliferative effects in melanoma cells. Recent research provides a detailed ethnopharmacological approach for the medicinal use of Ziziphus jujube fruit.

Anahtar Kelimeler

• Antioxidants
• Melanoma
• Oxidative stress
• Ziziphus jujube

Teşekkür

ACKNOWLEDGEMENTS Cell culture studies of this research were performed at the Mustafa Kemal University Faculty of Veterinary.

Kaynakça

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