Determination of Antifungal Activity of Leaf Extracts from Hypericum ssp. Against Plant Pathogenic Fungi Fusarium oxysporum and Alternaria Alternata

Öz

In this study, the antifungal activity of ethanol extracts of three plant species of Hypericum perforatum, Hypericum scabrum and Hypericum origanifolium were evaluated for against two plant pathogenic fungal species of Fusarium oxysporum and Alternaria alternata.The antifungal activities of Hypericum ssp. extracts (3, 15, 45, 80, 120, 200, 240, 300 ppm) were tested against fungi. Ethanol 30% as control; Commercial fungicide (1 μg/mL) was used as a positive control. All Hypericum ssp. plant extracts were effective in different rates against the fungi depending on the doses. In addition, IC50 values of the three selected plant Hypericum ssp. leaf extract against pathogenic fungus F.oxysporum and A. alternata were determined with of probit analysis. In terms of the IC50 values, the antifungal activity of H. perforatum plant exract against F.oxysporum (122.525 μg/mL) > A.alternata (108.484 μg/mL). On the other hand, IC50 values of H. scabrum against A.alternata (126.390 μg/mL) > F.oxysporum (113.714 μg/mL). Moreover, the IC50 value of H. origanifolium against two pathogenic fungi was calculated as F.oxysporum (159.931 μg/mL) > A.alternata (55.759 μg/mL). Results showed that H.origanifolium has the best fungicidal activity, with IC50 value of 55.759 μg/mL against A.alternata and H.scabrum 113.717 μg/mL against F.oxysporum. However, the highest concentrations (300 ppm) caused completely inhibition in the both fungi mycelial growth followed by lower concentrations of plant extracts. A. alternata were the sensitive fungal species, while the F. oxysporum were a more resistant to the Hypericum ssp. extracts. Based on the antifungal activity tests, these plants extracts this may have effective as the new natural fungicide protecting crops against fungal diseases.

Anahtar Kelimeler

• • Hypericum ssp.
• • Fusarium oxysporum
• • Antifungal activity
• • Alternaria alternata

Kaynakça

1. Aljubiri, S. M., Mahgoub, S. A., Almansour, A. I., Shaaban, M., & Shaker, K. H. (2021). Isolation of diverse bioactive compounds from Euphorbia balsamifera: Cytotoxicity and antibacterial activity studies. Saudi journal of biological sciences, 28(1), 417 - 426.
2. Amin, A., Akbar, M., Khalil, T., Akram, W., & Ahmad, A. (2022). Antıfungal actıvıty of Alternanthera philoxeroides organıc solvent extracts against plant pathogenic fungı. Pak. J. Bot, 54 (1), 337-344.
3. Arlene, A. A., Prima, K. A., Utama, L., & Anggraini, S. A. (2015). The preliminary study of the dye extraction from the avocado seed using ultrasonic assisted extraction. Procedia Chemistry, 16, 334 - 340.
4. Bertoli, A., Çirak, C., & Seyis, F. (2015). Hypericum origanifolium Willd.: The essential oil composition of a new valuable species. Industrial Crops and Products, 77, 676 - 679.
5. Bilici S, Demir S, Boyno G, 2021. The Effects of Essential Oils and Arbuscular Mycorrhizal Fungi on Decay Disease (Fusarium oxysporum f. sp. radicis lycopersici Jarvis & Shoemaker) of Root and Root Collar of Tomato. Journal of the Institute of Science and Technology, 11(2): 857 - 868.
6. Carvalho, D. D. C., Alves, E., Camargos, R. B., Oliveira, D. F., Scolforo, J. R. S., de Carvalho, D. A., & Batista, T. R. S. (2011). Plant extracts to control Alternaria alternata in Murcott tangor fruits. Revista Iberoamericana de Micología, 28 (4), 173 - 178.
7. Castillo, F., Aguilar, C., Hernández, D., Gallegos, G., & Rodríguez, R. (2012). Antifungal properties of bioactive compounds from plants: INTECH Open Access Publisher.
8. Chahal, R., Nanda, A., Akkol, E. K., Sobarzo-Sánchez, E., Arya, A., Kaushik, D., . . . Mittal, V. (2021). Ageratum conyzoides L. and its secondary metabolites in the management of different fungal pathogens. Molecules, 26 (10), 2933.

