Tüylü Çayın (Stachys lavandulifolia) Fitokimyasal Analizi ve Antioksidan, Antikolinesteraz ve Antiaterojenik Aktivitesi

Öz

Stachys lavandulifolia Vahl. etnobotanikte önemli yeri olan Lamiaceae familyasına ait çiçekli bitki ailesindendir. Bu çalışmada bitkinin fitokimyasal kompozisyonu, asetilkolinesteraz (AChE) inhibisyonu, antiaterosklerotik aktivite için paraoksonaz (hPON 1) inhibisyonu ve antioksidan kapasitesi araştırıldı. Fitokimyasal içerik LC-MS/MS sistemi ile enzim inhibisyonu ve antioksidan kapasite çalışmaları ise spektrofotometre ile belirlendi. S. lavandulifolia ekstraktlarının (metanol, hekzan ve su) antioksidan kapasitesi ABTS, DPPH, FRAP ve CUPRAC yöntemleri uygulanarak belirlendi. S. lavandulifolia 'nın metanol ekstraktı AChE üzerinde önemli inhibisyon sergiledi (metanol ekstraktı için IC50 değeri 0.105± 0.17 mg/mL (R2:0.978)). Buna karşılık, S. lavandulifolia'nın metanol ve su ekstreleri hPON 1 üzerinde inhibisyon etkisi göstermedi. ABTS için en yüksek aktivite metanol ekstresinde %23.42 ve DPPH aktivitesi için metanol ekstresinde %50.07 olarak belirlendi. Metal indirgeme gücü deneyinde, FRAP su ekstraktı için 0.233± 0.47 ve CUPRAC metanol ekstraktı için 0.587±1.52 absorbans olarak tespit edildi. Bitkinin metanol ekstraktında LC-MS/MS analizlerine göre luteolin, fumarik asit, kafeik asit, siringik asit, hidroksibenzoik asit, kuersetin, salisilik asit, gallik asit, kateşin hidrat ve asetohidroksamik asit saptanmıştır. Sonuç olarak, antioksidan, anti-aterojenik ve anti-nörodejeneratif özelliklere sahip olan S. lavandulifolia, Alzheimer hastalarında kullanılan sentetik ilaçlar yerine doğal bir ilaç olarak kullanılma potansiyeline sahiptir.

Anahtar Kelimeler

• Antioksidan
• Asetilkolinesteraz
• Paraoksonaz
• LC-MS/MS
• Stachys lavandulifolia

Phytochemical Analysis and Antioxidant, Anticholinesterase and Antiatherogenic Activity of Hairy Tea (Stachys lavandulifolia)

Öz

Stachys lavandulifolia Vahl. is a flowering plant family belonging to the Lamiaceae family, which has a great place in ethnobotany. In this study, the phytochemical composition, inhibition of acetylcholinesterase (AChE) and paraoxonase (hPON 1) for antiatherosclerotic activity, and antioxidant capacity of the plant were investigated. Phytochemical content was determined by LC-MS/MS system, enzyme inhibition, and antioxidant capacity studies were determined by spectrophotometer. The antioxidant capacity of S. lavandulifolia extracts (methanol, hexane, and water) was determined by applying ABTS, DPPH, FRAP, and CUPRAC methods. The methanol extract of S. lavandulifolia exhibited significant inhibition of AChE (IC50 value for methanol extract 0.105±0.17 mg/mL (R2:0.978)). In contrast, methanol and water extracts of S. lavandulifolia showed no inhibitory effect on hPON 1. The highest activity for ABTS was 23.42% in methanol extract and 50.07% for DPPH activity in methanol extract. In the metal-reducing power test, the absorbance was 0.233±0.47 for FRAP water extract and 0.587±1.52 for CUPRAC methanol extract. According to LC-MS/MS analysis, luteolin, fumaric acid, caffeic acid, syringic acid, hydroxybenzoic acid, quercetin, salicylic acid, gallic acid, catechin hydrate, and acetohydroxamic acid were determined in the methanol extract of the plant. In conclusion, S. lavandulifolia, which has antioxidant, anti-atherogenic, and anti-neurodegenerative properties, has the potential to be used as a natural medicine instead of synthetic drugs used in Alzheimer's patients.

Anahtar Kelimeler

• Antioxidant
• Acetylcholinesterase
• Paraoxonase
• Stachys lavandulifolia
• LC-MS/MS

Kaynakça

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