Eber Gölü Prokaryotik Çeşitliliğinin Metagenomik Çalışmasıyla Karakterizasyonu

Öz

Eber Gölü, Türkiye'de göçmen kuşların geçiş rotası üzerinde yer almakta ve sazlık alanları sayesinde birçok kuş türüne doğal üreme ve yaşam alanı sağlamaktadır. Ülkemizde bu kadar önemli bir göl olmasına rağmen prokaryotik çeşitlilik konusunda yeterli çalışma olmadığı görülmüştür. Metagenomik analiz DNA izolasyonu ile elde edilen mikroorganizmaların türlerini, genetik yapılarını, fonksiyonel rollerini ve insan ile ekolojik sağlık üzerindeki etkilerini belirlemeyi mümkün kılmaktadır. Yeni nesil dizileme (YND), birçok DNA veya RNA örneğinin eşzamanlı olarak yüksek hızda ve yüksek hassasiyetle dizilenmesini mümkün kılan, modern bir DNA/RNA dizileme teknolojisidir. Bu çalışmamızda Eber Gölü'ne ait 16S ribozomal DNA (rDNA) (V3-V4 bölgeleri) dizilerinin metagenomik analizi için yüksek verimli YND yöntemleri kullanılmıştır. Prokaryotik çeşitliliği incelemek amacıyla MOTHUR yazılımı kullanılarak Illumina NovaSeq teknolojisi ile elde edilen dizi verileri analiz edilmiştir. Analizler sonucu Proteobacteria, Verrucomicrobia, Bacteroidetes ve Actinobacteria Şubelerinin baskın olduğu ortaya çıkarılarak Eber Gölü'ndeki prokaryotik çeşitlilik detaylı bir şekilde karakterize edilmiştir.

Anahtar Kelimeler

• Prokaryotik çeşitlilik
• metagenomik
• ekoloji
• 16s rRNA
• İllumina dizileme

Characterization of Prokaryotic Diversity in Eber Lake through Metagenomic Analysis

Öz

Eber Lake is located on the migration route of birds in Turkey and provides natural breeding and habitat for many bird species thanks to its reed areas. Despite being such an important lake in our country, there has been insufficient research on prokaryotic diversity. Metagenomic analysis enables the determination of the species, genetic structures, functional roles, and their effects on human and ecological health of microorganisms obtained through DNA isolation. Next-generation sequencing (NGS) is a modern DNA/RNA sequencing technology that allows for the simultaneous high-speed and high-precision sequencing of many DNA or RNA samples. In this study, we used high-throughput NGS methods for metagenomic analysis of 16S ribosomal DNA (rDNA) (V3-V4 regions) sequences from Eber Lake. The obtained sequence data using Illumina NovaSeq technology was analyzed with MOTHUR software to investigate prokaryotic diversity. The analysis revealed that the Proteobacteria, Verrucomicrobia, Bacteroidetes, and Actinobacteria phyla were dominant, and the prokaryotic diversity in Eber Lake was characterized in detail.

Anahtar Kelimeler

• metagenomics
• ecology
• prokaryotic diversity
• illimina Sequencing
• 16s rRNA

Kaynakça

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