9. Dadkhah, A., Fatemi, F., Alipour, M., Fatourehchi, S., & Parchini, F. S. (2015). Regulatory effect of Iranian Hypericum scabrum essential oils on hepatic metabolizing enzymes in rats treated by acetaminophen. Journal of Essential Oil Bearing Plants, 18 (2), 335 - 348.
10. Denaro, M., Smeriglio, A., Barreca, D., De Francesco, C., Occhiuto, C., Milano, G., & Trombetta, D. (2020). Antiviral activity of plants and their isolated bioactive compounds: An update. Phytotherapy Research, 34 (4), 742 - 768.
11. El-Shahir, A. A., El-Wakil, D. A., Abdel Latef, A. A. H., & Youssef, N. H. (2022). Bioactive Compounds and Antifungal Activity of Leaves and Fruits Methanolic Extracts of Ziziphus spina-christi L. Plants, 11 (6), 746.
12. Elshafie, H. S., Sakr, S., Bufo, S. A., & Camele, I. (2017). An attempt of biocontrol the tomato-wilt disease caused by Verticillium dahliae using Burkholderia gladioli pv. agaricicola and its bioactive secondary metabolites. International Journal of Plant Biology, 8 (1), 7263. Ergin, K. N., Karakaya, S., Göger, G., Sytar, O., Demirci, B., & Duman, H. (2022). Anatomical and Phytochemical Characteristics of Different Parts of Hypericum scabrum L. Extracts, Essential Oils, and Their Antimicrobial Potential. Molecules, 27 (4), 1228.
13. Erken, S., Malyer, H., Demirci, F., Demirci, B., & Baser, K. (2001). Chemical investigations on some Hypericum species growing in Turkey-I. Chemistry of Natural Compounds, 37 (5), 434-438.
14. Estevinho, B. N., Horciu, I.-L., Blaga, A.-C., & Rocha, F. (2021). Development of controlled delivery functional systems by microencapsulation of different extracts of plants: Hypericum perforatum L., Salvia officinalis L. and Syzygium aromaticum. Food and Bioprocess Technology, 14 (8), 1503 - 1517.
15. Gazeran, S., Tajalli, F., & Mohamadi Sani, A. (2016). Effect of ultrasonic extraction on qualitative parameters of saffron edible extract. Journal of Essential Oil Bearing Plants, 19 (5), 1286 - 1291.
16. Gibert, S., Edel-Hermann, V., Gautheron, E., Gautheron, N., Sol, J.-M., Capelle, G., . . . Steinberg, C. (2022). First Report of Fusarium avenaceum, Fusarium oxysporum, Fusarium redolens, and Fusarium solani causing root rot in pea in France. Plant Disease, 106 (4), 1297.
17. Heshmati, A., Alikhani, M., Godarzi, M., & Sadeghimanesh, M. (2018). The Evaluation of Antioxidant and Antimicrobial Activities of Essential Oil and Aqueous, Methanol, Ethanol, Ethyl Acetate and Acetone Extract of Hypericum scabrum. International Journal of Nutrition and Food Engineering, 12 (2), 47 - 51.
18. Jobling, J. (2000). Essential oils: A new idea for postharvest disease control. Good fruit and vegetables magazine, 11 (3), 50 - 54.
19. Khorshidi, J., Morshedloo, M. R., & Moradi, S. (2020). Essential oil composition of three Iranian Hypericum species collected from different habitat conditions. Biocatalysis and Agricultural Biotechnology, 28, 101755.
20. Lowe, H., Steele, B., Bryant, J., Fouad, E., Toyang, N., & Ngwa, W. (2021). Antiviral activity of Jamaican medicinal plants and isolated bioactive compounds. Molecules, 26 (3), 607.
21. Morshedloo, M. R., Ebadi, A., Maggi, F., Fattahi, R., Yazdani, D., & Jafari, M. (2015). Chemical characterization of the essential oil compositions from Iranian populations of Hypericum perforatum L. Industrial Crops and Products, 76, 565 - 573.
22. Nieder, R., Benbi, D. K., & Reichl, F. X. (2018). Health risks associated with pesticides in soils. In Soil components and human health (pp. 503-573): Springer.
23. Nwosu, M. O., & Okafor, J. I. (1995). Preliminary studies of the antifungal activities of some medicinal plants against Basidiobolus and some other pathogenic fungi: Vorläufige Studien zur antimyzetischen Aktivität einiger offizineller Pflanzen auf Basidiobolus und andere pathogene Pilze. Mycoses, 38 (5‐6), 191 - 195.
24. Onaran, A., & Yılar, M. (2012). Antifungal activity of Trachystemon orientalis L. aqueous extracts against plant pathogens. Journal of Food, Agriculture & Environment, 10 (3 & 4), 287 - 291.
25. Parry, D., Jenkinson, P., & McLeod, L. (1995). Fusarium ear blight (scab) in small grain cereals—a review. Plant pathology, 44 (2), 207 - 238.
26. Riaz T, Nawaz Khan S, Javaid A. (2010). Management of corm-rot disease of gladiolus by plant extracts. Natural Product Research, 24 (12), 1131 - 1138.
27. Roy, C. K., Akter, N., Sarkar, M. K., Pk, M. U., Begum, N., Zenat, E. A., & Jahan, M. A. (2019). Control of early blight of tomato caused by and screening of tomato varieties against the pathogen. The Open Microbiology Journal, 13 (1).
28. Seyrekoğlu, F., & Temiz, H. (2020). Effect of Extraction Conditions on the Phenolic Content and DPPH Radical Scavenging Activity of Hypericum perforatum L. Turkish Journal of Agriculture-Food Science and Technology, 8 (1), 226 - 229.
29. Syahir, A., Sulaiman, S., Mel, M., Othman, M., & Sulaiman, S. Z. (2020). An Overview: Analysis of ultrasonic-assisted extraction’s parameters and its process. Paper presented at the IOP Conference Series: Materials Science and Engineering.
30. Şesan, T. E., Enache, E., Iacomi, B. M., Oprea, M., Oancea, F., & Iacomi, C. (2017). In vitro antifungal activity of some plant extracts against Fusarium oxysporum in blackcurrant (Ribes nigrum L.). Acta Scientiarum Polonorum Hortorum Cultus, 16 (6), 167 - 176.
31. Wang, F., Saito, S., Michailides, T. J., & Xiao, C.-L. (2021). Postharvest use of natamycin to control Alternaria rot on blueberry fruit caused by Alternaria alternata and A. arborescens. Postharvest Biology and Technology, 172, 111383.